Canjin Halittar Haɗaɗɗen PML-RARA na ɗan adam
Sunan samfurin
HWTS-TM017AKayan Gano Canjin Halittar Haɗakar PML-RARA na Ɗan Adam (Fluorescence PCR)
Ilimin Cututtuka
Cutar sankarar bargo mai tsanani (APL) nau'in cutar sankarar bargo mai tsanani (AML) wani nau'in cutar sankarar bargo mai tsanani ne (AML). Kimanin kashi 95% na marasa lafiya na APL suna tare da wani canji na musamman na cytogenetic, wato t(15;17)(q22;q21), wanda ke sanya kwayar halittar PML akan chromosome 15 da kuma kwayar halittar retinoic acid receptor α (RARA) akan chromosome 17 don samar da kwayar halittar PML-RARA. Saboda bambance-bambancen wuraren da kwayar halittar PML ta lalace, kwayar halittar PML-RARA za a iya raba ta zuwa nau'in dogon (nau'in L), nau'in gajere (nau'in S) da nau'in bambance-bambancen (nau'in V), wanda ya kai kusan kashi 55%, 40% da 5% bi da bi.
Tashar
| FAM | Tsarin halittar PML-RARA |
| ROX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | ≤-18℃ Cikin duhu |
| Tsawon lokacin shiryawa | Watanni 9 |
| Nau'in Samfuri | bargo na ƙashi |
| CV | <5.0% |
| LoD | Kwafi 1000/mL. |
| Takamaiman Bayani | Babu wani haɗin gwiwa tsakanin kwayoyin halittar haɗin BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, da TEL-AML1. |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 QuantStudio®Tsarin PCR guda 5 na Ainihin Lokaci Tsarin PCR na SLAN-96P na Gaskiya (Hongshi Medical Technology Co., Ltd.) LightCycler®Tsarin PCR na 480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na Lokaci-lokaci (FQD-96A, fasahar Hangzhou Bioer) MA-6000 Mai Keke Mai Yawan Zafi na Ainihin Lokaci (Suzhou Molarray Co., Ltd.) Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Shawarar sinadarin cirewa: RNAprep Pure Blood Total RNA Extraction Kit (DP433). Ya kamata a gudanar da cirewar bisa ga IFU.
