Nau'o'i 17 na HPV (16/18/6/11/44 Bugawa)
Sunan samfur
HWTS-CC015 17 Nau'o'in Mutum Papillomavirus (16/18/6/11/44 Bugawa) Kayan Gane Acid Nucleic (Fluorescence PCR)
Epidemiology
Ciwon daji na mahaifa yana daya daga cikin ciwace-ciwacen da aka fi sani a cikin mahaifar mace.An nuna cewa kamuwa da cutar ta HPV mai dawwama da cututtuka masu yawa na ɗaya daga cikin manyan abubuwan da ke haifar da kansar mahaifa.A halin yanzu har yanzu akwai rashin ingantaccen jiyya da aka yarda da su don cutar kansar mahaifa ta HPV.Don haka, ganowa da wuri da rigakafin kamuwa da cutar sankarar mahaifa da HPV ke haifarwa shine mabuɗin rigakafin cutar kansar mahaifa.Ƙirƙirar gwaje-gwaje masu sauƙi, ƙayyadaddun ƙayyadaddun gwaje-gwaje na gaggawa don ƙwayoyin cuta yana da mahimmanci ga ganewar asibiti na ciwon daji na mahaifa.
Tashoshi
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Ikon cikin gida |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Ikon cikin gida |
Ma'aunin Fasaha
| Adana | -18 ℃ |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | Samfurin fitsari, samfurin swab na mahaifa, samfurin swab na mace |
| Ct | ≤28 |
| LoD | 300 Kwafi/ml |
| Musamman | Babu wani giciye-reactivity tare da Ureaplasma urealyticum, Chlamydia trachomatis na haifuwa fili, Candida albicans, Neisseria gonorrheae, Trichomonas vaginalis, Mold , Gardnerella da sauran HPV iri ba rufe da kit. |
| Abubuwan da ake Aiwatar da su | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Tsarin PCR na Gaskiya LineGene 9600 Plus Tsarukan Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Shawarar hakar reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za a iya amfani da tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ƙara 200μL na al'ada saline don sake dakatar da pellet a mataki na 2.1, sannan kuma za a gudanar da hakar bisa ga umarnin. zuwa umarnin don amfani da wannan reagent hakar.Adadin da aka ba da shawarar shine 80μL.
Nasihar hakar reagent: QIAamp DNA Mini Kit (51304) ko Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Ƙara 200μL na saline na al'ada don sake dakatar da pellet a mataki na 2.1, sannan ya kamata a gudanar da hakar bisa ga umarnin don amfani da wannan reagent na hakar.Girman samfurin da aka fitar na samfurori duk 200μL ne, kuma ƙimar da aka ba da shawarar shine 100μL.
Nasihar hakar reagent: Macro & Micro-Test Samfurin Sakin Reagent (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L).Ƙara 200μL na samfurin sakewa don sake dakatar da pellet a mataki na 2.1, sannan ya kamata a gudanar da hakar bisa ga umarnin don amfani da wannan reagent na hakar.
