Enterovirus Universal, EV71 da CoxA16
Sunan Samfuri
Kayan Gano Acid na Nucleic na HWTS-EV026B-Enterovirus Universal, EV71 da CoxA16 (Fluorescence PCR)
HWTS-EV020Y/Z- Kayan Gano Acid na Nucleic da aka busar da shi ta hanyar daskare (Fluorescence PCR)
Takardar Shaidar
CE/MDA(HWTS-EV026)
Ilimin Cututtuka
Cutar Hannu da Baki (HFMD) cuta ce da ta zama ruwan dare gama gari a cikin yara. Yawanci tana faruwa ne a cikin yara 'yan ƙasa da shekara 5, kuma tana iya haifar da herpes a hannuwa, ƙafafu, baki da sauran sassan jiki, kuma ƙaramin adadin yara na iya haifar da matsaloli kamar su myocarditis, kumburin huhu, meningoencephalitis, da sauransu. Yaran da ke fama da cututtuka masu tsanani suna lalacewa cikin sauri kuma suna iya mutuwa idan ba a yi musu magani da wuri ba.
A halin yanzu, an gano nau'ikan ƙwayoyin cuta guda 108, waɗanda aka raba zuwa ƙungiyoyi huɗu: A, B, C da D. Kwayoyin cuta masu haifar da HFMD sun bambanta, amma enterovirus 71 (EV71) da coxsackievirus A16 (CoxA16) sune suka fi yawa kuma ban da HFMD, suna iya haifar da matsaloli masu tsanani na tsarin jijiyoyi na tsakiya kamar meningitis, encephalitis, da kuma gurguwar jini mai tsanani.
Tashar
| FAM | Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Ikon ciki |
Sigogi na Fasaha
| Ajiya | Ruwa: ≤-18℃ A cikin duhuRashin daidaituwar yanayi: ≤30℃ |
| Tsawon lokacin shiryawa | Ruwa: Watanni 9Rushewar ƙwayoyin halitta: watanni 12 |
| Nau'in Samfuri | Samfurin swab na makogwaro, ruwan herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Kwafi 500/mL |
| Kayan Aiki Masu Amfani | Zai iya daidaita manyan kayan aikin PCR masu haske a kasuwa.Tsarin PCR na ABI 7500 na Gaskiya Tsarin PCR na ABI 7500 Mai Sauri a Lokaci-lokaci Tsarin PCR na SLAN-96P na Gaskiya Tsarin PCR na Ainihin Lokaci na QuantStudio®5 Tsarin PCR na LightCycler®480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokaci Mai Keke Mai Zafi na MA-6000 na Ainihin Lokaci Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Maganin PCR na Jimla
Shawarar da aka ba da shawarar cirewa: Kit ɗin Macro & Micro-Test Viral DNA/RNA (HWTS-3017) (wanda za a iya amfani da shi tare da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ya kamata a yi cirewar bisa ga littafin umarnin. Girman samfurin da aka cire shine 200μL, kuma girman fitarwa da aka ba da shawarar shine 80μL.
Maganin cirewa da aka ba da shawarar: Maganin sake fitar da samfurin Macro & Micro-Test (HWTS-3005-8). Ya kamata a yi cirewar bisa ga littafin umarnin. Samfuran cirewar sune swabs na oropharyngeal ko samfuran ruwan herpes na marasa lafiya waɗanda aka tattara a wurin. Sai a zuba swabs ɗin da aka tattara kai tsaye a cikin Maganin sake fitar da samfurin Macro & Micro-Test, a gauraya sosai, a sanya a zafin ɗaki na minti 5, a cire sannan a juya a gauraya sosai don samun RNA na kowane samfurin.
Shawarar cirewa: QIAamp Viral RNA Mini Kit (52904) ta QIAGEN ko Nucleic Acid Extraction ko Purification Reagent (YDP315-R). Ya kamata a yi cirewar bisa ga umarnin da aka bayar.
