ALDH Genetic Polymorphism
Sunan samfur
HWTS-GE015ALDH Genetic Polymorphism Kit ɗin Ganewa (ARMS -PCR)
Epidemiology
ALDH2 gene (acetaldehyde dehydrogenase 2), yana samuwa akan chromosome ɗan adam 12. ALDH2 yana da esterase, dehydrogenase da reductase aiki a lokaci guda. Nazarin ya nuna cewa ALDH2 shine enzyme na rayuwa na nitroglycerin, wanda ke canza nitroglycerin zuwa nitric oxide, ta haka ne ya kwantar da jini da kuma inganta matsalolin jini. Duk da haka, akwai polymorphisms a cikin al'adar ALDH2, waɗanda aka fi mayar da hankali a Gabashin Asiya. Nau'in daji ALDH2 * 1/* 1 GG yana da ƙarfi mai ƙarfi na rayuwa, yayin da nau'in heterozygous yana da 6% kawai na aikin enzyme na daji, kuma nau'in mutant na homozygous yana da kusan aikin enzyme na sifili, tare da metabolism mai rauni sosai kuma ba zai iya cimma tasirin da ake so ba, don haka yana haifar da lahani ga jikin ɗan adam.
Tashoshi
| FAM | ALDH2 |
| ROX | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | EDTA anticoagulated jini |
| CV | <5.0 da |
| LoD | 103Kwafi/ml |
| Abubuwan da ake Aiwatar da su | Aiwatar da Tsarin Halitta 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Real-Time PCR tsarin LineGene 9600 Plus Tsarin Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Abubuwan da aka ba da shawarar hakar reagents: yi amfani da Kit ɗin Haɗin Jini na DNA (DP318) na Tiangen Biotech (Beijing) Co., Ltd. ko Kit ɗin Haɗin Jini (A1120) ta Promega don cire EDTA anticoagulated jini Genomic DNA.
Shawarar hakar reagents: Macro & Micro-Test Janar DNA / RNA Kit (HWTS-3019) (wanda za a iya amfani da tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The hakar ya kamata a gudanar bisa ga umarnin. Adadin da aka ba da shawarar shine100 μl.
