Adenovirus Universal
Sunan samfurin
Kayan Gano Acid na Nucleic na Duniya na HWTS-RT017A na Adenovirus (Fluorescence PCR)
Ilimin Cututtuka
Cutar adenovirus ta ɗan adam (HAdV) ta samo asali ne daga dangin Mammalian adenovirus, wanda kwayar cutar DNA ce mai nau'i biyu ba tare da wani abu a cikinta ba. Cutar adenovirus da aka gano zuwa yanzu ta haɗa da ƙananan ƙungiyoyi 7 (AG) da nau'ikan 67, waɗanda nau'ikan serotypes 55 ke haifar da cututtuka ga mutane. Daga cikinsu, waɗanda za su iya haifar da cututtukan numfashi galibi rukuni na B (Nau'i na 3, 7, 11, 14, 16, 21, 50, 55), Rukunin C (Nau'i na 1, 2, 5, 6, 57) da Rukunin E (Nau'i na 4), kuma za su iya haifar da kamuwa da gudawa ta hanji rukuni na F (Nau'i na 40 da 41)[1-8]. Nau'i daban-daban suna da alamun asibiti daban-daban, amma galibi kamuwa da cututtukan numfashi. Cututtukan numfashi da cututtukan numfashi na jikin ɗan adam ke haifarwa sun kai kashi 5% ~15% na cututtukan numfashi na duniya, da kuma kashi 5% -7% na cututtukan numfashi na yara na duniya[9]. Cutar Adenovirus ta yaɗu a wurare daban-daban kuma ana iya kamuwa da ita duk shekara, musamman a wuraren da ke cike da cunkoso, waɗanda ke da saurin kamuwa da cutar a cikin gida, galibi a makarantu da sansanonin sojoji.
Tashar
| FAM | adenovirus na duniyasinadarin nucleic acid |
| ROX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | Swab na hanci，Swab na makogwaro |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Kwafi 300/mL |
| Takamaiman Bayani | a) Gwada nassoshi marasa kyau na kamfanin da aka tsara ta hanyar kayan aikin, kuma sakamakon gwajin ya cika sharuɗɗan. b) Yi amfani da wannan kayan aiki don gano kuma babu wani haɗin gwiwa tsakanin wasu ƙwayoyin cuta na numfashi (kamar ƙwayar cuta ta mura A, ƙwayar cuta ta mura B, ƙwayar cuta ta numfashi, ƙwayar cuta ta Parainfluenza, ƙwayar cuta ta Rhinovirus, ƙwayar cuta ta metapneumovirus ta ɗan adam, da sauransu) ko ƙwayoyin cuta (Streptococcus pneumoniae, ƙwayar cuta ta Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, da sauransu). |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 Tsarin PCR na Ainihin Lokaci na QuantStudio®5 Tsarin PCR na SLAN-96P na Gaskiya (Hongshi Medical Technology Co., Ltd.) Tsarin PCR na LightCycler®480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokacis (FQD-96A, HangzhouFasahar Bioer) MA-6000 Mai Keke Mai Yawan Zafi na Ainihin Lokaci (Suzhou Molarray Co., Ltd.) Tsarin PCR na BioRad CFX96 na Gaskiya, Tsarin PCR na BioRad CFX Opus 96 na Gaskiya |
Gudun Aiki
(1) Shawarar cirewa:Maganin Sample Release Release na Macro & Micro (HWTS-3005-8). Ya kamata a yi cirewar bisa ga umarnin. Samfurin da aka cire shine marasa lafiya.'Samfuran swab na nasopharyngeal ko swab na makogwaro da aka tattara a wurin. A zuba samfuran a cikin sinadarin fitar da samfurin ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex don a gauraya sosai, a sanya a zafin ɗaki na minti 5, a cire sannan a juya a gauraya sosai don samun DNA na kowane samfurin.
(2) Shawarar cirewa:Gwajin Macro & Micro Kwayar cuta mai yaɗuwa (Viral) Kayan DNA/RNA(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) da kuma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).Ya kamata a yi aikin bisa ga umarnin da aka bayar. Yawan samfurin da aka fitar shine 200.μL, kumaƙarar fitar da ruwa da aka ba da shawararis80ml.
(3) Maganin cirewa da aka ba da shawarar: Cirewar Acid ta Nucleic ko Maganin tsarkakewa (YDP)315) ta Tiangen Biotech (Beijing) Co.,Ltd., daYa kamata a yi aikin bisa ga umarnin da aka bayar. Yawan samfurin da aka fitar shine 200.μL, kumaƙarar fitar da ruwa da aka ba da shawararis80ml.
