Ciwon huhu na Mycoplasma (MP)
Sunan samfurin
Kayan Gano Acid na Nucleic (Fluorescence PCR) na HWTS-RT024
Ilimin Cututtuka
Mycoplasma pneumoniae (MP) wani nau'in ƙananan ƙwayoyin cuta ne na prokaryotic, wanda ke tsakanin ƙwayoyin cuta da ƙwayoyin cuta, tare da tsarin ƙwayoyin halitta amma babu bangon ƙwayoyin halitta. MP galibi yana haifar da kamuwa da cututtukan numfashi na ɗan adam, musamman a cikin yara da matasa. Yana iya haifar da ciwon huhu na mycoplasma na ɗan adam, kamuwa da cututtukan numfashi na yara da kuma ciwon huhu mara tsari. Alamomin asibiti suna da yawa, yawancinsu sune tari mai tsanani, zazzabi, sanyi, ciwon kai, ciwon makogwaro. Cutar huhu ta sama da ciwon huhu na bronchi sune mafi yawan lokuta. Wasu marasa lafiya na iya tasowa daga kamuwa da cutar numfashi ta sama zuwa ciwon huhu mai tsanani, matsananciyar wahalar numfashi da mutuwa na iya faruwa.
Tashar
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | Maƙura, swab na bakin |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Kwafi 200/mL |
| Takamaiman Bayani | a) Haɗakarwa ta hanyar haɗin gwiwa: babu haɗin gwiwa da Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacterium tarin fuka, Legionella pneumophila, Pseudomonas aeruginosa, Acinetobacter baumannii, Influenza A virus, Influenza B virus, Parainfluenza virus type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus da human genomic nucleic acid. b) Ikon hana tsangwama: babu tsangwama lokacin da aka gwada abubuwan da ke hana tsangwama da waɗannan abubuwan: haemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) jinin ɗan adam, levofloxacin (10μg/mL), moxifloxacin (0.1g/L), gemifloxacin (80μg/mL), azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 QuantStudio®Tsarin PCR guda 5 na Ainihin Lokaci Tsarin PCR na SLAN-96P na Gaskiya (Hongshi Medical Technology Co., Ltd.) LightCycler®Tsarin PCR na 480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na Lokaci-lokaci (FQD-96A, fasahar Hangzhou Bioer) MA-6000 Mai Keke Mai Yawan Zafi na Ainihin Lokaci (Suzhou Molarray Co., Ltd.) Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
(1) Samfurin Maniyyi
Maganin cirewa da aka ba da shawarar: Kit ɗin DNA/RNA na Macro & Micro-Test Viral (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za a iya amfani da shi tare da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ƙara 200µL na gishirin yau da kullun a cikin abin da aka sarrafa. Ya kamata a yi cirewa na gaba bisa ga umarnin amfani. Girman cirewa da aka ba da shawarar shine 80µL. Maganin cirewa da aka ba da shawarar: Nucleic Acid Extraction ko Purification Reagent (YDP315-R). Ya kamata a yi cirewa gwargwadon umarnin amfani. Girman cirewa da aka ba da shawarar shine 60µL.
(2) Taɓawar hanci
Maganin cirewa da aka ba da shawarar: Kit ɗin DNA/RNA na Macro & Micro-Test Viral (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za a iya amfani da shi tare da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ya kamata a yi cirewar bisa ga umarnin amfani. Girman cirewar da aka ba da shawarar samfurin shine 200µL, kuma girman fitarwa da aka ba da shawarar shine 80µL. Maganin cirewa da aka ba da shawarar: QIAamp Viral RNA Mini Kit (52904) ko Nucleic Acid Extraction ko Purification Reagent (YDP315-R). Ya kamata a yi cirewar bisa ga umarnin amfani. Girman fitar da samfurin da aka ba da shawarar shine 140µL, kuma girman fitar da samfurin da aka ba da shawarar shine 60µL.
