Mycobacterium Tuberculosis Nucleic Acid da Rifampicin (RIF), Isoniazid Resistance (INH)

Takaitaccen Bayani:

Wannan samfurin ya dace don gano ƙimar Mycobacterium tarin fuka DNA a cikin samfuran sputum na ɗan adam a cikin vitro, da kuma maye gurbin homozygous a cikin yankin 507-533 amino acid codon (81bp, rifampicin juriya kayyade yanki) na rpoB gene wanda ke haifar da Mycobacterium tarin fuka. Rifampicin juriya.

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Cikakken Bayani

Tags samfurin

Sunan samfur

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid da Rifampicin (RIF), Isoniazid Resistance (INH) Gane Kit (Narke Curve)

Epidemiology

Mycobacterium tarin fuka, jim kadan a matsayin Tubercle bacillus (TB), ita ce kwayar cutar da ke haifar da tarin fuka.A halin yanzu, magungunan da ake amfani da su na farko na maganin tarin fuka sun hada da isoniazid, rifampicin da ethambutol, da dai sauransu. Na biyu na maganin tarin fuka sun hada da fluoroquinolones, amikacin da kanamycin, da dai sauransu. Sabbin magungunan da aka bunkasa sune linezolid, bedaquiline da delamani, da dai sauransu. Duk da haka, saboda rashin amfani da magungunan cutar tarin fuka da kuma halayen tsarin bangon tantanin halitta na mycobacterium tarin fuka, tarin fuka na mycobacterium yana haifar da juriya ga magungunan tarin fuka, wanda ke kawo kalubale mai tsanani ga rigakafi da maganin tarin fuka.

Tashoshi

Sunan Target			Mai rahoto	Quencher
Reaction BufferA	Reaction BufferB	Reaction BufferC	Mai rahoto	Quencher
Farashin 507-514	Farashin 513-520	IS6110	FAM	Babu
Farashin 520-527	Farashin 527-533	/	CY5	Babu
/	/	Ikon cikin gida	HEX (VIC)	Babu

Reaction BufferD	Mai rahoto	Quencher
Yankin mai tallata InhA -15C>T, -8T>A, -8T>C	FAM	Babu
KatG 315 codon 315G>A,315G>C	CY5	Babu
Yankin mai tallata AhpC -12C>T, -6G>A	ROX	Babu

Ma'aunin Fasaha

Adana	≤-18℃
Rayuwar rayuwa	watanni 12
Nau'in Samfura	sputum
CV	≤5.0%
LoD	LoD na tarin fuka na ƙasa shine 50 kwayan cuta/ml.LoD na nau'in daji mai jure rifampicin na ƙasa shine 2 × 10³kwayoyin cuta/ml, kuma LoD na nau'in mutant shine 2 × 10³kwayoyin cuta/ml.LoD na nau'in ƙwayoyin cuta na isoniazid mai jurewa shine 2x10³Bacteria/ml, kuma LoD na ƙwayoyin cuta na mutant shine 2x10³kwayoyin cuta/ml.
Musamman	Sakamakon gwajin giciye ya nuna cewa babu wani abin da ya faru a cikin gano kwayoyin halittar dan adam, da sauran cututtukan mycobacteria marasa tarin fuka da cututtukan huhu tare da wannan kayan;Ba a sami wani ra'ayi na giciye da aka gano a wuraren maye gurbi na wasu kwayoyin halitta masu jure magunguna a cikin nau'in tarin fuka na Mycobacterium na daji.
Abubuwan da ake Aiwatar da su	SLAN-96P Tsarin PCR na Gaskiya (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Tsarin PCR na Gaskiya, Fasahar Hangzhou Bioer QuantGene 9600 Tsarin PCR na Gaskiya, QuantStudio^®5 Tsarin PCR na Gaskiya.