Mycoplasma Pneumoniae (MP)
Sunan samfur
HWTS-RT024 Mycoplasma Pneumoniae (MP) Kayan Gano Acid Nucleic (Fluorescence PCR)
Epidemiology
Mycoplasma pneumoniae (MP) wani nau'i ne na ƙananan ƙwayoyin cuta na prokaryotic, wanda ke tsakanin kwayoyin cuta da ƙwayoyin cuta, tare da tsarin tantanin halitta amma babu bangon tantanin halitta.MP ya fi haifar da kamuwa da cututtukan numfashi na mutum, musamman ga yara da matasa.Yana iya haifar da ciwon huhu na mycoplasma na ɗan adam, kamuwa da cutar numfashi na yara da ciwon huhu.Alamomin asibiti daban-daban, yawancinsu sune tari mai tsanani, zazzabi, sanyi, ciwon kai, ciwon makogwaro.Cutar cututtuka na numfashi na sama da ciwon huhu sun fi yawa.Wasu marasa lafiya na iya tasowa daga kamuwa da cutar ta sama zuwa numfashi mai tsanani zuwa ciwon huhu, matsananciyar damuwa na numfashi da mutuwa na iya faruwa.
Tashoshi
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | Sputum, Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 Kwafi/ml |
| Musamman | a) Cross reactivity: babu giciye reactivity da Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus mura, Klebsiella pneumoniae, Klebsiella pneumoniae, Staphylococcus Aurea Pneumoniae, Staphylococcus, Pneumoniae, Staphylococcus Aurea. eruginosa, Acinetobacter baumannii, mura A , Mura B kwayar cuta, Parainfluenza nau'in I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, numfashi syncytial cutar da mutum genomic nucleic acid. B) Ƙarfin tsangwama: babu tsangwama lokacin da aka gwada abubuwan da ke shiga tsakani tare da abubuwan da suka biyo baya: haemoglobin (50mg / L), bilirubin (20mg / dL), mucin (60mg / ml), 10% (v / v) jinin mutum, levofloxacin (10μg / ml), moxifloxacin (0.1g/L), gemifloxacin (80μg/ml), azithromycin (1mg/ml), clarithromycin (125μg/ml), erythromycin (0.5g/L), doxycycline (50mg) /L), minocycline (0.1g/L). |
| Abubuwan da ake Aiwatar da su | Aiwatar da Tsarin Halitta 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR tsarin Tsarin Gano PCR na LineGene 9600 Plus (FQD-96A, fasahar Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
(1) Samfurin Sputum
Shawarar hakar reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za a iya amfani da tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ƙara 200µL na al'ada saline zuwa hazo da aka sarrafa.Ya kamata a yi hakar na gaba bisa ga umarnin don amfani.Girman haɓakar da aka ba da shawarar shine 80µL.An ba da shawarar hakar reagent: Nucleic Acid Extraction or Purification Reagent (YDP315-R).Ya kamata a aiwatar da hakar sosai bisa ga umarnin don amfani.Adadin da aka ba da shawarar shine 60µL.
(2) swab na Oropharyngeal
Shawarar hakar reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za a iya amfani da tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. A hakar ya kamata a yi bisa ga umarnin don amfani.Adadin hakar samfurin da aka ba da shawarar shine 200µL, kuma ƙimar da aka ba da shawarar shine 80µL.Shawarar hakar reagent: QIAamp Viral RNA Mini Kit (52904) ko Nukleic Acid Extraction ko Reagent Reagent (YDP315-R).Ya kamata a aiwatar da hakar sosai bisa ga umarnin don amfani.Adadin da aka ba da shawarar hakar samfurin shine 140µL, kuma ƙimar da aka ba da shawarar shine 60µL.
