Tsarin Halittar ALDH
Sunan samfurin
HWTS-GE015Kayan Gano Halittar Halittar ALDH (ARMS -PCR)
Ilimin Cututtuka
Kwayar halittar ALDH2 (acetaldehyde dehydrogenase 2), tana nan a kan kwayar halittar ɗan adam ta 12. ALDH2 yana da aikin esterase, dehydrogenase da reductase a lokaci guda. Bincike ya nuna cewa ALDH2 wani enzyme ne na metabolism na nitroglycerin, wanda ke canza nitroglycerin zuwa nitric oxide, ta haka yana kwantar da jijiyoyin jini da inganta matsalolin kwararar jini. Duk da haka, akwai polymorphisms a cikin kwayar halittar ALDH2, waɗanda galibi suka fi yawa a Gabashin Asiya. Kwayar halittar ALDH2*1/*1 GG mai nau'in daji tana da ƙarfin metabolism mai ƙarfi, yayin da nau'in heterozygous yana da kashi 6% kawai na aikin enzyme na nau'in daji, kuma nau'in homozygous mai canza suna da kusan babu aikin enzyme, tare da metabolism mai rauni sosai kuma ba zai iya cimma tasirin da ake so ba, don haka yana haifar da lahani ga jikin ɗan adam.
Tashar
| FAM | ALDH2 |
| ROX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | Jini mai hana yin tari da EDTA |
| CV | <5.0% |
| LoD | 103Kwafi/mL |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 QuantStudio®Tsarin PCR guda 5 na Ainihin Lokaci Tsarin PCR na SLAN-96P na Gaskiya LightCycler®Tsarin PCR na 480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokaci Mai Keke Mai Zafi na MA-6000 na Ainihin Lokaci Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Shawarar sinadaran cirewa: yi amfani da Kayan Hana Jini na Jini (DP318) na Tiangen Biotech (Beijing) Co., Ltd. ko Kayan Hana Jini na Jini (A1120) na Promega don cire jinin da aka hana yin coagulation na EDTA.
Shawarar sinadaran cirewa: Kit ɗin DNA/RNA na Macro & Micro-Test General (HWTS-3019) (wanda za a iya amfani da shi tare da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ya kamata a gudanar da cirewar bisa ga umarnin da aka bayar. Girman fitar da ruwa da aka ba da shawarar shine100μL.
