Borrelia Burgdorferi Nucleic Acid
Sunan samfurin
Kayan Gano Acid na Nucleic na HWTS-OT076 Borrelia Burgdorferi (Fluorescence PCR)
Takardar Shaidar
CE
Ilimin Cututtuka
Cutar Lyme tana faruwa ne sakamakon kamuwa da cutar Borrelia burgdorferi kuma galibi tana yaɗuwa tsakanin masu masaukin dabbobi, tsakanin dabbobi masu masaukin baki da mutane ta hanyar ƙuraje masu tauri. Cutar Borrelia burgdorferi na iya haifar da ƙaura ta Erythema chronicum ta ɗan adam, da kuma cututtuka da suka shafi tsarin jiki da yawa kamar zuciya, jijiya, da haɗin gwiwa, da sauransu, kuma bayyanar cututtuka sun bambanta. Dangane da ci gaban cututtukan, ana iya raba shi zuwa kamuwa da cuta ta farko a gida, kamuwa da cuta mai yaɗuwa a tsaka-tsaki da kuma kamuwa da cuta mai ɗorewa, waɗanda ke da mummunan illa ga lafiyar jama'a. Saboda haka, a cikin ganewar asibiti na Borrelia burgdorferi, yana da matuƙar muhimmanci a kafa hanya mai sauƙi, takamamme kuma mai sauri don gano cutar Borrelia burgdorferi.
Tashar
| FAM | DNA na Borrelia burgdorferi |
| VIC/HEX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | Samfurin jini gaba ɗaya |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Kwafi 500/mL |
| Kayan Aiki Masu Amfani | Tsarin PCR na ABI 7500 na Gaskiya Tsarin PCR na ABI 7500 Mai Sauri a Lokaci-lokaci QuantStudio®Tsarin PCR guda 5 na Ainihin Lokaci Tsarin PCR na SLAN-96P na Gaskiya LightCycler®Tsarin PCR na 480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokaci Mai Keke Mai Zafi na MA-6000 na Ainihin Lokaci Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Zaɓi na 1.
Kit ɗin Midi na Jini na QIAamp na Qiagen (51185).It ya kamata a cirebisa ga tsari mai tsauriga umarnin, kuma ƙarar fitarwa da aka ba da shawarar ita ce100μL.
Zaɓi na 2.
JiniGDNA mai yawaEkayan aikin jan hankali (DP318,A'a.: JingchangRikodin Na'ura20210062) wanda Tiangen Biochemical Technology (Beijing) Co., Ltd. suka samar.. It ya kamata a cirebisa ga tsari mai tsauriga umarnin, kuma ƙarar fitarwa da aka ba da shawarar ita ce100μL.
Zaɓi na 3.
Kayan tsarkake DNA na Wizard® (A1120) daga Promega.It ya kamata a cirebisa ga tsari mai tsauriga umarnin, kuma ƙarar fitarwa da aka ba da shawarar ita ce100μL.
