Enterovirus Universal, EV71 da CoxA16
Sunan samfur
HWTS-EV026B-Enterovirus Universal, EV71 da CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
HWTS-EV020Y/Z-Freeze-bushe Enterovirus Universal, EV71 da CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
Takaddun shaida
CE/MDA (HWTS-EV026)
Epidemiology
Ciwon-bakin hannu (HFMD) cuta ce mai saurin yaduwa a cikin yara. Yawanci yana faruwa ne a yara 'yan kasa da shekaru 5, kuma yana iya haifar da ciwon huhu a hannu, ƙafafu, baki da sauran sassa, kuma ƙananan yara na iya haifar da rikice-rikice irin su myocarditis, edema na huhu, aseptic meningoencephalitis, da dai sauransu. Yaran da ke fama da cututtuka masu tsanani suna lalacewa da sauri kuma suna da wuyar mutuwa idan ba a yi musu gaggawa ba.
A halin yanzu, an gano serotypes 108 na enteroviruses, waɗanda aka kasu kashi huɗu: A, B, C da D. Enteroviruses da ke haifar da HFMD iri-iri ne, amma enterovirus 71 (EV71) da coxsackievirus A16 (CoxA16) sune mafi yawan na kowa kuma ban da HFMD, na iya haifar da mummunar cututtuka na tsakiya, cututtuka masu tsanani, cututtuka irin su flaxitis.
Tashoshi
| FAM | Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | Farashin EV71 |
| CY5 | Ikon cikin gida |
Ma'aunin Fasaha
| Adana | Liquid: ≤-18 ℃ A cikin duhuLyophilization: ≤30 ℃ |
| Rayuwar rayuwa | Ruwa: watanni 9Lyophilization: watanni 12 |
| Nau'in Samfura | Samfurin swab na makogwaro, ruwan Herpes |
| Ct | ≤38 |
| CV | ≤5.0 da |
| LoD | 500 Kwafi/ml |
| Abubuwan da ake Aiwatar da su | Zai iya dacewa da kayan aikin PCR na yau da kullun akan kasuwa.ABI 7500 Tsarin PCR na Gaskiya Tsarin PCR na ABI 7500 Mai Saurin Gaskiya SLAN-96P Tsarin PCR na Gaskiya QuantStudio®5 Tsarin PCR na Gaskiya LightCycler®480 Tsarin PCR na Gaskiya LineGene 9600 Plus Tsarukan Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Jimlar Magani na PCR
Shawarar hakar reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (wanda za a iya amfani da tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. The hakar ya kamata a za'ayi bisa ga. Girman samfurin da aka fitar shine 200μL, kuma ƙimar da aka ba da shawarar shine 80μL.
Nasihar hakar reagent: Macro & Micro-Test Samfurin Sakin Reagent (HWTS-3005-8). Ya kamata a aiwatar da hakar bisa ga jagorar koyarwa. Samfurin cirewa sune swabs na oropharyngeal ko samfuran ruwan herpes na marasa lafiya waɗanda aka tattara akan wurin. Ƙara swabs ɗin da aka tattara kai tsaye zuwa Macro & Micro-Test Samfurin Sakin Reagent, vortex da gauraya da kyau, sanya a dakin da zafin jiki na minti 5, fitar sannan a juya a gauraya da kyau don samun RNA na kowane samfurin.
Shawarar fitar da reagent: QIAamp Viral RNA Mini Kit (52904) ta QIAGEN ko Nucleic Acid Extraction ko Reagent Tsarkake (YDP315-R). Ya kamata a aiwatar da hakar bisa ga umarnin umarnin.
