Canjin Halittar Haɗakar TEL-AML1 na ɗan adam
Sunan samfurin
Kayan Gano Canjin Halittar Haɗakar Halittar HWTS-TM016 na Ɗan Adam TEL-AML1 (Fluorescence PCR)
Ilimin Cututtuka
Cutar sankarar lymphoblastic mai tsanani (ALL) ita ce cutar da ta fi kamari a yara. A cikin 'yan shekarun nan, cutar sankarar lymphoblastic mai tsanani (AL) ta canza daga nau'in MIC (morphology, immunology, cytogenetics) zuwa nau'in MICM (ƙarin gwajin ilmin halitta na kwayoyin halitta). A shekarar 1994, an gano cewa haɗakar TEL a lokacin ƙuruciya ta samo asali ne daga canjin chromosomal t(12;21)(p13;q22) a cikin cutar sankarar lymphoblastic mai tsanani ta B-lineage (ALL). Tun lokacin da aka gano kwayar halittar AML1, kwayar halittar haɗakar TEL-AML1 ita ce hanya mafi kyau don tantance hasashen yara masu cutar sankarar lymphoblastic mai tsanani.
Tashar
| FAM | Tsarin halittar haɗin TEL-AML1 |
| ROX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 9 |
| Nau'in Samfuri | samfurin ƙashi |
| Ct | ≤40 |
| CV | <5.0% |
| LoD | Kwafi 1000/mL |
| Takamaiman Bayani | Babu wani haɗin gwiwa tsakanin kayan aiki da sauran kwayoyin halittar haɗuwa kamar kwayoyin halittar haɗuwar BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, da PML-RARa. |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 Tsarin PCR Mai Sauri na Ainihin Lokaci Mai Amfani da Biosystems 7500 Tsarin PCR na Ainihin Lokaci na QuantStudio®5 Tsarin PCR na SLAN-96P na Gaskiya Tsarin PCR na LightCycler®480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokaci Mai Keke Mai Zafi na MA-6000 na Ainihin Lokaci Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Kit ɗin Cire RNA na Jini Mai Tsarki na RNAprep (DP433).
