Coxsackie Virus Type A16 Nucleic Acid

Takaitaccen Bayani:

Ana amfani da wannan kit ɗin don gano ingancin ingancin in vitro na nau'in ƙwayar cuta ta Coxsackie A16 nucleic acid a cikin swabs na makogwaro na ɗan adam.


Cikakken Bayani

Tags samfurin

Sunan samfur

HWTS-EV025-Coxsackie Virus Nau'in A16 Kayan Gane Acid Nucleic Acid (Enzymatic Probe Isothermal Amplification)

Takaddun shaida

CE

Epidemiology

Wannan kit ɗin yana ɗaukar Enzymatic Probe Isothermal Amplification (EPIA), kuma yana ƙirƙira ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai da RNA tushen bincike (rProbe) don yankin da aka kiyaye sosai na Cox A16, kuma yana ƙara Bst enzyme da RNaseH enzyme a lokaci guda, wanda hagu da hagu. Ƙarshen dama na tushen RNA na rProbe suna da lakabi tare da ƙungiyoyi masu kyalli da quencher, bi da bi.Yi amfani da aikin DNA polymerase da aikin maye gurbi na Bst enzyme don haɓaka maƙasudin da za a gwada a koyaushe, enzyme RNaseH na iya raba tushen RNA akan sarkar-bincike matasan, ta yadda ƙungiyar masu kyalli da masu kashe rProbe su ne. rabu da shi fluorescing.Bugu da ƙari, za a iya amfani da guntun hagu na ragowar rProbe RNA tushe a matsayin maɗaukaki don ƙara haɓaka samar da samfur, wanda ke ƙara tara samfurin.Ana ci gaba da tara siginar mai kyalli tare da samuwar samfurin, ta haka ne aka gano ainihin acid nucleic.

Tashoshi

FAM Kwayar cutar Coxsackie A16
ROX ciki contro

Ma'aunin Fasaha

Adana

≤-18℃

Rayuwar rayuwa

watanni 12

Nau'in Samfura

Swabs na makogwaro da aka tattara sabo

CV

≤10.0%

Ct

≤38

LoD

2000 Kwafi/ml

Abubuwan da ake Aiwatar da su

Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na GaskiyaSLAN-96P Tsarin PCR na Gaskiya

LightCycler®480 Tsarin PCR na Gaskiya

Tsarin Ganewar Zazzaɓi Tsayayye na Haƙiƙan-Fluorescence Mai Sauƙi Amp HWTS1600

Gudun Aiki

Zabin 1

Shawarar hakar reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006)

Zabin 2

Nasihar hakar reagent: Macro & Micro-Test Samfurin Sakin Reagent (HWTS-3005-8).


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