Mycobacterium Tuberculosis INH Resistance
Sunan samfur
HWTS-RT002A-Mycobacterium tarin fuka Isoniazid Resistance Kit (Fluorescence PCR)
Takaddun shaida
Myanmar FDA
Epidemiology
Isoniazid, wani mahimmin maganin cutar tarin fuka da aka gabatar a cikin 1952, yana ɗaya daga cikin magunguna mafi inganci don haɗakar maganin tarin fuka da kuma magani guda ɗaya don cutar ta latent.
KatG shine babban kwayar halittar catalase-peroxidase da maye gurbi na katG na iya haɓaka haɗin bangon tantanin halitta na mycolic acid, yana sa ƙwayoyin cuta su jure wa isoniazid.Maganar KatG tana da alaƙa mara kyau tare da canje-canje a cikin INH-MIC, da raguwar ninki 2 a sakamakon katG a cikin ƙaramin ƙarar ninki 2 a cikin MIC.Wani dalili na juriya na isoniazid a cikin tarin fuka na mycobacterium yana faruwa lokacin shigar da tushe, gogewa ko maye gurbi ya faru a cikin kwayar halittar InhA na tarin fuka na mycobacterium.
Tashoshi
| ROX | inhA (-15C>T) site · |
| CY5 | katG (315G>C). |
| VIC (HEX) | IS6110 |
Ma'aunin Fasaha
| Adana | ≤-18℃ A cikin duhu |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | Sputum |
| CV | ≤5.0% |
| LoD | 1 × 103kwayoyin cuta/ml |
| Musamman | Ba tare da giciye ba tare da maye gurbi na wuraren juriya na magunguna guda huɗu (511, 516, 526 da 531) na rpoB gene a waje da kewayon gano kayan ganowa. Kayayyakin aiki: Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Tsarin PCR na Gaskiya LineGene 9600 Plus Tsarin Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
