Nau'o'i 14 na Cutar Papilloma na ɗan adam mai haɗari (16/18/52 Bugawa)
Sunan samfur
HWTS-CC019A-Nau'o'i 14 na Babban Haɗari na Papillomavirus (16/18/52 Bugawa) Kit ɗin Gano Acid Nucleic (Fluorescence PCR)
Epidemiology
Nazarin ya nuna cewa ciwon daji na HPV da cututtuka masu yawa suna daya daga cikin manyan abubuwan da ke haifar da ciwon daji na mahaifa.A halin yanzu, ana samun ingantattun magunguna waɗanda har yanzu ba su da ciwon sankarar mahaifa da HPV ke haifarwa, don haka gano wuri da rigakafin kamuwa da cutar sankarar mahaifa ta HPV shine mabuɗin hana kansar mahaifa.Yana da matukar mahimmanci don kafa gwaji mai sauƙi, ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ilimin etiology don ganowar asibiti da kuma kula da kansar mahaifa.
Tashoshi
| Tashoshi | Nau'in |
| FAM | HPV 18 |
| VIC/HEX | HPV 16 |
| ROX | HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 |
| CY5 | HPV 52 |
| Kwasar 705/CY5.5 | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | Fitsari, Swab na mahaifa, Swab na Farji |
| Ct | ≤28 |
| LoD | 300 Kwafi/ml |
| Musamman | Babu giciye-reactivity tare da sauran numfashi samfurin kamar mura A, mura B, Legionella pneumophila, Rickettsia Q zazzabi, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneu2 virus, Metapneu. B1/B2, Kwayar cutar syncytial na numfashi A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, da dai sauransu da kuma DNA genomic na mutum. |
| Abubuwan da ake Aiwatar da su | MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Tsarin PCR na Gaskiya da BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
1.Samfurin fitsari
A: Take1.4ml na samfurin fitsari da za'a gwada da centrifuge a 12000rpm na mintuna 5;jefar da supernatant (an bada shawarar kiyaye 10-20μL supernatant daga kasa na centrifuge tube), ƙara 200μL na samfurin reagent reagent, da kuma m hakar ya kamata a gudanar bisa ga umarnin don amfani da Macro & Micro-Test Samfurin Sakin Reagent (HWTS-3005-8).
B: Take1.4ml na samfurin fitsari da za'a gwada kuma a saka a 12,000rpm na mintuna 5;jefar da ma'auni (an bada shawarar kiyaye 10-20μL na supernatant daga kasan bututun centrifuge), kuma ƙara 200μL na saline na al'ada don sake dawowa, a matsayin samfurin da za a gwada.Za a iya gudanar da hakar na gaba tare da Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za'a iya amfani dashi tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. bisa ga umarnin.s don amfani.Adadin da aka ba da shawarar shine 80μL.
C: Take1.4ml na samfurin fitsari da za'a gwada kuma a saka a 12,000rpm na mintuna 5;jefar da ma'auni (an bada shawarar kiyaye 10-20μL na supernatant daga kasan bututun centrifuge), kuma ƙara 200μL na saline na al'ada don sake dawowa, a matsayin samfurin da za a gwada.Za a iya gudanar da hakar na gaba tare daQIAamp DNA Mini Kit(51304) ta QIAGEN ko Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Ya kamata a sarrafa hakar bisa ga umarnin don amfani.Girman samfurin hakar shine 200μL, kuma adadin da aka ba da shawarar shine 80μL.
2. Samfurin swab / farji swab samfurin
A: Ɗauki 1ml na samfurin da za a gwada a cikin 1.5mLof tube centrifuge,kumacentrifuge a 12000rpm na minti 5. Discard the supernatant (an bada shawarar kiyaye 10-20μL na supernatant daga kasan bututun centrifuge), ƙara 100μL na reagent samfurin, sannan cirewa bisa ga umarnin don amfani da Macro & Micro-Test Samfurin Sakin Reagent ( HWTS-3005-8).
B: Ana iya gudanar da hakar tare da Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (wanda za'a iya amfani dashi tare da Macro). & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. bisa ga umarnin don amfani.Girman samfurin da aka fitar shine 200μL, kuma ƙimar da aka ba da shawarar shine 80μL.
C: Ana iya gudanar da hakar tare da QIAamp DNA Mini Kit (51304) ta QIAGEN ko Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).Ya kamata a sarrafa hakar bisa ga umarnin don amfani.Girman samfurin cirewa shine 200 μL, kuma ƙimar da aka ba da shawarar shine80 μl ku.
3.Swab na mahaifa/Farji Swab
Kafin yin samfur, yi amfani da swab ɗin auduga don goge ɓoyayyen ɓoye a hankali daga cervix, sannan a yi amfani da wani swab ɗin auduga da aka shiga tare da maganin adana tantanin halitta ko goga samfurin exfoliated tantanin halitta don manne wa mucosa na mahaifa sannan a juya agogo 3-5 don samun. Kwayoyin exfoliated na mahaifa.A hankali a fitar da swab ɗin auduga ko goga,kumasaka shi a cikin bututun samfurin tare da 1ml na saline na yau da kullun bakararre. Abayan an wanke sosai, sai a busar da swab ɗin auduga ko goga a jikin bangon bututu sannan a jefar da shi, matsa hular bututu, sa'annan a sanya sunan samfurin (ko lamba) sannan a buga akan bututun samfurin.
