Tsarin Juriyar Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Sunan samfurin
Kayan Ganowa na HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) (Fluorescence PCR)
Ilimin Cututtuka
Magungunan rigakafi na Carbapenem maganin rigakafi ne na β-lactam marasa tsari waɗanda ke da faɗaɗɗen bakan maganin kashe ƙwayoyin cuta da kuma ƙarfin aikin kashe ƙwayoyin cuta. Saboda daidaitonsa ga β-lactamase da ƙarancin guba, ya zama ɗaya daga cikin mahimman magungunan kashe ƙwayoyin cuta don magance cututtukan ƙwayoyin cuta masu tsanani. Carbapenems suna da ƙarfi sosai ga β-lactamases masu tsayin daka (ESBLs), chromosomes, da cephalosporinases masu tsayin daka (enzymes na AmpC).
Tashar
| PCR-Mix 1 | PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Sarrafa Cikin Gida | Sarrafa Cikin Gida |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | Maniyyi, tarin ƙwayoyin halitta, maɓalli na dubura |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Takamaiman Bayani | a) Kayan aikin yana gano ma'aunin ma'auni mara kyau na kamfanin, kuma sakamakon ya cika buƙatun ma'aunin da suka dace. b) Sakamakon gwajin amsawar ƙwayoyin cuta ya nuna cewa wannan kayan aikin ba shi da wani haɗin gwiwa da wasu ƙwayoyin cuta na numfashi, kamar su Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, ko samfuran da ke ɗauke da wasu kwayoyin halitta masu jure wa magani CTX, mecA, SME, SHV, TEM, da sauransu. c) Maganin tsangwama: An zaɓi Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin don gwajin tsangwama, kuma sakamakon ya nuna cewa abubuwan da aka ambata a sama ba su da wani martani ga gano kwayoyin halittar juriyar carbapenem KPC, NDM, OXA48, OXA23, VIM, da IMP. |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR na Ainihin Lokaci na QuantStudio®5 Tsarin PCR na SLAN-96P na Gaskiya (Hongshi Medical Technology Co., Ltd.) Tsarin PCR na LightCycler®480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus (FQD-96A，HangzhouFasahar Bioer) MA-6000 Mai Keke Mai Yawan Zafi na Ainihin Lokaci (Suzhou Molarray Co., Ltd.) Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Zaɓi na 1.
Shawarar da aka ba da shawarar cirewa: Kit ɗin DNA/RNA na Janar na Macro & Micro-Test (HWTS-301)9-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (wanda za a iya amfani da shi tare da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ƙara 200μL na gishirin yau da kullun a cikin thallus precipitate. Matakan da ke gaba ya kamata su bi umarnin don cirewa, kuma ƙarar fitar da ruwa da aka ba da shawarar shine100μL.
Zaɓi na 2.
Shawarar sinadarin cirewa: Cire sinadarin Nucleic Acid ko Tsarkakewa (YDP302) daga Tiangen Biotech (Beijing) Co., Ltd. Ya kamata a fara cirewar bisa ga mataki na 2 na umarnin amfani (ƙara 200μL na buffer GA zuwa ga thallus precipitate, sannan a girgiza har sai thallus ɗin ya tsaya gaba ɗaya). Yi amfani da ruwan da ba shi da RNase/DNase don cirewa, kuma girman fitarwar da aka ƙayyade shine 100μL.
Zaɓi na 3.
Maganin cirewa da aka ba da shawarar: Maganin sake fitar da samfurin Macro & Micro-Test. Ana buƙatar a wanke samfurin maniyyi ta hanyar ƙara 1mL na saline na yau da kullun zuwa ga sinadarin thallus da aka yi wa magani a sama, a saka shi a centrifuge a 13000r/min na tsawon mintuna 5, sannan a jefar da sinadarin (a ajiye 10-20µL na sinadarin cirewa). Don tsaftace colony da dubura, a ƙara 50μL na sinadarin cirewa kai tsaye zuwa ga sinadarin thallus da aka yi wa magani a sama, kuma a cire matakan da ke gaba bisa ga umarnin amfani.
