Kwayar cutar Zika, cutar Zika da Chikungunya Virus da yawa
Sunan Samfuta
Kwayar cutar HWTS-Feu040, cutar ta Zika da cutar Chikungunya Virtis na gano kayan aikin acid (mai kyalli PCR)
TopideMology
Dengue zazzabi (DF), wanda cutar kwayar cuta ta haifar (Denv), tana daya daga cikin cutar arbovirus corwates. Matsakaiciyar watsa watsa kai ta hada da AEDES AEDELSCIG da AEDES Alpopictus. DF ne yafi ƙaddara a wurare masu zafi da kuma ƙasashe marasa ƙarfi. Denv nasa ne a karkashin Flaviviridae, kuma ana iya rarrabe shi zuwa 4 herotypes bisa ga antigen surfing. Clinical bayyanar da denv kamuwa da ciwon kai, zazzabi, rauni, zubar da jini, rauni, rauni, raunin hatsari ko ma mutuwa a lokuta masu tsaurara. A cikin 'yan shekarun nan, canjin yanayi, birni, saurin yawon shakatawa da sauran dalilai don watsa da yaduwar yankin dF.
Hanya
Famfo | Denv nucleic acid |
Rox | Kula da ciki |
Sigogi na fasaha
Ajiya | -18 ℃ |
Shelf-rayuwa | Watanni 9 |
Nau'in samfur | Sabo ne serum |
Ct | ≤38 |
CV | <5% |
Lod | 500 kwafin / ml |
BAYANIN | Sakamakon gwajin gwaji ya nuna cewa lokacin da aka tattara Bilirub a cikin Serum bai wuce 130Gysis ba sama da 60g / ml, jimlar Igg Taro a cikin Serum ba fiye da 5MG / ML, babu wani tasiri a kan ƙwayar cuta mai doro jeri, cutar Zika ko Gano Chikungunda Virus. Kwayar cutar hepatitis ta kwayar cuta, hepatitis C kwayar cuta, cutar herpates, da Enteren Enly Enes, da ta Enam da ke tattare da cutar ta kasar Buti da ta nuna cewa babu wani Cross Haske tsakanin wannan kit da cututtukan da aka ambata a sama. |
Kayan aiki | Amfani da biosystems 7500 na lokaci-lokaci tsarin tsari Amfani da biosystems 7500 mai sauri na lokaci-lokaci tsarin tsari Quicstatawa®5 Tsarin PCR na ainihi Laima®Tsarin PCR na Real-Lokaci Linegene 9600 da tsarin ganowa na yau da kullun PCR Ma-6000 na lokaci-lokaci na zamani Tsarin cfx96 na lokaci-lokaci na lokaci-lokaci Biorad cfx opus 96 real-lokaci-lokaci tsarin PCR |
Aiki kwarara
Zabi 1.
Tanamas cutar DNA / RNA KIT (YDP315-R), yakamata a gudanar da hakar a cikin tsayayyen koyarwa don amfani. Volumeara samfurin samfurin shine 140μL, da shawarar ƙura ƙira shine 60μL.
Zabin 2.
Kit ɗin Janar Macro & RNA (HWTS-30066C, hwts-3006b)) na Jiangara Macro & Micro-gwajin Med-Tech Co., Ya kamata a gudanar da hakar bisa ga koyarwar don amfani. Yawan samfurin shine 200μl, da shawarar ƙura ƙira shine 80μL.