Kwayar Cutar Encephalitis ta Forest
Sunan samfurin
Kayan Gano Acid na Nucleic na Kwayoyin cuta na HWTS-FE006 (Fluorescence PCR)
Ilimin Cututtuka
Ciwon daji (Fe), wanda aka fi sani da ciwon daji (Tick-borne encephalitis, TBE), cuta ce mai saurin yaɗuwa a cikin tsarin jijiyoyi na tsakiya wanda ƙwayar cuta ta daji ke haifarwa. Kwayar cutar daji (Forest encephalitis) ta kasance cikin dangin Flavivirus na dangin Flaviviridae. Ƙwayoyin cutar suna da siffar zagaye tare da diamita na 40-50nm. Nauyin ƙwayoyin halitta shine kusan 4×10.6Da, kuma kwayar halittar kwayar cuta RNA ce mai ma'ana mai kyau, mai rabe-rabe guda ɗaya.[1]A asibiti, ana iya gane shi da zazzabi mai tsanani, ciwon kai, suma, saurin kamuwa da ciwon meningeal, da gurguwar tsoka a wuya da gaɓoɓi, kuma yana da yawan mace-mace mai yawa. Gano cutar daji da wuri da sauri shine mabuɗin maganin cutar daji, kuma kafa hanyar ganewar asali mai sauƙi, takamaiman kuma mai sauri yana da matuƙar muhimmanci a cikin ganewar cutar daji ta daji.[1,2].
Tashar
| FAM | ƙwayar cutar daji ta encephalitis ta nucleic acid |
| ROX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 9 |
| Nau'in Samfuri | sabon magani |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Kwafi 500/mL |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 QuantStudio®Tsarin PCR guda 5 na Ainihin Lokaci Tsarin PCR na SLAN-96P na Gaskiya LightCycler®Tsarin PCR na 480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokaci Mai Keke Mai Zafi na MA-6000 na Ainihin Lokaci Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Shawarar da aka ba da shawarar cirewa: Kayan Hana Tsarkakewa ko Nucleic Acid (YDP315-R) na Tiangen Biotech(Beijing) Co., Ltd., ya kamata a gudanar da cirewar bisa ga umarnin da aka bayar. Yawan samfurin da aka fitar da shawarar shine 140μL kuma girman fitar da ruwa da aka ba da shawarar shine 60μL.
Shawarar cirewa: Kit ɗin DNA/RNA na Macro & Micro-Test General (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006, HWTS-3006C, HWTS-3006B). Ya kamata a gudanar da cirewar bisa ga umarnin da aka bayar. Girman samfurin da aka fitar da shawarar shine 200μL kuma girman fitarwa da aka ba da shawarar shine 80μL.
