Cobasrium Difultium Toxin A / B Gene (C.DIfl)
Sunan Samfuta
HWTs-ot031a nucleic acid
Takardar shaida
CE
TopideMology
Cobosrium Diffeus (CD), wani masar-tabbatacce Anerobic spitrienifile Diferdium Divistium, yana daya daga cikin manyan cututtukan da ke haifar da cututtukan hanji. Aure, kimanin 15% ~ 25% na maganin kare kai mai dangantaka da kashi 50% ~ 100% na maganin cututtukan Colostium (CDI). Cobosrium Diasuwar pathogen ne, gami da juzu'i mai guba da kuma irin gungumen mara guba.
Hanya
| Famfo | tcdagaren |
| Rox | tcdbgaren |
| Vic / Hex | Kula da ciki |
Sigogi na fasaha
| Ajiya | ≤-18 ℃ |
| Shelf-rayuwa | Watanni 12 |
| Nau'in samfur | matattara |
| Tt | ≤38 |
| CV | ≤5.0% |
| Lod | 200CFU / ml |
| BAYANIN | use this kit to detect other intestinal pathogens such as Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza A virus, influenza B virus and human genomic DNA, da sakamakon duk mara kyau ne. |
| Kayan aiki | Amfani da biosystems 7500 Tsarin aiki na lokaci-lokaci Amfani da biosystems 7500 mai sauri na lokaci-lokaci tsarin tsari Quicstatawa®5 Tsarin PCR na ainihi Slan-96p Real-Real-Lokaci na Real-Lokaci na Real-Lokaci (Kiwon Kuriyar Lantarki na Hongshi Co., Ltd.) Laima®Tsarin PCR na Real-Lokaci Layin 9600 da wani tsarin ganowa na yau da kullun PCR (FQD-96A,HangzhouFasaha ta Bioer) Ma-6000 Real-Time Real-Time Streral Cycler (Suzhou Moharray Co., Ltd.) Tsarin cfx96 na lokaci-lokaci na lokaci-lokaci Biorad cfx opus 96 real-lokaci-lokaci tsarin PCR |
Aiki kwarara
Zabi 1.
Add 180μl na Lysozyme Buffer zuwa ga hazo (tsarma da LySozyme to 20mg / ml tare da bututu mai shekaru 30 / dnase - centrifuge bututu, kuma kara180μl na ingantaccen iko da kuma mummunan iko a jerin. Haɗa10Mulkin da ke cikin gida zuwa samfurin da za a gwada, ikon sarrafawa, da kuma rashin kulawa a cikin jerin abubuwa (Yetp30) ta hanyar hakar DNA, Ltd. don hakar DNA DNA, kuma Da fatan za a bi umarnin don amfani don takamaiman matakai. Yi amfani da dnase / rnase kyauta h2O don ƙaho, da shawarar ƙira ƙira shine 100μl.
Zabin 2.
Takeauki 1.5ml na rnase / DNase-Free Centrifuge butbe, kuma ƙara 200 na ingantaccen iko da kuma mummunan iko a cikin jerin. Haɗa10μL of internal control to the sample to be tested, positive control, and negative control in sequence, and use the Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004- 96) da kuma Macro & micro-gwajin atomatik atomatik (hwts-3006). Ya kamata a aiwatar da hakar a cikin tsayayyen koyarwa don amfani, da shawarar ƙira ƙira shine 80μL.
