Enterovirus 71 (EV71)
Sunan samfurin
Kayan Gano Acid na Nucleic (Fluorescence PCR) na HWTS-EV003- Enterovirus 71 (EV71)
Ilimin Cututtuka
Cutar hannu da ƙafa da baki cuta ce mai yaɗuwa wadda ƙwayoyin cuta masu yaɗuwa ke haifarwa (EV). A halin yanzu, an gano nau'ikan ƙwayoyin cuta iri 108, waɗanda aka raba zuwa ƙungiyoyi huɗu: A, B, C da D. Daga cikinsu, ƙwayoyin cuta masu yaɗuwa EV71 da CoxA16 sune manyan ƙwayoyin cuta. Cutar galibi tana faruwa ne a cikin yara 'yan ƙasa da shekara 5, kuma tana iya haifar da herpes a hannuwa, ƙafafu, baki da sauran sassa. Ƙananan yara za su kamu da matsaloli kamar myocarditis, kumburin huhu, da kuma meningoencephalitis.
Tashar
| FAM | EV71 |
| ROX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 9 |
| Nau'in Samfuri | Tafin hanci,Ruwan herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | Kwafi 500/mL |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 QuantStudio®Tsarin PCR guda 5 na Ainihin Lokaci Tsarin PCR na SLAN-96P na Gaskiya LightCycler®Tsarin PCR na Ainihin Lokaci 480 Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokaci Mai Keke Mai Zafi na MA-6000 na Ainihin Lokaci Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Zaɓi na 1.
Maganin cirewa da aka ba da shawarar: Maganin sake fitar da samfurin Macro & Micro-Test (HWTS-3005-8), kuma ya kamata a gudanar da cirewar bisa ga umarnin amfani. Samfuran da aka cire sune swabs na oropharyngeal ko samfuran ruwan herpes daga marasa lafiya da aka tattara a wurin. Sai a zuba swabs ɗin da aka tattara a cikin Maganin sake fitar da samfurin Macro & Micro-Test (HWTS-3005-8) kai tsaye, a juya a gauraya sosai, a sanya a zafin ɗaki na minti 5, a fitar da su sannan a juya su don su gauraya sosai, don samun RNA na kowane samfurin.
Zaɓi na 2.
Shawarar da aka ba da shawarar cirewa: Kit ɗin DNA/RNA na Macro & Micro-Test General (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za a iya amfani da shi tare da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., kuma ya kamata a gudanar da cirewar bisa ga umarnin amfani. Girman samfurin da aka cire shine 200µL, kuma girman fitarwa da aka ba da shawarar shine 80µL.
Zaɓi na 3.
Shawarar cirewa: Kit ɗin QIAamp Viral RNA Mini Kit (52904) ta QIAGEN ko Kit ɗin TIAnamp Virus DNA/RNA (YDP315-R), kuma ya kamata a gudanar da cirewar bisa ga umarnin amfani. Girman samfurin da aka cire shine 140μL, kuma girman fitarwa da aka ba da shawarar shine 60µL.
