Human Cytomegalovirus (HCMV) Nucleic Acid
Sunan samfur
HWTS-UR008A-Human cytomegalovirus (HCMV) kayan gano nucleic acid (Fluorescence PCR)
Epidemiology
Mutum cytomegalovirus (HCMV) memba ne mai mafi girma a cikin kwayar cutar ta herpes kuma yana iya ɓoye sunadaran sunadaran fiye da 200.HCMV yana da ɗan taƙaitawa a cikin kewayon rundunarsa ga mutane, kuma har yanzu babu samfurin dabba na kamuwa da cuta.HCMV yana da jinkirin sake zagayowar maimaitawa mai tsawo don samar da jiki mai haɗawa cikin intranuclear, kuma yana haifar da samar da jikunan haɗaɗɗun perinuclear da cytoplasmic da kumburin tantanin halitta (giant cell), saboda haka sunan.Dangane da nau'in nau'in kwayar halitta da nau'in halitta, HCMV za a iya raba shi zuwa nau'i-nau'i iri-iri, daga cikinsu akwai wasu bambance-bambancen antigenic, waɗanda, duk da haka, ba su da wani mahimmanci na asibiti.
HCMV kamuwa da cuta cuta ce ta tsarin, wanda a asibiti ya ƙunshi gabobin da yawa, yana da hadaddun alamu daban-daban, galibi shiru ne, kuma yana iya haifar da wasu marasa lafiya don haɓaka cututtukan ƙwayoyin cuta da yawa ciki har da retinitis, hepatitis, ciwon huhu, encephalitis, colitis, monocytosis, da thrombocytopenic. purpura.Cutar HCMV ta zama ruwan dare kuma yana yaduwa a duniya.Yana da yawa a cikin yawan jama'a, tare da adadin abubuwan da suka faru na 45-50% da fiye da 90% a cikin kasashe masu tasowa da masu tasowa, bi da bi.HCMV na iya kwanciya barci a cikin jiki na dogon lokaci.Da zarar garkuwar jiki ta yi rauni, kwayar cutar za a kunna ta don haifar da cututtuka, musamman kamuwa da cutar sankarar bargo da masu dashewa, kuma tana iya haifar da dashen gabobin jiki da kuma jefa rayuwar marasa lafiya cikin mawuyacin hali.Bugu da ƙari ga haifuwa, zubar da ciki da haihuwa da wuri ta hanyar kamuwa da cuta ta ciki, cytomegalovirus kuma na iya haifar da rashin lafiya na haihuwa, don haka kamuwa da cutar HCMV yana iya rinjayar kulawar haihuwa da haihuwa da kuma ingancin yawan jama'a.
Tashoshi
FAM | Farashin HCMV |
VIC(HEX) | Ikon Cikin Gida |
Ma'aunin Fasaha
Adana | Liquid: ≤-18 ℃ A cikin duhu |
Rayuwar rayuwa | watanni 12 |
Nau'in Samfura | Samfurin Magani, Samfurin Plasma |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 Kwafi/ martani |
Musamman | Babu wani abin da ke faruwa tare da cutar hanta B, cutar hepatitis C, kwayar cutar papilloma na mutum, nau'in kwayar cutar ta herpes simplex 1, nau'in kwayar cutar ta 2, samfurori na al'ada na mutum, da dai sauransu. |
Kayayyakin aiki: | Zai iya dacewa da kayan aikin PCR na yau da kullun akan kasuwa. Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya Abubuwan da aka Aiwatar da Tsarin Halittu 7500 Tsarin PCR Mai Saurin Real-Time QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Tsarin PCR na Gaskiya LineGene 9600 Plus Tsarin Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96 (wanda za a iya amfani da tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ya kamata a fitar da hakar bisa ga umarnin.Girman samfurin cirewa shine 200μL, kuma ƙimar da aka ba da shawarar shine 80μL.
Shawarar hakar reagent: QIAamp DNA Mini Kit (51304), Nucleic Acid Extraction ko Purification Reagent (YDP315) ta Tiangen Biotech (Beijing) Co., Ltd.ya kamata a fitar da shi bisa ga umarnin cirewa, kuma ƙimar da aka ba da shawarar shine 200 μL kuma ƙimar da aka ba da shawarar shine 100 μL.