Cutar Encephalitis Forest
Sunan samfur
HWTS-FE006 Kumburi Encephalitis Cutar Kwayar Kwayoyin Gano Acid (Fluorescence PCR)
Epidemiology
Encephalitis Forest (FE), wanda kuma aka sani da tick-borne encephalitis (Tick-borne encephalitis, TBE), cuta ce mai saurin yaduwa ta tsarin juyayi na tsakiya wanda kwayar cutar encephalitis na daji ke haifarwa. Kwayar cutar encephalitis na daji na cikin dangin Flavivirus na dangin Flaviviridae. Kwayoyin ƙwayoyin cuta suna da siffar zobe tare da diamita na 40-50nm. Nauyin kwayoyin yana kusan 4 × 106Da, kuma kwayar kwayar cutar kwayar halitta kyakkyawar fahimta ce, RNA mai madauri daya[1]. A asibiti, ana siffanta shi da zazzaɓi mai zafi, ciwon kai, ciwon kai, saurin kamuwa da ciwon sankarau, da gurɓacewar tsokar wuya da gaɓoɓi, kuma yana da yawan mace-mace. Farko da saurin ganewar cutar kwayar cutar dajin daji shine mabuɗin maganin ciwon daji, kuma kafa hanya mai sauƙi, ƙayyadaddun ƙayyadaddun tsarin ganewar asali da sauri yana da ma'ana mai girma a cikin binciken asibiti na ƙwayar daji.[1,2].
Tashoshi
| FAM | gandun daji encephalitis virus nucleic acid |
| ROX | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 9 |
| Nau'in Samfura | sabobin magani |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kwafi/ml |
| Abubuwan da ake Aiwatar da su | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Real-Time PCR tsarin LineGene 9600 Plus Tsarukan Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Shawarar hakar reagent: Nucleic Acid hakar ko tsarkakewa Kit (YDP315-R) ta Tiangen Biotech(Beijing) Co., Ltd., da hakar ya kamata a gudanar bisa ga umarnin sosai. Girman samfurin da aka ba da shawarar shine 140μL kuma ƙimar da aka ba da shawarar shine 60μL.
Shawarar hakar reagent: Macro & Micro-Test Janar DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-6-300) HWTS-3006B). hakar ya kamata a gudanar bisa ga umarnin sosai. Ƙarfin samfurin da aka ba da shawarar shine 200μL kuma ƙimar da aka ba da shawarar shine 80μL.
