Klebsiella Pneumoniae, Acinetobacter Baumannii da Pseudomonas Aeruginosa da Drug Resistance Genes (KPC, NDM, OXA48 da IMP) Multiplex
Sunan samfur
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii da Pseudomonas Aeruginosa da Drug Resistance Genes (KPC, NDM, OXA48 da IMP) Multiplex Gane Kit (Fluorescence PCR)
Takaddun shaida
CE
Epidemiology
Klebsiella pneumoniae cuta ce ta yau da kullun ta asibiti kuma tana ɗaya daga cikin mahimman ƙwayoyin cuta masu haifar da cututtuka na nosocomial.Lokacin da juriya na jiki ya ragu, ƙwayoyin cuta suna shiga cikin huhu daga hanyar numfashi, suna haifar da cututtuka a sassa da yawa na jiki, kuma fara amfani da maganin rigakafi shine mabuɗin magani.[1].
Wurin da aka fi samun kamuwa da cutar Acinetobacter baumannii shine huhu, wanda shine muhimmin ƙwayar cuta ga Asibiti da aka samu ciwon huhu (HAP), musamman Ventilator related pneumonia (VAP).Sau da yawa yana tare da wasu cututtuka na ƙwayoyin cuta da na fungal, tare da halaye na yawan ƙwayar cuta da yawan mace-mace.
Pseudomonas aeruginosa shine mafi yawan bacilli marasa fermentative gram-negative bacilli a cikin aikin asibiti, kuma yana da mahimmancin ƙwayar cuta don kamuwa da cuta ta asibiti, tare da halayen mulkin mallaka mai sauƙi, sauƙi mai sauƙi da juriya na ƙwayoyi masu yawa.
Tashoshi
| Suna | PCR-Mix 1 | PCR-Mix 2 |
| Tashar FAM | Aba | IMP |
| Tashar VIC/HEX | Ikon Cikin Gida | KPC |
| CY5 Channel | PA | NDM |
| Tashar ROX | KPN | OXA48 |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | Sputum |
| Ct | ≤36 |
| CV | ≤10.0% |
| LoD | 1000 CFU/ml |
| Musamman | a) Gwajin giciye-reactivity ya nuna cewa wannan kit ɗin ba shi da amsawar giciye tare da sauran ƙwayoyin cuta na numfashi, irin su Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus mura, Acinetobacter jelly, Acinetobacter, Lemonophilia, Peaschemonymolytic, Acinetobacter. fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus da sputum samfurori ba tare da hari ba, da dai sauransu. b) Ƙarfin tsangwama: Zaɓi mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, da roxithromycin, da dai sauransu don gwajin tsangwama, kuma sakamakon ya nuna cewa abubuwan da aka ambata a sama. kada ku tsoma baki tare da gano Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa da carbapenem juriya kwayoyin KPC, NDM, OXA48 da IMP. |
| Abubuwan da ake Aiwatar da su | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya LightCycler®480 Real-Time PCR tsarin Tsarin Gano PCR na LineGene 9600 Plus (FQD-96A, fasahar Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Jimlar Magani na PCR
