Nau'o'i 17 na HPV (Rubutu 16/18/6/11/44)
Sunan samfurin
HWTS-CC015 Nau'ikan Kwayar cutar Papilloma ta ɗan adam guda 17 (16/18/6/11/44 Rubutawa) Kayan Gano Acid na Nucleic (Fluorescence PCR)
Ilimin Cututtuka
Ciwon daji na mahaifa yana ɗaya daga cikin ciwace-ciwacen da suka fi yawa a cikin mata. An nuna cewa kamuwa da cutar HPV mai ɗorewa da kamuwa da cuta da yawa suna ɗaya daga cikin manyan abubuwan da ke haifar da ciwon daji na mahaifa. A halin yanzu akwai rashin ingantattun magunguna da aka yarda da su gabaɗaya don ciwon daji na mahaifa wanda HPV ke haifarwa. Saboda haka, ganowa da rigakafin kamuwa da cutar da HPV ke haifarwa da wuri su ne mabuɗin rigakafin ciwon daji na mahaifa. Kafa gwaje-gwaje masu sauƙi, takamaiman kuma masu sauri don gano cututtuka yana da matuƙar mahimmanci don gano cutar kansar mahaifa a asibiti.
Tashar
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Ikon ciki |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Ikon ciki |
Sigogi na Fasaha
| Ajiya | -18℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | Samfurin fitsari, samfurin swab na mahaifa na mace, samfurin swab na farji na mace |
| Ct | ≤28 |
| LoD | Kwafi 300/mL |
| Takamaiman Bayani | Babu wani haɗin gwiwa na amsawa ga Ureaplasma urealyticum, Chlamydia trachomatis na hanyar haihuwa, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold, Gardnerella da sauran nau'ikan HPV waɗanda kayan aikin ba su rufe ba. |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 QuantStudio®Tsarin PCR guda 5 na Ainihin Lokaci Tsarin PCR na SLAN-96P na Gaskiya LightCycler®Tsarin PCR na Ainihin Lokaci 480 Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokaci Mai Keke Mai Zafi na MA-6000 na Ainihin Lokaci Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Shawarar da aka ba da shawarar cirewa: Kit ɗin Macro & Micro-Test Viral DNA/RNA (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za a iya amfani da shi tare da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ƙara 200μL na saline na yau da kullun don sake dasa pellet a mataki na 2.1, sannan a gudanar da cirewar bisa ga umarnin amfani da wannan reagent na cirewa. Girman fitarwa da aka ba da shawarar shine 80μL.
Shawarar da aka ba da shawarar cirewa: QIAamp DNA Mini Kit (51304) ko Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Ƙara 200μL na saline na yau da kullun don sake dasa pellet a mataki na 2.1, sannan a gudanar da cirewar bisa ga umarnin amfani da wannan reagent na cirewa. Yawan samfurin da aka cire duk 200μL ne, kuma girman fitarwa da aka ba da shawarar shine 100μL.
Maganin cirewa da aka ba da shawarar: Maganin sake fitar da samfurin Macro & Micro-Test (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). Ƙara 200μL na maganin sake fitar da samfurin don sake dasa pellet a mataki na 2.1, sannan a gudanar da cirewar bisa ga umarnin amfani da wannan maganin cirewa.
