Cutar Ebola ta Zaire
Sunan samfur
HWTS-FE008 Zaire Cutar Cutar Cutar Kwayar Cutar Nukiliya Acid (Fluorescence PCR)
Epidemiology
Kwayar cutar Ebola ta Filoviridae ce, wacce kwayar cutar RNA ce mara kyau wacce ba ta rabu da ita ba.Kwayoyin cuta dogayen filaments ne masu matsakaicin tsayin virion na 1000nm kuma diamita na kusan 100nm.Kwayar kwayar cutar Ebola wani nau'in RNA ne mara rabo maras kyau wanda girmansa ya kai 18.9kb, yana sanya sunadaran tsarin tsari guda 7 da furotin da ba na tsari ba.Ana iya raba cutar Ebola zuwa nau'ikan kamar su Zaire, Sudan, Bundibugyo, Dajin Tai da Reston.Daga cikin su, nau'in Zaire da nau'in Sudan an ruwaito sun yi sanadiyar mutuwar mutane da dama sakamakon kamuwa da cuta.EHF (Ebola Hemorrhagic Fever) cuta ce mai saurin kamuwa da cuta mai saurin kamuwa da cutar Ebola.Mutane sun fi kamuwa da cutar ta hanyar haɗuwa da ruwan jiki, ɓoyewa da fitar da marasa lafiya ko dabbobin da suka kamu da cutar, kuma alamun asibiti sun fi kamuwa da zazzabi, zubar jini da lalacewar gabobin jiki da yawa.EHF yana da babban adadin mace-mace na 50% -90%.
Tashoshi
| FAM | MP nucleic acid |
| ROX | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 9 |
| Nau'in Samfura | Fresh serum, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kwafi/μL |
| Musamman | Yi amfani da kits don gwada nassoshi mara kyau na kamfani, sakamakon ya cika buƙatun. |
| Abubuwan da ake Aiwatar da su | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Tsarin PCR na Gaskiya LineGene 9600 Plus Tsarin Gano PCR na Gaskiya (FQD-96A, fasahar Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Tsarin PCR na Gaskiya, da BioRad CFX Opus 96 Tsarin PCR na Gaskiya. |
Gudun Aiki
Zabin 1.
Shawarar hakar reagent: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction ko Purification Reagent (YDP315-R) ta Tiangen Biotech (Beijing) Co., Ltd.Ya kamata a fitar da shi daidai da umarnin, kuma ƙimar da aka ba da shawarar shine 140μL kuma ƙimar da aka ba da shawarar ita ce 60μL.
Zabin 2.
Nasihar hakar reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006).It. ya kamata a ciro bisa ga umarnin.Girman samfurin cirewa shine 200μL, kuma ƙimar da aka ba da shawarar shine 80μL.
Zabin 3.
Abubuwan da aka ba da shawarar hakar reagents: Nucleic Acid Extraction Reagent (1000020261) da Babban Tsarin Samfurin Samfurin Shirye-shiryen Shirye-shiryen (MGISP-960) ta BGI yakamata a ciro bisa ga umarnin.Adadin hakar shine 160μL, kuma ƙimar da aka ba da shawarar shine 60μL.
