Enterovirus 71 (EV71)
Sunan samfur
HWTS-EV003- Enterovirus 71 (EV71) Kayan Gane Acid Nucleic Acid (Fluorescence PCR)
Epidemiology
Ciwon ƙafar-bakin hannu cuta ce mai yaduwa ta hanyar enteroviruses (EV). A halin yanzu, an gano nau'ikan serotypes na enteroviruses guda 108, waɗanda suka kasu kashi huɗu: A, B, C da D. Daga cikinsu, enterovirus EV71 da CoxA16 sune manyan ƙwayoyin cuta. Cutar ta fi faruwa a cikin yara 'yan kasa da shekaru 5, kuma tana iya haifar da cutar sankara a hannu, ƙafafu, baki da sauran sassa. Ƙananan yara za su haifar da rikitarwa irin su myocarditis, edema na huhu, da aseptic meningoencephalitis.
Tashoshi
| FAM | Farashin EV71 |
| ROX | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 9 |
| Nau'in Samfura | Oropharyngeal swab,Herpes ruwa |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500 Kwafi/ml |
| Abubuwan da ake Aiwatar da su | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Tsarin PCR na Gaskiya LineGene 9600 Plus Tsarukan Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Zabin 1.
Shawarar hakar reagent: Macro & Micro-Test Sample Reagent Reagent (HWTS-3005-8), da hakar ya kamata a gudanar bisa ga umarnin don amfani. Samfuran da aka fitar sune swabs na oropharyngeal ko samfuran ruwan herpes daga marasa lafiya waɗanda aka tattara akan wurin. Ƙara swabs ɗin da aka tattara a cikin Macro & Micro-Test Samfur Release Reagent (HWTS-3005-8) kai tsaye, vortex da haɗuwa da kyau, sanya a dakin da zafin jiki na minti 5, fitar da su sannan a juya don haɗuwa da kyau, don samun RNA na kowane samfurin.
Zabin 2.
Shawarar hakar reagent: Macro & Micro-Test Janar DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za a iya amfani da Macro & Micro-Test Atomatik Nucleic Acid Extractor) 30-30CW by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., kuma hakar ya kamata a gudanar bisa ga umarnin don amfani. Girman samfurin da aka fitar shine 200µL, kuma ƙarar da aka ba da shawarar shine 80µL.
Zabin3.
Shawarar fitar da reagent: QIAamp Viral RNA Mini Kit (52904) ta QIAGEN ko TIANAmp Virus DNA/RNA Kit (YDP315-R), kuma hakar ya kamata a gudanar da shi daidai da umarnin amfani. Girman samfurin da aka fitar shine 140μL, kuma ƙimar da aka ba da shawarar shine 60µL.
