Cutar Ebola ta Zaire
Sunan samfurin
Kayan Gano Acid na Nucleic (Fluorescence PCR) na Zaire Virus Ebola
Ilimin Cututtuka
Kwayar cutar Ebola ta kasance ta Filoviridae, wacce kwayar cutar RNA ce mai siffar kunkuntar mara tushe wacce ba ta da tushe. Kwayar cuta dogayen filaments ne tare da matsakaicin tsawon virion na 1000nm da diamita na kimanin nm 100. Kwayar cutar Ebola kwayar cuta ce mai siffar RNA wacce ba ta da tushe wacce girmanta ya kai 18.9kb, wacce ke dauke da sunadaran tsari guda 7 da furotin guda 1 wanda ba shi da tsari. Ana iya raba kwayar cutar Ebola zuwa nau'ikan kamar Zaire, Sudan, Bundibugyo, Tai Forest da Reston. Daga cikinsu, an ruwaito cewa nau'in Zaire da nau'in Sudan suna haifar da mutuwar mutane da yawa sakamakon kamuwa da cuta. EHF (Ebola Hemorrhagic Fever) cuta ce mai saurin yaduwa ta hanyar zubar jini da kwayar cutar Ebola ke haifarwa. Mutane galibi suna kamuwa da cutar ne ta hanyar saduwa da ruwan jiki, fitar da ruwa da kuma fitar da ruwa daga jikin marasa lafiya ko dabbobin da suka kamu da cutar, kuma alamun cutar galibi su ne zazzabi mai fitowa, zubar jini da lalacewar gabobin jiki da yawa. EHF tana da yawan mace-mace na kashi 50%-90%.
Tashar
| FAM | MP nucleic acid |
| ROX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 9 |
| Nau'in Samfuri | Fresh serum, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Kwafi 500/μL |
| Takamaiman Bayani | Yi amfani da kayan aikin don gwada nassoshi marasa kyau na kamfanin, sakamakon ya cika buƙatun. |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 Tsarin PCR na Ainihin Lokaci na QuantStudio®5 Tsarin PCR na SLAN-96P na Gaskiya (Hongshi Medical Technology Co., Ltd.) Tsarin PCR na LightCycler®480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus (FQD-96A, fasahar Hangzhou Bioer) MA-6000 Mai Keke Mai Yawan Zafi na Ainihin Lokaci (Suzhou Molarray Co., Ltd.) Tsarin PCR na BioRad CFX96 na Gaskiya, da Tsarin PCR na BioRad CFX Opus 96 na Gaskiya |
Gudun Aiki
Zaɓi na 1.
Shawarar cirewa: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extract or Purification Reagent (YDP315-R) ta Tiangen Biotech (Beijing) Co., Ltd. Ya kamata a cire shi daidai da umarnin, kuma girman cirewa da aka ba da shawarar samfurin shine 140μL kuma girman cirewa da aka ba da shawarar shine 60μL.
Zaɓi na 2.
Shawarar da aka ba da shawarar cirewa: Kit ɗin Macro & Micro-Test Viral DNA/RNA (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ya kamata a cire shi bisa ga umarnin. Girman samfurin cirewa shine 200μL, kuma girman fitarwa da aka ba da shawarar shine 80μL.
Zaɓi na 3.
Shawarar sinadaran cirewa: Ya kamata a cire sinadarin Nucleic Acid Reagent (1000020261) da kuma tsarin shirya samfurin da aka sarrafa ta hanyar amfani da fasahar zamani (MGISP-960) ta BGI bisa ga umarnin. Yawan cirewa shine 160μL, kuma girman fitar da ruwa da aka ba da shawarar shine 60μL.
