Kwayar cutar Dengue, Kwayar cutar Zika da Kwayar cutar Chikungunya Multiplex
Sunan samfurin
Kwayar cutar Dengue ta HWTS-FE040, Kwayar cutar Zika da Kwayar cutar Chikungunya Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Ilimin Cututtuka
Zazzabin Dengue (DF), wanda kamuwar kwayar cutar dengue (DENV) ke haifarwa, yana ɗaya daga cikin cututtukan da suka fi yaɗuwa arbovirus. Maganin yaɗuwarsa ya haɗa da Aedes aegypti da Aedes albopictus. DF galibi ya fi yaɗuwa a yankunan zafi da kuma yankunan da ke ƙarƙashin zafi. DENV na cikin flavivirus a ƙarƙashin flaviviridae, kuma ana iya rarraba shi zuwa nau'ikan serotypes guda 4 bisa ga antigen ɗin saman jiki. Bayyanar cututtukan DENV galibi sun haɗa da ciwon kai, zazzabi, rauni, faɗaɗa lymph node, leukopenia da sauransu, da zubar jini, girgiza, raunin hanta ko ma mutuwa a cikin mawuyacin hali. A cikin 'yan shekarun nan, sauyin yanayi, ƙaura zuwa birane, saurin haɓaka yawon buɗe ido da sauran abubuwa sun samar da yanayi mai sauri da dacewa don yaɗuwa da yaɗuwar DF, wanda ke haifar da ci gaba da faɗaɗa yankin annoba na DF.
Tashar
| FAM | Asid ɗin nucleic na DENV |
| ROX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | -18℃ |
| Tsawon lokacin shiryawa | Watanni 9 |
| Nau'in Samfuri | Sabon magani |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | Kwafi 500/mL |
| Takamaiman Bayani | Sakamakon gwajin tsangwama ya nuna cewa idan yawan bilirubin a cikin jini bai wuce 168.2μmol/ml ba, yawan haemoglobin da hemolysis ke samarwa bai wuce 130g/L ba, yawan lipids a cikin jini bai wuce 65mmol/ml ba, jimlar yawan IgG a cikin jini bai wuce 5mg/mL ba, babu wani tasiri ga kwayar cutar dengue, kwayar cutar Zika ko gano kwayar cutar chikungunya. An zaɓi kwayar cutar Hepatitis A, kwayar cutar Hepatitis B, kwayar cutar Hepatitis C, kwayar cutar Herpes, kwayar cutar Eastern equine encephalitis, kwayar cutar Hantavirus, kwayar cutar Bunya, kwayar cutar West Nile da samfuran kwayoyin halittar dan adam don gwajin amsawar giciye, kuma sakamakon ya nuna cewa babu wani martani tsakanin wannan kayan aiki da kwayoyin cutar da aka ambata a sama. |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 QuantStudio®Tsarin PCR guda 5 na Ainihin Lokaci LightCycler®Tsarin PCR na 480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokaci Mai Keke Mai Zafi na MA-6000 na Ainihin Lokaci Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Zaɓi na 1.
Kit ɗin DNA/RNA na ƙwayar cuta ta TIAmp (YDP315-R), kuma ya kamata a gudanar da cirewar bisa ga umarnin amfani. Yawan samfurin da aka cire shine 140μL, kuma adadin fitar da ruwa da aka ba da shawarar shine 60μL.
Zaɓi na 2.
Kit ɗin DNA/RNA na Macro & Micro-Gwajin Gabaɗaya (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za a iya amfani da shi tare da Macro & Micro-Gwajin atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ta Jiangsu Macro & Micro-Gwajin Med-Tech Co., Ltd., kuma ya kamata a gudanar da cirewar bisa ga umarnin amfani. Girman samfurin da aka cire shine 200μL, kuma girman fitarwa da aka ba da shawarar shine 80μL.
