Kwayar halittar Clostridium mai wahala ta A/B (C. bambanci)
Sunan samfurin
Kayan Gano Acid na HWTS-OT031A don kwayar halittar Clostridium mai wahala ta gubar A/B (C.diff) (Fluorescence PCR)
Takardar Shaidar
CE
Ilimin Cututtuka
Clostridium difficile (CD), wani nau'in kwayar cuta mai kama da anaerobic sporogenic mai kama da gram-positive Clostridium difficile, yana ɗaya daga cikin manyan ƙwayoyin cuta da ke haifar da cututtukan hanji na nosocomial. A asibiti, kusan kashi 15% ~ 25% na gudawa mai alaƙa da ƙwayoyin cuta, kashi 50% ~ 75% na colitis mai alaƙa da ƙwayoyin cuta da kuma kashi 95% ~ 100% na pseudomembranous enteritis suna faruwa ne ta hanyar kamuwa da cutar Clostridium difficile (CDI). Clostridium difficile cuta ce mai kama da cuta, wacce ta haɗa da nau'ikan guba da nau'ikan da ba sa haifar da guba.
Tashar
| FAM | tcdAkwayar halitta |
| ROX | tcdBkwayar halitta |
| VIC/HEX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | kujera |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Takamaiman Bayani | Yi amfani da wannan kayan aiki don gano wasu cututtukan hanji kamar Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza A virus, influenza B virus da human genomic DNA, sakamakon duk ba su da kyau. |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 QuantStudio®Tsarin PCR guda 5 na Ainihin Lokaci Tsarin PCR na SLAN-96P na Gaskiya (Hongshi Medical Technology Co., Ltd.) LightCycler®Tsarin PCR na 480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokaci (FQD-96A，HangzhouFasahar Bioer) MA-6000 Mai Keke Mai Yawan Zafi na Ainihin Lokaci (Suzhou Molarray Co., Ltd.) Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Zaɓi na 1.
Sai a zuba 180μL na lysozyme buffer a cikin abin da ya fashe (a narkar da lysozyme zuwa 20mg/mL tare da lysozyme diluent), pipette don a gauraya sosai, sannan a sarrafa a zafin jiki na 37°C na tsawon fiye da minti 30. A ɗauki 1.5mL na bututun centrifuge mara RNase/DNase, sannan a ƙara180μL na iko mai kyau da iko mara kyau a jere. Ƙara10μL na ikon sarrafawa na ciki zuwa samfurin da za a gwada, ikon sarrafawa mai kyau, da kuma ikon sarrafawa mara kyau a jere, sannan a yi amfani da Nucleic Acid Extract ko Purification Reagent (YDP302) ta Tiangen Biotech (Beijing) Co., Ltd. don cire samfurin DNA na gaba, kuma da fatan za a bi umarnin da aka bayar don amfani da shi don takamaiman matakai. Yi amfani da DNase/RNase free H2O don cirewa, kuma ƙarar cirewa da aka ba da shawarar shine 100μL.
Zaɓi na 2.
A ɗauki bututun centrifuge mara RNase/DNase 1.5mL, sannan a ƙara 200μL na sarrafawa mai kyau da kuma sarrafawa mara kyau a jere.10μL na ikon sarrafawa na ciki zuwa samfurin da za a gwada, ikon sarrafawa mai kyau, da ikon sarrafawa mara kyau a jere, sannan a yi amfani da Kit ɗin Macro & Micro-Test Viral DNA/RNA (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ya kamata a gudanar da cirewar bisa ga umarnin amfani, kuma girman fitarwa da aka ba da shawarar shine 80μL.
