Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Sunan samfur
HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Kit ɗin Ganewa (Fluorescence PCR)
Epidemiology
Kwayoyin rigakafin Carbapenem sune maganin rigakafi na β-lactam na al'ada tare da mafi girman nau'in ƙwayoyin cuta da kuma aikin kashe kwayoyin cuta.Saboda kwanciyar hankali ga β-lactamase da ƙananan ƙwayar cuta, ya zama ɗaya daga cikin mahimman magungunan ƙwayoyin cuta don maganin cututtuka masu tsanani.Carbapenems suna da ƙarfi sosai ga plasmid-mediated extended-spectrum β-lactamases (ESBLs), chromosomes, da plasmid-mediated cephalosporinases (AmpC enzymes).
Tashoshi
| PCR-Mix 1 | PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Ikon Cikin Gida | Ikon Cikin Gida |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | Sputum, tsaftataccen mazauna, swab na dubura |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/ml |
| Musamman | a) Kit ɗin yana gano ƙayyadaddun nassoshi mara kyau na kamfani, kuma sakamakon ya dace da buƙatun nassoshi masu dacewa. b) Sakamakon gwajin gwagwarmayar giciye ya nuna cewa wannan kit ɗin ba shi da amsawa tare da sauran cututtuka na numfashi, irin su Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Kleb junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, na numfashi adenovirus, Enterococcus, ko samfurori dauke da sauran kwayoyi-resistant kwayoyin CTX, mecA, TEM, SHV. c) Anti-tsangwama: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin da aka zaba don tsangwama gwajin, da kuma sakamakon nuna cewa sama da aka ambata tsoma baki abubuwa ba su da wani tsoma baki abu. don gano ƙwayoyin juriya na carbapenem KPC, NDM, OXA48, OXA23, VIM, da IMP. |
| Abubuwan da ake Aiwatar da su | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Tsarin PCR na Gaskiya Tsarin Gano PCR na LineGene 9600 PlusFQD-96A,HangzhouFasahar Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Zabin 1.
Nasihar hakar reagent: Macro & Micro-Test Janar DNA/RNA Kit (HWTS-301)9-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (wanda za'a iya amfani dashi tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ƙara 200μL na al'ada Saline zuwa da thallus hazo.Matakan da suka biyo baya yakamata su bi umarnin cirewa, kuma ƙarar da aka ba da shawarar shine100 μl.
Zabin 2.
Shawarar hakar reagent: Nucleic Acid Extraction ko Purification Reagent (YDP302) ta Tiangen Biotech (Beijing) Co., Ltd. Ya kamata a fara hakar a tsantsa daidai da mataki na 2 na umarnin don amfani (ƙara 200μL na buffer GA ga hazo mai thallus. , da girgiza har sai an dakatar da thallus gaba daya).Yi amfani da ruwan kyauta na RNase/DNase don haɓakawa, kuma ƙarar haɓakar da aka yaba shine 100μL.
Zabin 3.
Nasihar hakar reagent: Macro & Micro-Test Samfurin Sakin Reagent.Ana buƙatar wanke samfurin sputum ta ƙara 1mL na saline na yau da kullum zuwa sama da aka ambata a sama na thallus precipitate, centrifuged a 13000r/min na minti 5, kuma an zubar da maɗaukaki (a kiyaye 10-20µL na supernatant).Don tsarkakakken mallaka da swab na dubura, ƙara 50μL na samfurin sakewa kai tsaye zuwa abin da aka ambata na thallus precipitate na sama, kuma matakan da suka biyo baya yakamata a fitar dasu bisa ga umarnin don amfani.
