Enterovirus 71 (EV71)
Sunan samfur
HWTS-EV003- Enterovirus 71 (EV71) Kayan Gane Acid Nucleic Acid (Fluorescence PCR)
Epidemiology
Ciwon ƙafar-bakin hannu cuta ce mai yaduwa ta hanyar enteroviruses (EV).A halin yanzu, an sami nau'ikan serotypes na enteroviruses guda 108, waɗanda suka kasu kashi huɗu: A, B, C da D. Daga cikinsu, enterovirus EV71 da CoxA16 sune manyan ƙwayoyin cuta.Cutar ta fi faruwa a cikin yara 'yan kasa da shekaru 5, kuma tana iya haifar da cutar sankara a hannu, ƙafafu, baki da sauran sassa.Ƙananan yara za su haifar da rikitarwa irin su myocarditis, edema na huhu, da aseptic meningoencephalitis.
Tashoshi
| FAM | Farashin EV71 |
| ROX | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 9 |
| Nau'in Samfura | Oropharyngeal swab,Herpes ruwa |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500 Kwafi/ml |
| Abubuwan da ake Aiwatar da su | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Tsarin PCR na Gaskiya LineGene 9600 Plus Tsarukan Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Zabin 1.
Shawarar hakar reagent: Macro & Micro-Test Sample Reagent Reagent (HWTS-3005-8), da hakar ya kamata a gudanar bisa ga umarnin don amfani.Samfuran da aka fitar sune swabs na oropharyngeal ko samfuran ruwan herpes daga marasa lafiya waɗanda aka tattara akan wurin.Ƙara swabs ɗin da aka tattara a cikin Macro & Micro-Test Samfur Release Reagent (HWTS-3005-8) kai tsaye, vortex kuma gauraya da kyau, sanya a dakin da zafin jiki na minti 5, fitar da su sannan a juya don haɗuwa da kyau, don samun RNA. na kowane samfurin.
Zabin 2.
Shawarar hakar reagent: Macro & Micro-Test Janar DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za a iya amfani da tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., da hakar ya kamata a gudanar bisa ga umarnin don amfani.Ƙarfin samfurin da aka fitar shine 200µL, kuma ƙimar da aka ba da shawarar shine 80µL.
Zabin3.
Shawarar fitar da reagent: QIAamp Viral RNA Mini Kit (52904) ta QIAGEN ko TIANAmp Virus DNA/RNA Kit (YDP315-R), kuma hakar ya kamata a gudanar da shi daidai da umarnin don amfani.Girman samfurin da aka fitar shine 140μL, kuma ƙimar da aka ba da shawarar shine 60µL.
