Staphylococcus Aureus da Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)

Takaitaccen Bayani:

Ana amfani da wannan kit ɗin don gano ƙimar staphylococcus aureus da methicillin-resistant staphylococcus aureus nucleic acid a cikin samfuran sputum na ɗan adam, samfuran swab na hanci da fata da samfuran kamuwa da cuta mai laushi a cikin vitro.


Cikakken Bayani

Tags samfurin

Sunan samfur

HWTS-OT062 Staphylococcus Aureus da Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Kayan Gano Acid Nucleic (Fluorescence PCR)

Takaddun shaida

CE

Epidemiology

Staphylococcus aureus yana daya daga cikin mahimman kwayoyin cuta na kamuwa da cuta na nosocomial.Staphylococcus aureus (SA) na cikin staphylococcus ne kuma wakili ne na kwayoyin cutar Gram-positive, wanda zai iya samar da nau'i na gubobi da enzymes masu lalata.Kwayoyin cuta suna da halaye na rarrabawa mai yawa, ƙaƙƙarfan pathogenicity da babban juriya.Halin da ake iya ɗauka na nuclease (nuc) wani ƙwayar cuta ce ta staphylococcus aureus.

Tashoshi

FAM kwayoyin mecA mai jurewa methicillin
ROX

Ikon Cikin Gida

CY5 staphylococcus aureus nuc gene

Ma'aunin Fasaha

Adana ≤-18℃ & kariya daga haske
Rayuwar rayuwa watanni 12
Nau'in Samfura sputum, fata da taushi nama samfurin kamuwa da cuta, da hanci swab samfurori
Ct ≤36
CV ≤5.0%
LoD 1000 CFU/ml staphylococcus aureus, 1000 CFU/mL kwayoyin cutar methicillin.Lokacin da kit ɗin ya gano bayanin LoD na ƙasa, 1000/ml staphylococcus aureus za a iya gano shi.
Musamman Gwajin giciye-reactivity ya nuna cewa wannan kit ɗin ba shi da giciye tare da sauran ƙwayoyin cuta na numfashi kamar methicillin-sensitive staphylococcus aureus, coagulase-korau staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, pseudomonas aeruginosa, escherichia coli, pseudomonas aeruginosa. Mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae.
Abubuwan da ake Aiwatar da su Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya

QuantStudio®5 Tsarin PCR na Gaskiya

SLAN-96P Tsarin PCR na Gaskiya

LightCycler®480 Real-Time PCR tsarin

LineGene 9600 Plus Tsarin Gane PCR na Gaskiya

MA-6000 Mai ƙididdige yawan zafin jiki na ainihi

BioRad CFX96 Tsarin PCR na Gaskiya

BioRad CFX Opus 96 Tsarin PCR na Gaskiya

Gudun Aiki

Zabin 1.

Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ana iya amfani dashi tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Ƙara 200µL na saline na al'ada zuwa hazo da aka sarrafa, kuma matakan da suka biyo baya ya kamata a fitar da su bisa ga umarnin, kuma ƙarar da aka ba da shawarar shine 80µL.

Zabin 2.

Macro & Micro-Test Samfurin Sakin Reagent (HWTS-3005-8) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ƙara 1mL na Saline na al'ada zuwa hazo bayan wankewa da salin na al'ada, sannan a gauraya da kyau.Centrifuge a 13,000r/min na tsawon mintuna 5, cire maɗaukaki (ajiye 10-20µL na supernatant), kuma bi umarnin don hakar na gaba.

Shawarar hakar reagent: Nucleic Acid Extraction ko Purification Reagent (YDP302) ta Tiangen Biotech (Beijing) Co., Ltd.Ya kamata a aiwatar da hakar sosai bisa ga mataki na 2 na littafin koyarwa.Ana ba da shawarar yin amfani da RNase da ruwa maras DNase don haɓakawa tare da ƙarar 100µL.

 


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