Adenovirus Universal
Sunan samfur
HWTS-RT017A Adenovirus Kayan Gane Acid Na Duniya (Fluorescence PCR)
Epidemiology
Adenovirus ɗan adam (HAdV) na cikin kwayar halittar Mammalian adenovirus, wanda kwayar halittar DNA ce mai ɗaure biyu ba tare da ambulaf ba.Adenoviruses da aka gano ya zuwa yanzu sun haɗa da ƙungiyoyi 7 (AG) da nau'ikan 67, waɗanda 55 serotypes suna cutar da mutane.Daga cikin su, na iya haifar da cututtuka na numfashi sun hada da rukuni na B (Nau'i 3, 7, 11, 14, 16, 21, 50, 55), Rukunin C (Nau'i na 1, 2, 5, 6, 57) da rukunin E. (Nau'i na 4), kuma yana iya haifar da kamuwa da cutar gudawa na hanji shine rukunin F (Nau'i na 40 da 41) [1-8].Nau'o'i daban-daban suna da alamomin asibiti daban-daban, amma galibi cututtuka na numfashi.Cututtukan da ke haifar da cututtukan numfashi na jikin mutum sun kai 5% ~ 15% na cututtukan numfashi na duniya, da 5% -7% na cututtukan numfashi na yara na duniya[9].Adenovirus yana yaduwa a wurare da dama kuma ana iya kamuwa da ita duk tsawon shekara, musamman a wuraren da jama'a ke da yawa, wadanda ke da saurin kamuwa da cutar a cikin gida, musamman a makarantu da sansanonin sojoji.
Tashoshi
| FAM | adenovirus duniyanucleic acid |
| ROX | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | Nasopharyngeal swab,Maganin makogwaro |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 Kwafi/ml |
| Musamman | a) Gwada daidaitattun nassoshi mara kyau na kamfani ta kit, kuma sakamakon gwajin ya cika buƙatun. b) Yi amfani da wannan kit ɗin don ganowa kuma babu haɗin kai tare da sauran ƙwayoyin cuta na numfashi (kamar cutar mura A, ƙwayar cutar mura B, ƙwayar cuta ta numfashi, kwayar cutar parainfluenza, Rhinovirus, ɗan adam metapneumovirus, da sauransu) ko ƙwayoyin cuta (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, da dai sauransu). |
| Abubuwan da ake Aiwatar da su | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Tsarin PCR na Gaskiya LineGene 9600 Plus Tsarin Gane PCR na Gaskiyas (FQD-96A, HangzhouFasahar Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Tsarin PCR na Gaskiya, BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
(1) Nasihar hakar reagent:Macro & Micro-Test Samfurin Sakin Reagent (HWTS-3005-8).Ya kamata a yi hakar bisa ga umarnin.Samfurin da aka fitar shine marasa lafiya'nasopharyngeal swab ko makogwaro swab samfurori da aka tattara akan wurin.Ƙara samfuran a cikin samfurin sakewa ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex don haɗuwa da kyau, sanya a dakin da zafin jiki na minti 5, cire sannan a juye da haɗuwa da kyau don samun DNA na kowane samfurin.
(2) Nasihar hakar reagent:Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).aikin ya kamata a yi daidai da umarnin.Adadin samfurin da aka fitar shine 200μL, da kumashawarar ƙarar haɓakawais80ml ku.
(3) Nasihar hakar reagent: Nucleic Acid Extraction ko Purification Reagent (YDP)315Tiangen Biotech (Beijing) Co., Ltd., daaiki ya kamata a yi daidai da umarnin.Adadin samfurin da aka fitar shine 200μL, da kumashawarar ƙarar haɓakawais80ml ku.
