Cutar Dengue, Cutar Zika da Chikungunya Virus Multiplex
Sunan samfur
HWTS-FE040 Dengue Virus, Zika Virus da Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Zazzabin Dengue (DF), wanda kamuwa da cutar dengue (DENV) ke haifarwa, yana ɗaya daga cikin cututtukan da ke yaduwa na arbovirus. Matsakaicin watsa shi ya haɗa da Aedes aegypti da Aedes albopictus. DF yafi yaɗuwa a wurare masu zafi da wurare masu zafi. DENV na cikin flavivirus ne a ƙarƙashin flaviviridae, kuma ana iya rarraba shi zuwa nau'ikan serotypes 4 bisa ga antigen surface. Alamomin asibiti na kamuwa da cutar DENV sun haɗa da ciwon kai, zazzabi, rauni, haɓaka kumburin lymph, leukopenia da sauransu, da zubar jini, girgiza, rauni na hanta ko ma mutuwa a lokuta masu tsanani. A cikin 'yan shekarun nan, sauyin yanayi, ƙauyuka, saurin bunƙasa yawon shakatawa da sauran dalilai sun samar da yanayi mafi sauri da dacewa don watsawa da yaduwar DF, wanda ke haifar da yaduwar cutar ta DF.
Tashoshi
| FAM | DENV nucleic acid |
| ROX | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | -18 ℃ |
| Rayuwar rayuwa | watanni 9 |
| Nau'in Samfura | Sabbin jini |
| Ct | ≤38 |
| CV | ＜5% |
| LoD | 500 Kwafi/ml |
| Musamman | Sakamakon gwajin tsangwama ya nuna cewa lokacin da adadin bilirubin a cikin jini bai wuce 168.2μmol/ml ba, yawan haemoglobin da ake samu ta hanyar hemolysis bai wuce 130g/L ba, adadin lipid na jini bai wuce 65mmol/ml ba, jimillar IgG a cikin jini bai wuce 5mg/mL ba, babu wani tasiri a kan kwayar cutar dengue ko kwayar cutar Zikun. Kwayar cutar Hepatitis A, Hepatitis B Virus, Hepatitis C Virus, Herpes Virus, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile virus da kuma jinin jikin dan adam an zabo don gwajin amsawa, kuma sakamakon ya nuna cewa babu wani abu tsakanin wannan kit da cututtukan da aka ambata a sama. |
| Abubuwan da ake Aiwatar da su | Aiwatar da Tsarin Halitta 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya LightCycler®480 Real-Time PCR tsarin LineGene 9600 Plus Tsarin Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Zabin 1.
TIANAmp Virus DNA/RNA Kit (YDP315-R), kuma hakar ya kamata a gudanar da shi daidai da umarnin don amfani. Girman samfurin da aka fitar shine 140μL, kuma ƙimar da aka ba da shawarar shine 60μL.
Zabin 2.
Macro & Micro-Test Janar DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za a iya amfani da tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C) da MacroB06C, Micro-Test Med-Tech Co., Ltd., da kuma hakar ya kamata a gudanar bisa ga umarnin don amfani. Girman samfurin da aka fitar shine 200μL, kuma ƙimar da aka ba da shawarar shine 80μL.
