Herpes Simplex Virus Type 2 Nucleic Acid
Sunan samfur
HWTS-UR007A-Herpes Simplex Virus Type 2 Nucleic Acid Detection Kit (Fluorescence PCR)
Amfani da Niyya
Ana amfani da wannan kit ɗin don gano ingancin ƙwayar cutar ta herpes simplex nau'in 2 nucleic acid a cikin swab na urethra na namiji da samfuran swab na mahaifa na mace.
Epidemiology
Herpes Simplex Virus Type 2 (HSV2) kwayar cuta ce ta madauwari da aka haɗa tare da tegument, capsid, core, da ambulaf, kuma ya ƙunshi DNA mai layi mai layi biyu.Kwayar cutar ta Herpes na iya shiga cikin jiki ta hanyar saduwa ta kai tsaye ko saduwa da fata da mucosa, kuma ta kasu kashi na farko da maimaituwa.Cutar sankarau tana faruwa ne ta hanyar HSV2, marasa lafiya maza suna bayyana a matsayin gyambon azzakari, kuma mata mata suna bayyana a matsayin ciwon mahaifa, vulvar, da na farji.Cututtukan farko na kwayar cutar ta al’aura galibi cututtuka ne na koma baya, sai dai wasu ’yan ciwon daji masu dauke da mucosa ko fata, wadanda yawancinsu ba su da alamun bayyanar cututtuka na asibiti.Kamuwa da cuta na al'aura yana da halayen ƙwayoyin cuta na rayuwa da kuma sake dawowa cikin sauƙi, kuma duka marasa lafiya da masu ɗauka sune tushen kamuwa da cutar.A kasar Sin, madaidaicin ƙimar HSV2 yana da kusan 10.80% zuwa 23.56%.Za'a iya raba matakin kamuwa da cutar HSV2 zuwa kamuwa da cuta ta farko da kamuwa da cuta mai maimaitawa, kuma kusan kashi 60% na marasa lafiya na HSV2 sun sake komawa.
Epidemiology
FAM: Herpes Simplex Virus Type 2 (HSV2) ·
VIC(HEX): Gudanar da Ciki
Saitin Haɓaka Yanayin PCR
Mataki | Zagaye | Zazzabi | Lokaci | TattaraFluorescentSignalsko babu |
1 | 1 Zagayowar | 50 ℃ | 5 min | No |
2 | 1 Zagayowar | 95 ℃ | Minti 10 | No |
3 | Zagaye 40 | 95 ℃ | dakika 15 | No |
4 | 58 ℃ | dakika 31 | Ee |
Ma'aunin Fasaha
Adana | |
Ruwa | ≤-18℃ A cikin duhu |
Rayuwar rayuwa | watanni 12 |
Nau'in Samfura | Tashin mahaifa na mace, swab na maza |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 Kwafi/ martani |
Musamman | Babu giciye-reactivity tare da sauran STD pathogens, irin su Treponema pallidum, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium da sauransu. |
Abubuwan da ake Aiwatar da su | Zai iya dacewa da kayan aikin PCR na yau da kullun akan kasuwa. Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Tsarin PCR na Gaskiya LineGene 9600 Plus Tsarin Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya. |