Nau'o'i Takwas na Kwayoyin Cutar Numfashi
Sunan samfurin
HWTS-RT184-Nau'o'i Takwas na Kwayoyin Cuta na Nucleic Acid Kit (Fluorescence PCR)
Ilimin Cututtuka
Cutar numfashi ita ce nau'in cutar da ta fi yawa a cikin mutane, wadda za ta iya faruwa a kowace jinsi, shekaru da yanki, kuma tana ɗaya daga cikin manyan abubuwan da ke haifar da rashin lafiya da mutuwa a duniya[1]Kwayoyin cutar numfashi da ake yawan samu a asibiti sun hada da kwayar cutar mura A (IFV A), kwayar cutar mura B (IFV B), kwayar cutar syncytial ta numfashi, kwayar cutar adenovirus, kwayar cutar metapneumovirus ta mutum, kwayar cutar rhinovirus, kwayar cutar parainfluenza (I/II/III) da kwayar cutar mycoplasma pneumoniae, da sauransu.[2,3]Alamomin asibiti da alamun da kamuwa da cutar numfashi ke haifarwa suna kama da juna, amma kamuwa da cutar da cututtuka daban-daban ke haifarwa yana da hanyoyi daban-daban na magani, tasirin magani da kuma yadda cutar ke yaduwa.[4,5]A halin yanzu, manyan hanyoyin gano ƙwayoyin cuta na numfashi a dakin gwaje-gwaje sun haɗa da: ware ƙwayoyin cuta, gano antigen da gano nucleic acid. Wannan kayan aikin yana gano kuma yana gano takamaiman ƙwayoyin cuta na nucleic acid a cikin mutanen da ke da alamun kamuwa da cutar numfashi, tare da sauran sakamakon asibiti da na dakin gwaje-gwaje don taimakawa wajen gano kamuwa da cutar ƙwayoyin cuta ta numfashi.
Sigogi na Fasaha
| Ajiya | 2-8℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | Swab na hanci; Swab na hanci |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | Kwafi 200/mL |
| Takamaiman Bayani | Reactivity na Cross: Babu wani reactivity tsakanin kit ɗin da ƙwayar cutar Boca, Cytomegalovirus, ƙwayar cutar Epstein-Barr, ƙwayar cutar Herpes simplex, ƙwayar cutar varicella zoster, ƙwayar cutar Mumps, Enterovirus, ƙwayar cutar kyanda, ƙwayar cutar coronavirus ta ɗan adam, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tarin fuka, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivary, Moraxella catarrh, Lactobacillus, Corynebacterium, DNA na halittar ɗan adam. Gwajin tsangwama: Zaɓi mucin (60mg/mL), jinin ɗan adam (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), sodium chloride tare da 5% mai kiyayewa (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), fluniacetone (20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L), The Sakamakon ya nuna cewa abubuwan da ke shiga tsakani a cikin cibiyar da ke sama |
| Kayan Aiki Masu Amfani | An yi amfani da shi don gano nau'in I: Tsarin PCR na Applied Biosystems 7500 Real-Time, QuantStudio®Tsarin PCR guda 5 na Real-Time, Tsarin SLAN-96P na Real-Time (Hongshi Medical Technology Co., Ltd.), Tsarin Gano PCR na Real-Time LineGene 9600 Plus (FQD-96A, fasahar Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Tsarin PCR na Real-Time BioRad CFX96, Tsarin PCR na Real-Time BioRad CFX Opus 96. An yi amfani da shi ga na'urar gano nau'in II: EudemonTM AIO800 (HWTS-EQ007) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Gudun Aiki
Kit ɗin DNA/RNA na Macro & Micro-Test Viral (HWTS-3017) (wanda za a iya amfani da shi tare da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), da Kit ɗin DNA/RNA na Macro & Micro-Test Viral (HWTS-3017-8) (wanda za a iya amfani da shi tare da Eudemon)TM AIO800 (HWTS-EQ007)) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ƙarar samfurin da aka fitar shine 200μL kuma ƙarar fitarwa da aka ba da shawarar shine 150μL.
