Kwayar Cutar Mura A ta Duniya/H1/H3
Sunan samfurin
Kayan Gano Kwayoyin Cuta na Nucleic Acid na Duniya/H1/H3 (Fluorescence PCR)
Ilimin Cututtuka
Kwayar cutar mura tana wakiltar nau'in Orthomyxoviridae. Kwayar cuta ce da ke barazana ga lafiyar ɗan adam. Tana iya kamuwa da mai masaukin baki sosai. Barkewar yanayi tana shafar kimanin mutane miliyan 600 a duk duniya kuma tana haifar da mutuwar mutane 250,000 ~ 500,000, wanda kwayar cutar mura A ita ce babbar sanadin kamuwa da cuta da mutuwa. Kwayar cutar mura A RNA ce mai rabe ɗaya mai rabe ɗaya mai rabe ɗaya. Dangane da hemagglutinin (HA) da neuraminidase (NA), ana iya raba HA zuwa ƙananan nau'i 16, NA An raba zuwa ƙananan nau'i 9. Daga cikin ƙwayoyin cutar mura A, ƙananan nau'ikan ƙwayoyin cutar mura waɗanda za su iya kamuwa da mutane kai tsaye sune: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 da H10N8. Daga cikinsu, ƙananan nau'ikan H1 da H3 suna da matuƙar cutarwa, kuma sun cancanci a kula da su musamman.
Tashar
| FAM | kwayar cutar mura A ta duniya nau'in kwayar cuta mai suna nucleic acid |
| VIC/HEX | kwayar cutar mura ta H1 nau'in mura |
| ROX | kwayar cutar mura ta H3 nau'in mura |
| CY5 | iko na ciki |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 9 |
| Nau'in Samfuri | swab na nasopharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Kwafi 500/μL |
| Takamaiman Bayani | Babu haɗin kai tsakanin wasu samfuran numfashi kamar Mura A, Mura B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, da sauransu da kuma DNA na genomic na ɗan adam. |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 Tsarin PCR na Ainihin Lokaci na QuantStudio®5 Tsarin PCR na SLAN-96P na Gaskiya (Hongshi Medical Technology Co., Ltd.) Tsarin PCR na LightCycler®480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na Lokaci-lokaci (FQD-96A, fasahar Hangzhou Bioer) MA-6000 Mai Keke Mai Yawan Zafi na Ainihin Lokaci (Suzhou Molarray Co., Ltd.) Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Maganin Tsarkakewa ko Tsarkakewa na Nucleic Acid (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd. Ya kamata a yi cirewar bisa ga umarnin amfani. Yawan samfurin da aka cire shine 140μL, kuma girman fitar da ruwa da aka ba da shawarar shine 60μL.
Zaɓi na 2.
Kit ɗin DNA/RNA na Macro & Micro-Test Viral (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ya kamata a yi cirewar bisa ga umarnin amfani. Girman samfurin da aka cire shine 200μL, kuma girman fitarwa da aka ba da shawarar shine 80μL.
