Gwajin EasyAmp ta Macro & Micro—- Kayan Aikin Ƙarfafa Hasken Hasken Hasken Isothermal Mai Ɗaukewa Mai Dace da LAMP/RPA/NASBA/HDA

Kyakkyawan Aiki & Faɗin Aikace-aikace

Easy Amp, ta hanyar fasahar iƘara yawan sinadarin sothermal nucleic acidyana da siffofi masu ingancihankali da kumagajeremartanilokacin ba tare da buƙatu ba tsarin canza yanayin zafi. Saboda haka, yana dafitoedkamar yaddamafifasahar da aka fi so don gwajin ƙwayoyin nucleic acid cikin sauri.Ba tare da rikitarwa batsarin hawan keke na hermal, na'urar ƙara ƙarfin isothermal mai sauƙi da šaukuwaiya gamsar da bukatuncikakken tsarin amsawa.

Macro & Micro-Test sun haɓaka kansu da kansukuma an ƙaddamarEasyAmp,kayan aikin ƙara haske mai haske mai iya ɗaukadon aikace-aikace daban-daban.

A halin yanzu,Sauƙin Amp an tabbatar da cewa anya dace da dabarun ƙara ƙarfin isothermal da dama: Ƙara ƙarfin Isothermal Mai Matsakaici (LAMP), Ƙara ƙarfin Recombinase Polymerase (RPA/RAA/MIRA), Ƙara ƙarfin Nucleic Acid Mai Ba da Tsarin Nucleic Acid (NASBA), da Ƙara ƙarfin Helicase Mai Dogara (HDA)Zai zama wani kayan aiki na zamani don gwajin ƙwayoyin nucleic acid cikin sauri tare da amfani mai faɗi.

· Ƙarfafawar Isothermal Mai Matsakaici (LAMP):Ƙarawa a60-65°Cda dukamartanitsarian kammala cikin mintuna 30.Duba Hoto na 1 don faɗaɗa shiƙa'ida:

 

Hoto na 1. Ka'idaof Fitila 

· Haɗa Ƙarfafa ...Ƙarawa a37°C-42°Cda dukamartanitsarian kammala cikin mintuna 10-30.Duba Hoto na 2 don ganin taƙa'idar faɗaɗawa:

Hoto na 2. Ka'idaof Ƙara Ƙarfafa Polymerase na Recombinase

·Ƙara Tsarin Nucleic Acid (NASBA):Ƙarawa a42°Cda dukamartanitsarian kammala shi cikin mintuna 40-120.Duba Hoto na 3 don ganin taƙa'idar faɗaɗawa:

Hoto na 3. Ka'idaof Ƙarawa Bisa Tsarin Nucleic Acid

· Ƙara ƙarfin Helicase (HDA):Ƙarawa a65°Cda dukamartanitsarian kammala shi cikin mintuna 70-90.Duba Hoto na 4 don ganin taƙa'idar faɗaɗawa:

Hoto na 4. Ka'idaof Ƙarawa Mai Dogara da Helicase

· EasyAmpkunnaIngantaccen haɓakawa mai inganci da kwanciyar hankali don gano DNA da RNA ta amfani da dabarun isothermal guda huɗu da aka ambata a sama. Yana samun ƙarfin haɓaka haɓakawa har zuwa kwafi 200/mL kuma yana tabbatar da babban takamaiman aiki ba tare da amsawar giciye a cikin haɓaka manufa ba.(Ian kwatanta shi a cikin Figures 5 zuwa 7).

Gano RNA na Gano DNA

Hoto na 5. Dandalin RAA/MIRA

Gano RNA na Gano DNA

Hoto na 6. Dandalin LAMP

 

Gano RNA na Gano DNA

Hoto na 7. Dandalin NASBA

Amfanin Samfuri:

Mai Sauri

· EPIAfasaha ta fahimcisakamako mai kyauan gano a cikin minti 5.

· Sauƙin aiki tare da cirewa mataki ɗayadon faɗaɗawa akan kayan aiki.

Inganci

· Kayayyaki 4 masu zaman kansu don gwaji akan buƙata, gwaji a lokaci guda na 4 daban-dabannau'in gwajis.

· Kowace na'ura mai iya yin nazarin samfura huɗu a lokaci guda.

· Biyu/Huɗufluorescencetashoshidon gano abubuwa da yawa masu yawa.

Daidaituwa

· Mai dacewa dada yawadandamalin isothermal kamar LAMP, RPA, NASBA, HDA.

· An ƙera shi don ƙara yawan zafin jiki bayan an yi amfani da na'urar jujjuyawa mai ƙarancin zafi.

· Keɓanceiyadon biyan takamaiman buƙatun abokin ciniki.

Mai Sauƙin Amfani

· Pƙirar da za a iya amfani da ita a kan tebur(290×245×128mm, 2kg) don aikace-aikace daban-daban na senarios.

· Taɓawa mai launi 7-inchdon sauƙin aiki da kuma kyakkyawan sakamako.

· Firintar zafi da aka gina a ciki don buga sakamako nan take.

· Na'urar daukar hoto mai hadewa don karanta abubuwan ganowa ta atomatik.