Nau'o'i 28 na Cutar Cutar Papilloma na Dan Adam (16/18 Bugawa) Nucleic Acid
Sunan samfur
Nau'ikan HWTS-CC006A-28 Nau'in Cutar Papilloma na ɗan adam mai haɗari (16/18 Bugawa) Kayan Gane Acid Nucleic (Fluorescence PCR)
Epidemiology
Ciwon daji na mahaifa yana daya daga cikin muggan ciwace-ciwacen da ake samu a cikin mahaifar mace.Nazarin ya nuna cewa ciwon daji na HPV da cututtuka masu yawa suna daya daga cikin manyan abubuwan da ke haifar da ciwon daji na mahaifa.A halin yanzu, ana samun ingantattun magunguna waɗanda har yanzu ba su da ciwon sankarar mahaifa da HPV ke haifarwa, don haka gano wuri da rigakafin kamuwa da cutar sankarar mahaifa ta HPV shine mabuɗin hana kansar mahaifa.Yana da matukar mahimmanci don kafa gwaji mai sauƙi, ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ilimin etiology don ganowar asibiti da kuma kula da kansar mahaifa.
Tashoshi
| Haɗin kai | Tashoshi | Nau'in |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Ikon Cikin Gida | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | Ruwa: ≤-18℃ |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | Swab Cervical, Farji Swab, Fitsari |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300 Kwafi/ml |
| Abubuwan da ake Aiwatar da su | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Real-Time PCR tsarin LineGene 9600 Plus Tsarin Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Shawarar hakar reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za a iya amfani da tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ƙara 200μL na al'ada saline don sake dakatar da pellet a mataki na 2.1, sannan kuma za a gudanar da hakar bisa ga umarnin. zuwa umarnin don amfani da wannan reagent hakar.Adadin da aka ba da shawarar shine 80μL.
Nasihar hakar reagent: QIAamp DNA Mini Kit (51304) ko Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Ƙara 200μL na saline na al'ada don sake dakatar da pellet a mataki na 2.1, sannan ya kamata a gudanar da hakar bisa ga umarnin don amfani da wannan reagent na hakar.Girman samfurin da aka fitar na samfurori duk 200μL ne, kuma ƙimar da aka ba da shawarar shine 100μL.
