Enterovirus Universal
Sunan samfurin
Kayan Gano Acid na Nucleic na Duniya na Enterovirus (Fluorescence PCR)
Ilimin Cututtuka
Cutar hannu da ƙafa da baki cuta ce mai yaɗuwa wadda ƙwayoyin cuta masu yaɗuwa ke haifarwa (EV). A halin yanzu, an gano nau'ikan ƙwayoyin cuta iri 108, waɗanda aka raba zuwa ƙungiyoyi huɗu: A, B, C da D. Daga cikinsu, ƙwayoyin cuta masu yaɗuwa EV71 da CoxA16 sune manyan ƙwayoyin cuta. Cutar galibi tana faruwa ne a cikin yara 'yan ƙasa da shekara 5, kuma tana iya haifar da herpes a hannuwa, ƙafafu, baki da sauran sassa. Ƙananan yara za su kamu da matsaloli kamar myocarditis, kumburin huhu, da kuma meningoencephalitis.
Tashar
| FAM | EV RNA |
| ROX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | Tafin hanci,Ruwan herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Kwafi 500/mL |
| Kayan Aiki Masu Amfani | Tsarin Kwamfuta Mai Sauri na 7500/7500, QuantStudio®Tsarin PCR guda 5 na Ainihin Lokaci Tsarin PCR na SLAN-96P na Gaskiya LightCycler®Tsarin PCR na 480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokaci Mai Keke Mai Zafi na MA-6000 na Ainihin Lokaci Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Zaɓi na 1.
Kayan da aka ba da shawarar cirewa: Kayan aikin DNA/RNA na Macro & Micro-Gwaji na Janar (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) da kuma Macro & Micro-Gwaji na atomatik mai cirewa Nucleic Acid (HWTS-3006B, HWTS-3006C), ya kamata a cire shi daidai bisa ga umarnin. Girman samfurin shine 200 μL, girman fitarwa da aka ba da shawarar shine 80µL.
Zaɓi na 2.
Kayan aikin cirewa da aka ba da shawarar: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), ya kamata a cire shi daidai bisa ga umarnin.
Zaɓi na 3.
Kayan aikin cirewa da aka ba da shawarar: Kit ɗin cirewa na QIAamp Viral RNA Mini Kit (52904) ko Kit ɗin cirewa ko tsarkakewa na Nucleic Acid (YDP315-R), ya kamata a cire shi bisa ga umarnin. Girman samfurin shine 140 μL, girman fitarwa da aka ba da shawarar shine 60µL.
