Zaire Ebola Cutar Kwayar cuta
Sunan Samfuta
HWTS-Fe008 Zaire Ebola gano kayan aikin acid (mai kyalli PCR)
TopideMology
Ebola Cutar Ebola ta kasance ga filin, wanda ba a bayyana ƙwayar cuta mai ban tsoro mai ban tsoro-tawali'u. Kitunan ƙwayoyin cuta suna da dadewa tare da matsakaicin matsakaicin hutun na 1000nm da diamita na game da 100nm. Kwararrun kwayar cutar ta Ebola wacce ba a bayyana ba ce da ta dace da 18.9kb, sun kare sunadarai 7 da furotin 1 da ba na tsari ba. Cutar Ebola za a iya raba ta iri-iri kamar Zaire, Sudan, Bundibugano, dajin tai da hayaniya. Daga cikin su, za a yi rahoton nau'in Zaire da na Sudan da suka haifar da cewa mutane da yawa daga kamuwa da cuta. EHF (Ebola Hemorrhagal zazzabi) wani m batorrafad kamuwa da kamuwa da kamuwa da cutar Ebola. 'Yan Adam galibi suna kamuwa da ruwa da ruwa, asarar jiki da kuma rashin abinci na marasa lafiya ko bayyanar cututtuka da lalacewa ta zira da yawa. Ehf yana da babban mace-mace na 50% -90%.
Hanya
| Famfo | MP nucleic acid |
| Rox | Kula da ciki |
Sigogi na fasaha
| Ajiya | ≤-18 ℃ |
| Shelf-rayuwa | Watanni 9 |
| Nau'in samfur | Fresh Serum, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| Lod | 500 kwafin / μl |
| BAYANIN | Yi amfani da kits don gwada nassoshi marasa kyau, sakamakon yana biyan bukatun. |
| Kayan aiki | Amfani da biosystems 7500 Tsarin aiki na lokaci-lokaci Amfani da biosystems 7500 mai sauri na lokaci-lokaci tsarin tsari QUMSTUDIO®5 Tsarin aiki na lokaci-lokaci Slan-96p Real-Real-Lokaci na Real-Lokaci na Real-Lokaci (Kiwon Kuriyar Lantarki na Hongshi Co., Ltd.) Tsarin PCR na Haske Layin 9600 da wani tsarin ganowa na yau da kullun PCR (FQD-96A, Fasahar Fasaha ta Hangzhou) Ma-6000 Real-Time Real-Time Streral Cycler (Suzhou Moharray Co., Ltd.) Tsarin bincike na yau da kullun na yau da kullun PCR, da biorad cfx opus 96 Real-lokaci-lokaci na lokaci-lokaci tsarin tsari |
Aiki kwarara
Zabi 1.
Mai ba da shawarar hakar da aka ba da shawarar: Quiam Vator Vator Staƙwalwar Kit (52904), hakar nucleic acid ko mai karawa na Tiaangen (YTP) Co., Ltd. Ya kamata a fitar da shi cikin tsananin aiki daidai da umarnin, da kuma shawarar yawan samfurin shine 140μl da shawarar ƙura ƙira ba 60μl.
Zabin 2.
Garfen hakar da aka ba da shawarar sake dawowa: Macro & Micro-TARKO VIRT V micro-> TARIHI NA 3004-34-32, HWS-3004-96) da Macro & Micro-3004-96) da Macro & Micro-3004-96) da kuma macro & micro-3006) .it yakamata a fitar da shi bisa ga umarnin. Yammarar samfurin samfurin shine 200μl, da shawarar ƙura ƙira shine 80μL.
Zabin 3.
Ra'ayin hakar kayan aiki: hakar kayan aiki na nucleic (100002022261) da kuma isasshen samfurin sarrafa samfurin sarrafa kayan aiki (MGISP-960) ta hanyar Bgi ya kamata a fitar da shi bisa ga umarnin. Volar hakar shine 160μL, da shawarar ƙa'idar ƙura ita ce 60μL.
