Nau'o'in Kwayoyin cuta na Numfashi 4
Sunan samfur
HWTS-RT099-4 nau'ikan ƙwayoyin cuta na Numfashi Kit ɗin Gano Acid Nucleic Acid (Fluorescence PCR)
Epidemiology
Cutar Corona Virus 2019, ana kiranta da "COVID-19", tana nufin ciwon huhu da ke haifarwa.2019-nCoVkamuwa da cuta.2019-nCoVcoronavirus mallakar β genus ne.COVID-19 cuta ce mai saurin kamuwa da cutar numfashi, kuma yawan jama'a gabaɗaya suna da saurin kamuwa da ita.A halin yanzu, tushen kamuwa da cuta shine galibi marasa lafiya da suka kamu da cutar2019-nCoV, kuma masu kamuwa da asymptomatic suma na iya zama tushen kamuwa da cuta.Dangane da binciken cututtukan cututtuka na yanzu, lokacin shiryawa shine kwanaki 1-14, galibi kwanaki 3-7.Zazzabi, bushewar tari da gajiya sune manyan abubuwan da ke bayyana.Wasu marasa lafiya sun sami alamas kamarcunkoson hanci, yawan gudu, ciwon makogwaro, myalgia da gudawa, da dai sauransu.
Tashoshi
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | Farashin B |
| NED | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | -18 ℃ |
| Rayuwar rayuwa | watanni 9 |
| Nau'in Samfura | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300 Kwafi/mlMura A Virus/Cutar cutar mura B/virus syncytial na numfashi: 500Copies/ml |
| Musamman | a) Sakamako-reactivity ya nuna cewa babu wani giciye dauki tsakanin kit da ɗan adam coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza cutar irin 1, 2, 3, Rhinovirus A, B, C, chlamydia pneumoniae, mutum metapneumovirus, enterovirus A, B, C, D, cutar huhu, epstein-barr, cutar kyanda, cutar cytomegalo, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus mura, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tarin fuka, hayaki aspergillus, candida albicans, candidamic acid geroccustiustiki da kuma pneumonia. . b) Ƙarfin tsangwama: zaɓi mucin (60mg / ml), 10% (v / v) na jini da phenylephrine (2mg / mL), oxymetazoline (2mg / ml), sodium chloride (ciki har da masu kiyayewa) (20 mg / ml). beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/ml), fluticasone (2mg/mL). ), histamine hydrochloride (5mg/mL), alpha interferon (800IU/ml), zanamivir (20mg/mL), ribavirin (10mg/ml), oseltamivir (60ng/ml), peramivir (1mg/mL), lopinavir (500mg/ mL, ritonavir (60mg/ml), mupirocin (20mg/ml), azithromycin (1mg/mL), ceftriaxone (40μg/ml), meropenem (200mg/ml), levofloxacin (10μg/ml) da tobramycin (0.6mg/m) mL) don gwajin tsangwama, kuma sakamakon ya nuna cewa abubuwa masu tsangwama tare da abubuwan da aka ambata a sama ba su da wani tsangwama ga sakamakon gwajin ƙwayoyin cuta. |
| Abubuwan da ake Aiwatar da su | Aiwatar da Tsarin Halitta 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya |
Gudun Aiki
Zabin 1.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006) wanda Jiangsu Macro & Micro ya samar. -Test Med-Tech Co., Ltd. Ƙarar samfurin da aka fitar shine 200μL, kuma ƙimar da aka ba da shawarar shine 80μL.
Zabin 2.
QIAamp Viral RNA Mini Kit (52904) wanda QIAGEN ko Nucleic Acid Extraction ko Tsarkake Kit (YDP315-R) wanda Tiangen Biotech (Beijing) Co., Ltd ya samar. Ƙarar samfurin da aka fitar shine 140μL, kuma ƙimar da aka ba da shawarar shine 60μL.
