Encessis na kabad
Sunan Samfuta
Hwts-fee006 daji fress
TopideMology
Kurfin daji na daji (fe), wanda kuma aka sani da kashin-borne encephalitis (tbe encephalitis, tbe), cuta ce ta hanzari ta tsarin rashin damuwa da aka haifar. Kwayar daji ta daji ta kasance tana da halittar dan kasar Flavivirie iyali. Abubuwan da cutar ƙwayar cuta suna da fecherical tare da diamita na 40-50nm. Nauyin kwayar halitta shine kusan 4 × 106Da, da kwayar cutar ta hakika ce mai ma'ana, RNA guda biyu[1]. Aure, ana santa da zazzabi mai tsananin sanyi, ciwon kai, coma, saurin ƙwayar mani, da ƙwayoyin tsoka, kuma yana da yawan mace-mace. Cutar da wuri da sauri da saurin kamuwa da cuta ita ce mabuɗin don maganin cututtukan daji na daji, da kuma kafa ingantacciyar hanyar ganewar asali, da kuma takamaiman tsari a cikin cutar asibiti na ciwan daji na tsirara[1,2].
Hanya
| Famfo | Kurfuwar kabad na daji |
| Rox | Kula da ciki |
Sigogi na fasaha
| Ajiya | ≤-18 ℃ |
| Shelf-rayuwa | Watanni 9 |
| Nau'in samfur | sabo ne serum |
| Tt | ≤38 |
| CV | ≤5.0% |
| Lod | 500Copies / ml |
| Kayan aiki | Amfani da biosystems 7500 Tsarin aiki na lokaci-lokaci Amfani da biosystems 7500 mai sauri na lokaci-lokaci tsarin tsari Quicstatawa®5 Tsarin PCR na ainihi Slan-96p Real-Lokaci na Real-Lokaci Laima®Tsarin PCR na Real-Lokaci Layin 9600 da ƙarin bayanan ganowa na yau da kullun PCR Ma-6000 na lokaci-lokaci na zamani Tsarin cfx96 na lokaci-lokaci na lokaci-lokaci Biorad cfx opus 96 real-lokaci-lokaci tsarin PCR |
Aiki kwarara
Mai ba da shawarar hakar da aka ba da shawarar: hakar nucleic acid ko kayan aikin tsarkakewa (YDP315-R) ta Tiahenen) Co., ltd. Theawar da aka ba da shawarar ya fitar da girma samfurin shine 140μl da shawarar ƙira ƙira shine 60μ.
Mai ba da shawarar dawo da sake dawowa: Macro & Micro-Ction kayan Janar DNA HWTS-3006, hwts-3006C, hwts-3006b). Ya kamata a gudanar da hakar bisa ga umarnin sosai. Theawar da aka ba da shawarar ya fitar da ƙara samfurin shine 200μ kuma da shawarar ƙira ƙira shine 80μL.
