Klebsiella Pneumoniae, Acinetobacter Baumannii da Pseudomonas Aeruginosa da kwayoyin halittar da ke jure wa magunguna (KPC, NDM, OXA48 da IMP) Multiplex
Sunan samfurin
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii da Pseudomonas Aeruginosa da kwayoyin halittar da ke jure wa magunguna (KPC, NDM, OXA48 da IMP) Kayan Gano Multiplex (Fluorescence PCR)
Takardar Shaidar
CE
Ilimin Cututtuka
Klebsiella pneumoniae cuta ce da ta zama ruwan dare gama gari a asibiti kuma ɗaya daga cikin muhimman ƙwayoyin cuta masu haifar da cututtuka da ke haifar da cututtukan nosocomial. Idan ƙarfin jiki ya ragu, ƙwayoyin cuta suna shiga huhu daga hanyar numfashi, suna haifar da kamuwa da cuta a sassa da yawa na jiki, kuma amfani da maganin rigakafi da wuri shine mabuɗin warkarwa.[1].
Mafi yawan wuraren da cutar Acinetobacter baumannii ta fi yawa su ne huhu, wanda muhimmin abu ne ga cutar pneumonia da aka samu a Asibiti (HAP), musamman cutar pneumonia da ke da alaƙa da Ventilator (VAP). Sau da yawa tana tare da wasu cututtukan ƙwayoyin cuta da fungal, tare da halaye na yawan kamuwa da cuta da kuma yawan mace-mace.
Pseudomonas aeruginosa shine mafi yawan ƙwayoyin cuta marasa gram-negative a aikin asibiti, kuma muhimmin kwayar cuta ce mai saurin kamuwa da cuta da aka samu a asibiti, tare da halaye na sauƙin mamayewa, sauƙin canzawa da juriya ga magunguna da yawa.
Tashar
| Suna | PCR-Mix 1 | PCR-Mix 2 |
| Tashar FAM | Aba | IMP |
| Tashar VIC/HEX | Sarrafa Cikin Gida | KPC |
| Tashar CY5 | PA | NDM |
| Tashar ROX | KPN | OXA48 |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | Mangwaro |
| Ct | ≤36 |
| CV | ≤10.0% |
| LoD | 1000 CFU/mL |
| Takamaiman Bayani | a) Gwajin amsawar giciye ya nuna cewa wannan kayan aikin ba shi da amsawar giciye tare da wasu cututtukan numfashi, kamar Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus da samfuran maniyyi ba tare da manufa ba, da sauransu. b) Ƙarfin hana tsangwama: Zaɓi mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, da roxithromycin, da sauransu don gwajin tsangwama, kuma sakamakon ya nuna cewa abubuwan tsangwama da aka ambata a sama ba sa tsoma baki ga gano ƙwayoyin cuta na Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa da kwayoyin halittar juriya na carbapenem KPC, NDM, OXA48 da IMP. |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 QuantStudio®Tsarin PCR guda 5 na Ainihin Lokaci LightCycler®Tsarin PCR na 480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na Lokaci-lokaci (FQD-96A, fasahar Bioer) MA-6000 Mai Keke Mai Yawan Zafi na Ainihin Lokaci (Suzhou Molarray Co., Ltd.) Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Maganin PCR na Jimla
