Binciken Farko na Binciko Abubuwan da ke Cikin Halittar Brucella abortus a Lardin Zhejiang - Ƙungiyar Bioinformatics daga Macro & Micro-Test Ta Ba da Gudummawa ga Bugawa Tare

—— Nazarin Haɗin gwiwa da Zhejiang CDC, Macro & Micro-Test, da China CDC suka buga a cikin Frontiers in Cellular and Infection Microbiology

Bayanin Nazarin

A watan Mayu na 2026, Frontiers in Cellular and Infection Microbiology (JCR Q1, IF ≈ 4.6) sun buga wata takarda da Cibiyar Kula da Cututtuka da Rigakafi ta lardin Zhejiang (Zhejiang CDC) ta jagoranta, tare da ƙungiyar bioinformatics daga Beijing Macro & Micro-Test Bio-Tech Co., Ltd. da kuma Cibiyar Kula da Cututtuka da Rigakafi ta Ƙasa (China CDC) a matsayin waɗanda suka yi haɗin gwiwa. An yi wa binciken taken:
"Binciken ganowa da kuma nazarin halittu na nau'ikan Brucella abortus guda bakwai a Zhejiang, China."

Wannan binciken ya wakilci nazarin gano kwayoyin halitta na farko mai tsari, wanda aka yi bisa ga kwayoyin halitta na Brucella abortus (B. abortus) a lardin Zhejiang, China. Tawagar ta yi nazarin wasu kwayoyin halitta guda bakwai da aka tattara daga 2015 zuwa 2025 (nau'ikan halittu huɗu na asali daga ɗan adam da kuma nau'ikan dabbobi uku na asali daga Jinhua, Quzhou, da Ningbo). Binciken ya ba da shaidar kwayoyin halitta don asali da hanyoyin yaɗuwa na wannan "nau'in halittu masu rinjaye na arewa" a cikin wani yanki na annobar kudanci na gabashin China.

Bayani da Muhimmanci

Brucellosis cuta ce ta dabbobi da ƙwayoyin cuta na dangin Brucella ke haifarwa. Brucella abortus galibi tana kamuwa da shanu amma kuma tana iya haifar da cututtuka ga mutane. A China, brucellosis yana nuna bambancin yanki: mafi yawan faruwar cutar tana faruwa ne a lardunan arewa (misali, Inner Mongolia, Shanxi, Heilongjiang). Sabanin haka, lardunan kudu, ciki har da Zhejiang, a tarihi Brucella melitensis ne suka mamaye su, tare da ƙarancin rahotannin kamuwa da cutar B. abortus. Wannan bambancin yanki ya sa halayyar kwayoyin halitta da kuma gano tushen B. abortus a Zhejiang muhimmin fifiko ne ga lafiyar jama'a.

Hanyoyi da Mahimman Bincike

Ƙungiyar binciken ta ɗauki wata dabara mai matakai da yawa wadda ta haɗa da ilmin kwayoyin halitta da kuma ilimin halittu:

1.Gano ƙwayoyin cuta da kuma rubutaccen asali

Kwayar halittar BCSP-31 PCR da AMOS-PCR sun tabbatar da cewa dukkan kwayoyin halittar guda bakwai na B. abortus ne.

Tsarin rubutu na Multilocus sequence type (MLST) wanda aka yi bisa ga kwayoyin halittar kula da gidaje guda tara ya nuna cewa dukkan abubuwan da aka ware sun kasance na nau'in jerin ST2, wanda ke nuna babban daidaiton kwayoyin halitta tsakanin nau'in B. abortus da ke yawo a Zhejiang.

2.Siffar kwayoyin halitta gaba ɗaya

An gudanar da jerin kwayoyin halitta gaba ɗaya a kan dandamalin Illumina NovaSeq. Binciken matsakaicin asalin nucleotide (ANI) ya nuna cewa isolates na Zhejiang sun yi kama da nau'in B. abortus 544 har zuwa kashi 99.99%.

Binciken kwayoyin halittar da aka gano ya nuna cewa akwai mutane da aka kiyaye sosai: an gano kwayoyin halittar asali guda 3,084, tare da kwayoyin halittar harsashi guda 10 kacal, kuma ba a gano kwayoyin halittar da ke da laushi ko kuma gajimare ba.

3.Bayanan kwayoyin halitta masu juriya ga ƙwayoyin cuta da ƙwayoyin cuta

An yi hasashen jimillar abubuwa 68 da suka shafi kamuwa da cuta, wadanda suka shafi hanyoyin gargajiya kamar su LPS biosynthesis, tsarin fitar da T4SS, da kuma tsarin kula da sassan BvrR-BvrS guda biyu. Abin lura shi ne, duk wadanda aka ware ba su da kwayoyin halittar adhesin bmaA da btaF. Binciken kwayar halittar juriya ya gano kwayar halittar mprF ne kawai a cikin bayanan CARD, ba tare da an gano wasu abubuwan da ke tantance juriya ba.

4. Sake gina kwayoyin halitta da kuma bin diddigin yaduwar cututtuka

Binciken ƙwayoyin halittar Zhejiang guda ɗaya (cgSNP) ya sanya ƙwayoyin Zhejiang a wani matsayi na musamman a cikin bishiyar halittar duniya. Sakamakon ya nuna cewa nau'ikan Zhejiang sun samar da ƙungiyar monophyletic tare da nau'ikan daga Rasha, Mongolia, da wasu lardunan arewacin China (Ningxia, Heilongjiang, Inner Mongolia, Hebei, Gansu, Beijing). Wannan rukunin ya ƙara raba zuwa ƙananan rukuni uku daban-daban (Clade 1-3), yana nuna abubuwan da suka faru na gabatarwa masu zaman kansu da yawa.

Kammalawa da Ma'anoni

Wannan binciken ya samar da babban cikakken bayani game da kwayoyin halittar B. abortus a lardin Zhejiang kuma ya samar da wasu muhimman bayanai:

1. Clear asalin kwayoyin halitta– Nau'in B. abortus da ke yawo a Zhejiang na cikin ST2, an kiyaye su sosai a fannin kwayoyin halitta, kuma suna wakiltar wata irin nau'in brucellosis na shanu.

2. Eviyaduwar cututtuka a yankuna daban-daban– Binciken kwayoyin halitta bai goyi bayan wanzuwar wata zuriya mai zaman kanta a Zhejiang ba. Madadin haka, bayanan sun nuna cewa waɗannan nau'ikan sun samo asali ne daga arewacin China kuma suna iya raba asalin juyin halitta iri ɗaya da nau'ikan daga Rasha da Mongolia. Kasancewar ƙananan rukuni uku yana nufin abubuwan gabatarwa daban-daban.

3. Abubuwan da suka shafi lafiyar jama'a– Binciken ya nuna muhimmancin sa ido kan kwayoyin halitta game da cutar brucellosis ko da a yankunan da ba su da yawa kamar Zhejiang. Duk da cewa adadin wadanda suka kamu da cutar a yanzu bai kai haka ba, kayan aikin da suka fi inganci kamar cgSNP na iya gano tushen barkewar cutar da aka shigo da su ta hanyar amfani da fasahar zamani da kuma samar da shaidar kimiyya don katse hanyoyin watsawa da ke da alaƙa da jigilar dabbobi tsakanin larduna.

Wannan aikin ba wai kawai ya cike gibin bincike a lardin Zhejiang ba ne, har ma ya samar da sabbin bayanai na asali don sa ido kan cututtuka da kuma kimanta haɗarin cutar brucellosis a yankin kogin Yangtze Delta.

Bayanin Takarda:
Yang, Y., Shi, X., Chen, J., Wang, L., Wu, Z., Yao, W., … & Wu, B. (2026). Binciken ganowa da kuma nazarin halittar nau'ikan Brucella abortus guda bakwai a Zhejiang, China. Frontiers in Cellular and Infection Microbiology, 16, 1758965.