Mutum PML-RARA Fusion Gene Mutation
Sunan samfur
Saukewa: HWTS-TM017APML-RARA Fusion Gene Mutation Kit (Fluorescence PCR)
Epidemiology
M cutar sankarar bargo (APL) wata nau'in cutar sankarar bargo ce ta musamman (AML). Kimanin kashi 95 cikin dari na marasa lafiya na APL suna tare da canji na cytogenetic na musamman, wato t (15;17) (q22;q21), wanda ya sa PML gene akan chromosome 15 da retinoic acid receptor α gene (RARA) akan chromosome 17 fused don samar da kwayar halittar PML-RARA. Saboda mabambantan raguwar kwayoyin halittar PML, za a iya raba kwayar halittar PML-RARA zuwa nau'in dogon nau'i (nau'in L), gajeriyar nau'in (nau'in S) da nau'in bambance-bambancen (nau'in V), yana lissafin kusan 55%, 40% da 5% bi da bi.
Tashoshi
| FAM | PML-RARA fusion gene |
| ROX | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | ≤-18 ℃ A cikin duhu |
| Rayuwar rayuwa | watanni 9 |
| Nau'in Samfura | kasusuwa |
| CV | <5.0 da |
| LoD | Kwafi 1000/ml. |
| Musamman | Babu wani giciye-reactivity tare da sauran fusion genes BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, da TEL-AML1 fusion genes. |
| Abubuwan da ake Aiwatar da su | Aiwatar da Tsarin Halitta 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR tsarin Tsarin Gano PCR na LineGene 9600 Plus (FQD-96A, fasahar Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Nasihar hakar reagent: RNAprep Tsabtataccen Jini Total RNA Hakar Kit (DP433). Ya kamata a gudanar da hakar bisa ga IFU.
