Cutar Numfashi ta Gabas ta Tsakiya Coronavirus Nucleic Acid
Sunan samfur
HWTS-RT031A-Tsakiya Tsakanin Ciwon Hankali Ciwon Cutar Coronavirus Makamin Gano Acid Nucleic (Fluorescent PCR)
Epidemiology
Cutar numfashi ta Gabas ta Tsakiya coronavirus (MERS-CoV), β-coronavirus da ke haifar da itaAn fara gano cutar ta numfashi a cikin mutane, an fara gano shi a cikin wani magidanci mai shekaru 60 dan kasar Saudi Arabiya mai haƙuri a ranar 24 ga Yuli, 2012. Gabatarwar asibiti na kamuwa da cutar MERS-CoV ya fito ne daga yanayin asymptomatic ko ƙananan alamun numfashi zuwa mummunan cututtukan numfashi har ma da mutuwa.
Tashoshi
FAM | MERS cutar RNA |
VIC(HEX) | Ikon Cikin Gida |
Ma'aunin Fasaha
Adana | ≤-18℃ A cikin duhu |
Rayuwar rayuwa | watanni 9 |
Nau'in Samfura | An tattara sabo-sabo na nasopharyngeal swabs |
CV | ≤5.0% |
Ct | ≤38 |
LoD | Kwafi 1000/ml |
Musamman | Babu wani haɗin kai tare da coronaviruses ɗan adam coronavirus SARSr-CoV da sauran cututtukan gama gari. |
Kayayyakin aiki: | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Tsarin PCR na Gaskiya LineGene 9600 Plus Tsarin Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Zabin 1.
Shawarar hakar reagent: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction ko Purification Reagent (YDP315-R) ta Tiangen Biotech (Beijing) Co., Ltd.
Zabin 2.
Nasihar hakar reagent: Macro & Micro-Test Janar DNA/RNA Kit (HWTS-3017) da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).