Entevirus Universal Universal
Sunan Samfuta
HWTS-EV0011- ADDOVIRIRIRAN GANIN KANO (CLINELESSce PCR)
TopideMology
Cutar da ta hannu-ƙafa tana cutar da cuta ta hanyar onoviruse ta haifar (EV). A halin yanzu, nau'ikan 'yan makamashi 108 na shigar da ƙafa an samo su, waɗanda aka kasu kashi huɗu: A, B, C da D. A cikinsu, Coxa16 sune manyan cututtukan ruwa. Cutar da galibi tana faruwa a cikin yara 'yan kasa da shekaru 5, kuma suna iya haifar da hakki a hannaye, ƙafafunsa, bakin da sauran. Kadan yara za su bunkasa rikice-rikice kamar myocarditis kamar myocarditis, hinga edema, da kuma rikice-rikice na meningoesphales.
Hanya
| Famfo | EV RNA |
| Rox | Kula da ciki |
Sigogi na fasaha
| Ajiya | ≤-18 ℃ |
| Shelf-rayuwa | Watanni 12 |
| Nau'in samfur | Orofharryal Swab,Herpes ruwa |
| Ct | ≤38 |
| CV | ≤5.0% |
| Lod | 500Copies / ml |
| Kayan aiki | Amfani da biosystems 7500/7500 Mai sauri Tsarin PCR na Real-Sysion tsari, Quicstatawa®5 Tsarin PCR na ainihi Slan-96p Real-Lokaci na Real-Lokaci Laima®Tsarin PCR na Real-Lokaci Linegene 9600 da tsarin ganowa na yau da kullun PCR Ma-6000 na lokaci-lokaci na zamani Tsarin cfx96 na lokaci-lokaci na lokaci-lokaci Biorad cfx opus 96 real-lokaci-lokaci tsarin PCR |
Aiki kwarara
Zabi 1.
Ba da shawarar ingirƙirar Ka'idodi: Macro & Micro Kit Jong DNA / RNTE-3017-50 HWTS-3006B, HWTS-30066C), yakamata a fitar da shi tsananin bisa ga umarnin. Yawan samfurin shine 200 μL, shawarar ƙira ƙira shine 80μL.
Zabin 2.
Kit ɗin hakar da aka kawo: Macro & Micro-gwajin saki saki na sake (hwts-3005-8), yakamata a fitar da shi sosai bisa ga umarnin.
Zabi na3.
Kit ɗin hakar da aka kawo: Quaam Victiam Victiam Kudi Kit (52904) ko hakar nucleic acid ko kayan aikin nucleic ko kayan aikin tsarkakewa (YDP3115-R), ya kamata a fitar da shi cikin ƙarfi tare da umarnin. Yawan samfurin shine 140 μL, shawarar ƙira ƙira shine 60μ.
