Mycobacterium Tuberculosis Isoniazid Resistance Mutation

Takaitaccen Bayani:

Wannan kit ɗin ya dace don gano ƙimar manyan wuraren maye gurbi a cikin samfuran sputum na ɗan adam da aka tattara daga Tubercle bacillus tabbatacce marasa lafiya waɗanda ke haifar da juriya na mycobacterium tarin fuka isoniazid: yankin mai tallata InhA -15C>T, -8T>A, -8T>C;Yankin mai tallata AhpC -12C>T, -6G>A;maye gurbi na homozygous na KatG 315 codon 315G>A, 315G>C .

Aika mana imel

Cikakken Bayani

Tags samfurin

Sunan samfur

HWTS-RT137 Mycobacterium Tuberculosis Isoniazid Resistance Mutation Kit (Curve Curve)

Epidemiology

Mycobacterium tarin fuka, jim kadan a matsayin Tubercle bacillus (TB), ita ce kwayar cutar da ke haifar da tarin fuka.A halin yanzu, magungunan da ake amfani da su na farko na maganin tarin fuka sun hada da isoniazid, rifampicin da hexambutol, da dai sauransu. Na biyu na maganin tarin fuka sun hada da fluoroquinolones, amikacin da kanamycin, da dai sauransu. Sabbin magungunan da aka samar sune linezolid, bedaquiline da delamani, da dai sauransu. Duk da haka, saboda rashin amfani da magungunan cutar tarin fuka da kuma halayen tsarin bangon tantanin halitta na mycobacterium tarin fuka, tarin fuka na mycobacterium yana haifar da juriya ga magungunan tarin fuka, wanda ke kawo kalubale mai tsanani ga rigakafi da maganin tarin fuka.

Tashoshi

FAM	MP nucleic acid
ROX	Ikon Cikin Gida

Ma'aunin Fasaha

Adana	≤-18℃
Rayuwar rayuwa	watanni 12
Nau'in Samfura	sputum
CV	≤5%
LoD	Iyakar ganowa don nau'in ƙwayoyin cuta na isoniazid na daji shine 2x103 ƙwayoyin cuta/mL, kuma iyakar gano ƙwayoyin ƙwayoyin cuta shine 2x103 ƙwayoyin cuta/mL.
Musamman	a.Babu wani ra'ayi tsakanin kwayoyin halittar ɗan adam, sauran ƙwayoyin cuta na mycobacteria marasa tuberculous da cututtukan huhu da wannan kayan ya gano.b.An gano wuraren maye gurbi na wasu kwayoyin halittar da ke jure maganin miyagun ƙwayoyi a cikin nau'in cutar tarin fuka na Mycobacterium, irin su juriya da ke kayyade yanki na rifampicin rpoB gene, kuma sakamakon gwajin ya nuna babu juriya ga isoniazid, wanda ke nuni da cewa babu giciye.
Abubuwan da ake Aiwatar da su	SLAN-96P Tsarin PCR na GaskiyaBioRad CFX96 Tsarin PCR na Gaskiya LightCycler480® Tsarin PCR na Gaskiya

Gudun Aiki

Idan amfani da Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (wanda za'a iya amfani dashi tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. don hakar, ƙara 200μL na mummunan iko da samfurin sputum da aka sarrafa don gwadawa a jere, kuma ƙara 10μL na kulawar ciki daban a cikin mummunan iko, samfurin sputum da aka sarrafa don gwadawa, kuma matakan da suka biyo baya yakamata a aiwatar dasu daidai da umarnin cirewa.Adadin samfurin da aka fitar shine 200μL, kuma ƙarar da aka ba da shawarar shine 100μL.