Nau'in Haɗin mutum 15 masu haɗarin Papillomus E6 / E7 Gene Gene Gene Gene
Sunan Samfuta
HWTS-CC005A-15 nau'ikan hadarin papillomous E6 / e7 Gene Gene Mrna Gano Kit (mai kyalli PCR)
TopideMology
Cutar Cervical tana daya daga cikin nau'ikan nau'ikan mata na mata, da kuma abin da ya faru na da alaƙa da papillomiviruse na mutane (hpv), amma karamin rabo na HPV kamuwa da cutar kansa. High-hadarin HPV yana cutar da ƙwayoyin sel na mahaifa da kuma samar da upcoproteins guda biyu, E6 da E7. Wannan furotin na iya shafar sunadarai iri-iri (kamar suzan kariyar kayan aikin prb da kuma p53), tsawaita sake zagayowar kwayar halitta, kuma tsangwama na DNOVIher da amsar rigakafi.
Hanya
| Hanya | Kayan wucin gadi | Genotype Genotype |
| Famfo | HPV reffer 1 | HPV16,31,33,35,51,51,58 |
| Vic / Hex | Gene β-Actin Gene | |
| Famfo | HPV dauki Buffer 2 | HPV 18,39,45,53,53,59,66,68 |
| Vic / Hex | Dan kasar IS |
Sigogi na fasaha
| Ajiya | Liquid: ≤-18 ℃ |
| Shelf-rayuwa | Watanni 9 |
| Nau'in samfur | Swab swab |
| Ct | ≤38 |
| CV | <5.0% |
| Lod | 500 kwafin / ml |
| Kayan aiki | Amfani da biosystems 7500 na lokaci-lokaci tsarin tsariAmfani da biosystems 7500 mai sauri na lokaci-lokaci tsarin tsari QUMSTUDIO®5 Tsarin aiki na lokaci-lokaci Slan-96p Real-Lokaci na Real-Lokaci Tsarin PCR na Haske Linegene 9600 da tsarin ganowa na yau da kullun PCR Ma-6000 na lokaci-lokaci na zamani Tsarin cfx96 na lokaci-lokaci na lokaci-lokaci Biorad cfx opus 96 real-lokaci-lokaci tsarin PCR |
Aiki kwarara
Gwajin hakar da aka kawo: Macro & Micro-Gwajin hoto ko bidiyo . Da shawarar ƙa'idar ƙira shine 50μl. Idan samfurin ba a narkewa ba gaba daya, mayar da shi zuwa mataki na 4 don juyawa. Sannan gwadawa bisa umarnin amfani.
Farawar hakar da aka yada: Rapperep tsarkakakken ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar ƙasa (DP431). Ya kamata a gudanar da hakar bisa ga umarnin don amfani sosai (a mataki na 5, ninka yawan maganin aikin Dnasei (1500u) a cikin sabon tube na rnase-free. Addara 60μl na RDD Buffer, kuma Mix a hankali). Shawarwarin ƙa'idar ƙira shine 60μL. Idan samfurin ba a narkewa ba gaba daya, mayar da shi zuwa mataki 5 don juyawa. Sannan gwadawa bisa umarnin amfani.
