Gene Carbipenem

Carbipeneem resistance Gene (KPC / NDM / Oxa 48 / VIM / VIM / Shahararren) hoton da aka buga

A takaice bayanin:

Ana amfani da wannan kit don gano abubuwan da ke cikin Carbapenem na Carbupenem na Carbatum, wanda ya hada da samfuran da suka dace da shi, oxcu48 (oxa48 (oxc48 (oxa48), Oxa23 (oxacillinaste 23), vim (verona Ifipenemase), da kuma nuna (ifipenemase).

Aika imel zuwa gare mu

Cikakken Bayani

Tags samfurin

Sunan Samfuta

HWTS-ot045 CarbalibeM Jinare Gene (KPC / NDM / Oxa 48 / VIM / VIM / M)

TopideMology

Carbipeneem kwayoyin cuta na carbabbotics sune cututtukan ƙwayar cuta β-lactam tare da manyan ƙwayoyin cuta na ƙwayoyin cuta na ƙwayoyin cuta. Saboda kwanciyar hankali zuwa β-lacamase da ƙarancin guba, ya zama ɗayan mahimman magunguna don lura da cututtukan cututtukan cuta. Carbapeenems suna da tabbaci sosai ga plasmid-stectrum β-lactamases (Esbls), chroms), chromsomes, da plasmisoSimed cafalspormes (ampc enzymes).

Hanya

	PCR-Mix 1	PCR-Mix 2
Famfo	Ji	Ɓata
Vic / Hex	Kula da ciki	Kula da ciki
Cy5	Ndm	Kpc
Rox	Xafan xa48	Oxa23

Sigogi na fasaha

Ajiya	≤-18 ℃
Shelf-rayuwa	Watanni 12
Nau'in samfur	Sputum, mazauna mazauna, gefen swab
Ct	≤36
CV	≤5.0%
Lod	10³CFU / ML
BAYANIN	A) Kit ɗin yana gano ƙa'idar kamfanin mara kyau nassoshi, kuma sakamakon ya haɗu da buƙatun na nassoshi masu dacewa. b) Sakamakon gwajin bayan gida ya nuna cewa wannan kit ɗin bashi da giciye shi da sauran cututtukan ƙwayoyin cuta, kamar yadda Klobylococcus Aureus, Klibseliella Oxytoca, Hamiphilus Munii na yau da kullun, At Ainetobater Junii, At Atetobcer Jiicai, Adida Albicans, Cigida Albicans, Cindiga da sauransu c) tsangwama: mucin, minocycline, an zaɓi Clindamycin, EFVOFLOXAZIN, LEVOFLOXAZONE, abubuwa masu tsoma baki ba su da hakkin tsangwama da aka ambata a sama zuwa gano Carbipeneem Jarurruka na kwayoyin kpc, ndm, oxa48, oxa23, vim, da kuma rawar jiki.
Kayan aiki	Amfani da biosystems 7500 Tsarin aiki na lokaci-lokaci QUMSTUDIO®5 Tsarin aiki na lokaci-lokaci Slan-96p Real-Real-Lokaci na Real-Lokaci na Real-Lokaci (Kiwon Kuriyar Lantarki na Hongshi Co., Ltd.) Tsarin PCR na Haske Layin 9600 da wani tsarin ganowa na yau da kullun PCR (FQD-96A,HangzhouFasaha ta Bioer) Ma-6000 Real-Time Real-Time Streral Cycler (Suzhou Moharray Co., Ltd.) Tsarin cfx96 na lokaci-lokaci na lokaci-lokaci Biorad cfx opus 96 real-lokaci-lokaci tsarin PCR

Aiki kwarara

Zabi 1.

Mai ba da shawarar hakar mai kaya: Macro & Micro-Tuni Janar DNA / RNA9-50, hwts-3019-32, HWTS-3019-48, hwts-3019-96) (wanda za a iya amfani da shi tare da Macro & Micro-4 acid atomatik (hwts-3006b)) ta jiangen-jita-jita-jita-jijiyoyi da Thallus ya hazo. Matakai na biyu ya kamata ya bi umarnin don hakar, da kuma shawarar ƙura ƙira shine100μl.

Zabin 2.

Mai ba da shawarar hakar da aka ba da shawarar: hakar kayan aiki , kuma girgiza har sai an dakatar da Thallus gaba daya). Yi amfani da rnase / dnase kyauta na ƙa'idodi, da kuma ƙimar ƙa'idar ƙa'idar da ta gabata shine 100μl.

Zabin 3.

Saki na hakar mai amfani: Macro & micro-gwajin saki saki. Samfurin sputum yana buƙatar wanke 1ml na saline 1ml na al'ada da aka ambata a sama, wanda aka ambata a cikin mintuna 13, kuma mai sawainan ya watsar (ku kiyaye 10-20μl na Supernatant). Don tsarkakakkiyar mulkuma da gefen swab, ƙara 50μl na saki saki na samarwa kai tsaye ga Thala-da aka ambata ya kamata a fitar gwargwadon koyarwa don amfani.

A baya: Muryheza kwayar cuta ta gama kai / H1 / H3

Next: Carbapeneemase

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