Nau'ikan Kwayar cutar Papilloma ta ɗan adam guda 15 masu haɗarin gaske E6/E7 Gene mRNA
Sunan samfurin
Nau'ikan Kayan Gano MRNA na Halittar HWTS-CC005A-15 Masu Haɗarin Cutar Papilloma ta Ɗan Adam E6/E7 (Fluorescence PCR)
Ilimin Cututtuka
Ciwon daji na mahaifa yana ɗaya daga cikin nau'ikan ciwon daji na mata da aka fi sani a duniya, kuma faruwarsa tana da alaƙa da ƙwayoyin cuta na papilloma na ɗan adam (HPV), amma ƙaramin kaso ne kawai na kamuwa da cutar HPV zai iya zama ciwon daji. HPV mai haɗari yana shafar ƙwayoyin epithelial na mahaifa kuma yana samar da oncoproteins guda biyu, E6 da E7. Wannan furotin na iya shafar nau'ikan furotin na ƙwayoyin halitta (kamar furotin masu rage ciwace-ciwacen pRB da p53), tsawaita zagayowar ƙwayoyin halitta, yana shafar haɗakar DNA da kwanciyar hankali na kwayoyin halitta, da kuma tsoma baki ga martanin rigakafi na rigakafi da na rigakafi.
Tashar
| Tashar | Bangaren | An gwada nau'in halittar jini |
| FAM | Mai Rage Radadin HPV 1 | HPV16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Kwayar halittar β-actin ta ɗan adam | |
| FAM | Mai Rage Radadin HPV 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Kwayar halittar ɗan adam ta INS |
Sigogi na Fasaha
| Ajiya | Ruwa: ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 9 |
| Nau'in Samfuri | Swab na mahaifa |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Kwafi 500/mL |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 Tsarin PCR na Ainihin Lokaci na QuantStudio®5 Tsarin PCR na SLAN-96P na Gaskiya Tsarin PCR na LightCycler®480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokaci Mai Keke Mai Zafi na MA-6000 na Ainihin Lokaci Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Shawarar sinadarin cirewa: Kit ɗin DNA/RNA na Macro & Micro-Test Viral (HWTS-3020-50-HPV15) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ya kamata a gudanar da cirewar bisa ga umarnin amfani. Ƙarar fitar da ruwa da aka ba da shawarar ita ce 50μL. Idan samfurin bai narke gaba ɗaya ba, mayar da shi zuwa mataki na 4 don sake narkewa. Sannan a gwada bisa ga umarnin amfani.
Maganin cirewa da aka ba da shawarar: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431). Ya kamata a gudanar da cirewar bisa ga umarnin amfani da shi sosai (A mataki na 5, ninka yawan maganin aiki na DNaseI, wato, a ɗauki 20μL na maganin DNase-Free DNaseI (1500U) a cikin sabon bututun centrifuge na RNase-Free, a ƙara 60μL na RDD buffer, sannan a gauraya a hankali). Ƙarar fitar da samfurin da aka ba da shawarar shine 60μL. Idan samfurin bai narke gaba ɗaya ba, a mayar da shi zuwa mataki na 5 don sake narkewa. Sannan a gwada bisa ga umarnin amfani.
