Burgedorria
Sunan Samfuta
Hwts-ot076 burgedormafi nucleic na gano kayan acid (mai kyalli PCR)
Takardar shaida
CE
TopideMology
Cutar Lyme ta haifar da kamuwa da cuta tare da ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta kuma ana watsa shi tsakanin rundunar dabbobi, tsakanin dabbobi da mutane ta hanyar ticks mai ƙarfi. Kullum kagarar cututtukan cututtukan patogen Dangane da haɓakar cututtukan hanya, ana iya raba su zuwa farkon kamuwa da cuta, tsaka-tsakin kamuwa da kamuwa da cuta, waɗanda ke da matukar wahala cutarwa ga lafiyar jama'a. Saboda haka, a cikin Cibiyar ganewar asibiti Burgdrafi, yana da matukar muhimmanci a kafa wani abu mai sauki, musamman da sauri ga gano cutar cututtukan kwari na bogralia burgdredi.
Hanya
| Famfo | DNA na bogrelia burgdrafi |
| Vic / Hex | Kula da ciki |
Sigogi na fasaha
| Ajiya | ≤-18 ℃ |
| Shelf-rayuwa | Watanni 12 |
| Nau'in samfur | Alamar jijiyoyi |
| Tt | ≤38 |
| CV | ≤5.0% |
| Lod | 500 kwafin / ml |
| Kayan aiki | Abi 7500 Tsarin aiki na lokaci-lokaci Abi 7500 mai sauri tsarin magani Quicstatawa®5 Tsarin PCR na ainihi Slan-96p Real-Lokaci na Real-Lokaci Laima®Tsarin PCR na Real-Lokaci Linegene 9600 da tsarin ganowa na yau da kullun PCR Ma-6000 na lokaci-lokaci na zamani Tsarin cfx96 na lokaci-lokaci na lokaci-lokaci Biorad cfx opus 96 real-lokaci-lokaci tsarin PCR |
Aiki kwarara
Zabi 1.
Qiaam DNA Kit da Khia (51185).It yakamata a fitar dashiA cikin tsananin aikiga koyarwar, da shawarar ƙura ƙira ita ce100μl.
Zabin 2.
JiniGenomic dnaEKit ɗin Xtramaction Kit (DP318,A'a: JingchangRikodin na'urar20210062) Ya samar da fasahar Biochem ta hanyar Tiahenan (Beijing) Co., Ltd.. It yakamata a fitar dashiA cikin tsananin aikiga koyarwar, da shawarar ƙura ƙira ita ce100μl.
Zabin 3.
Kit ɗin tsarkakakken® na maye® na tsarkakakiyar (A1120) ta Promega.It yakamata a fitar dashiA cikin tsananin aikiga koyarwar, da shawarar ƙura ƙira ita ce100μl.
