Kayan Gano Canje-canje na KRAS 8 (Fluorescence PCR)-RUO
Sunan samfurin
Kayan Gano Canje-canje na KRAS 8 (Fluorescence PCR)-RUO
Ilimin Cututtuka
An gano maye gurbi a cikin kwayar halittar KRAS a cikin nau'ikan ciwon daji na ɗan adam da dama, kimanin kashi 17% ~ 25% na yawan maye gurbi a cikin ƙari, kashi 15% ~ 30% na yawan maye gurbi a cikin marasa lafiya da ke fama da ciwon daji na huhu, kashi 20% ~ 50% na yawan maye gurbi a cikin marasa lafiya da ke fama da ciwon daji na hanji. Saboda furotin na P21 da kwayar halittar K-ras ta ƙunsa yana ƙasa da hanyar siginar EGFR, bayan maye gurbi a cikin kwayar halittar K-ras, hanyar siginar da ke ƙasa koyaushe tana aiki kuma magungunan da aka yi niyya a sama ba sa shafar su, wanda ke haifar da ci gaba da yaduwar ƙwayoyin cuta masu cutarwa. Sauye-sauye a cikin kwayar halittar K-ras gabaɗaya yana ba da juriya ga masu hana EGFR tyrosine kinase a cikin marasa lafiya da ke fama da ciwon daji na huhu da kuma juriya ga magungunan anti-EGFR a cikin marasa lafiya da ke fama da ciwon daji na hanji [1, 2, 3]. A shekara ta 2008, Cibiyar Kula da Ciwon Daji ta Kasa (NCCN) ta fitar da wata ka'ida ta aikin asibiti don cutar kansar hanji, wadda ta nuna cewa wuraren maye gurbi da ke haifar da kunna K-ras galibi suna cikin codons 12 da 13 na exon 2, kuma ta ba da shawarar cewa duk marasa lafiya da ke da ciwon daji na hanji mai tsanani za a iya gwada su don maye gurbi na K-ras kafin a yi musu magani[4]. Saboda haka, gano maye gurbi na kwayar halittar K-ras cikin sauri da daidaito yana da matukar muhimmanci a cikin jagorar magunguna na asibiti. Wannan kayan aikin yana amfani da DNA a matsayin samfurin ganowa don samar da kimantawa mai inganci na matsayin maye gurbi, wanda zai iya taimaka wa likitoci wajen tantance cutar kansar hanji, cutar kansar huhu da sauran marasa lafiya da ke fama da ciwon daji waɗanda ke amfana da magunguna da aka yi niyya. Sakamakon gwajin kayan aikin don amfani ne kawai kuma bai kamata a yi amfani da shi azaman tushen magani na musamman ga marasa lafiya ba. Ya kamata likitocin asibiti su yanke hukunci mai zurfi kan sakamakon gwajin bisa ga abubuwan da suka shafi yanayin majiyyaci, alamun magani, amsawar magani da sauran alamun gwajin dakin gwaje-gwaje.
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | sassan cututtukan da aka saka a cikin paraffin na ɗan adam |
| CV | ≤5.0% |
| LoD | a) Buffer na K-ras Reaction Buffer A da Buffer na K-ras Reaction B na iya gano ƙimar maye gurbi na 1% a ƙarƙashin asalin nau'in daji na 3ng/μL; b) Sauyin 1×103Ana iya gano kwafi/mL a cikin yanayin daji na 1×105Kwafi/mL lokacin da ƙimar sauye-sauyen ya kasance 1%; c) Lokacin da aka gwada bayanin kamfanin LoD SW3, babu ƙimar Ct ko ƙimar Ct = 0 na Reaction Buffer A da Reaction Buffer B |
| Kayan Aiki Masu Amfani | Tsarin Biosystems 7500 na Gaskiya, Tsarin Biosystems 7300 na Gaskiya, Tsarin PCR na Gaskiya na QuantStudio®5, Tsarin PCR na Gaskiya na LightCycler® 480, Tsarin PCR na Gaskiya na BioRad CFX96. |
Gudun Aiki
Ana buƙatar mai hana sake amfani da sinadarai amma ba a bayar da shi ba:H2O mara DNase/RNase, ethanol mai hana ruwa shiga. Lokacin gwada samfurin nama mai ɗauke da paraffin, ana ba da shawarar amfani da Kit ɗin Tissue na QIAamp DNA FFPE (56404) na QIAGEN da Kit ɗin Extraction na DNA mai ɗauke da Paraffin (DP330) wanda Tiangen Biotech(Beijing) Co., Ltd. suka ƙera.
Ana buƙatar kayan amfani amma ba a bayar da su ba:Nau'ikan da ba su da DNase/RNase, safar hannu da za a iya yarwa, bututun centrifuge marasa DNase/RNase, tsiri mai bututu 8, centrifuge.
