Clostridium difficile toxin A/B gene (C.diff)
Sunan samfur
HWTS-OT031A Kayan Gano Acid Nucleic don Clostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Takaddun shaida
CE
Epidemiology
Clostridium difficile (CD), gram-tabbatacce anaerobic sporogenic Clostridium difficile, yana daya daga cikin manyan cututtukan da ke haifar da cututtuka na hanji na nosocomial. A asibiti, kusan 15% ~ 25% na gudawa mai alaƙa da ƙwayoyin cuta, 50% ~ 75% na colitis masu alaƙa da ƙwayoyin cuta da 95% ~ 100% na pseudomembranous enteritis suna haifar da kamuwa da cuta ta Clostridium difficile (CDI). Clostridium difficile cuta ce ta yanayi, gami da nau'ikan guba da nau'ikan da ba su da guba.
Tashoshi
| FAM | tcdAkwayoyin halitta |
| ROX | tcdBkwayoyin halitta |
| VIC/HEX | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | stool |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/ml |
| Musamman | yi amfani da wannan kit ɗin don gano wasu ƙwayoyin cuta na hanji irin su Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, mura A virus, murar mutum sakamakon cutar gero B. |
| Abubuwan da ake Aiwatar da su | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR tsarin Tsarin Gano PCR na LineGene 9600 PlusFQD-96A,HangzhouFasahar Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Zabin 1.
Ƙara 180μL na lysozyme buffer zuwa hazo (diluted lysozyme zuwa 20mg/mL tare da lysozyme diluent), pipette don haɗuwa da kyau, da kuma aiwatarwa a 37 ° C fiye da minti 30. Ɗauki 1.5mL na RNase / DNase-free tube centrifuge tube, da kuma ƙara.180μL na ingantaccen iko da iko mara kyau a cikin jerin. Ƙara10μL na kulawar ciki zuwa samfurin da za a gwada, sarrafawa mai kyau, da iko mara kyau a jere, kuma amfani da Nucleic Acid Extraction or Purification Reagent (YDP302) ta Tiangen Biotech (Beijing) Co., Ltd. don samfurin DNA na gaba, kuma don Allah a bi umarnin don amfani da takamaiman matakai. Yi amfani da DNase/RNase H2O don elution, kuma ƙimar da aka ba da shawarar shine 100μL.
Zabin 2.
Ɗauki 1.5mL na RNase/DNase-free centrifuge tube, kuma ƙara 200μL na ingantaccen iko da mummunan iko a jere. Ƙara10μL na kulawar ciki zuwa samfurin da za a gwada, sarrafawa mai kyau, da sarrafawa mara kyau a cikin jerin, kuma amfani da Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) da Macro & Micro-West Atomatik Nucleic Acid Extractor (30H). Ya kamata a aiwatar da hakar a cikin takamaiman umarnin don amfani, kuma ƙarar da aka ba da shawarar shine 80μL.
