Kayan Gano Acid na Nucleic da aka Busasshe a Cutar Dengue I/II/II/IV (Fluorescence PCR)-RUO
Sunan samfurin
Kayan Gano Acid na Nucleic da aka Busasshe a Cutar Dengue I/II/II/IV (Fluorescence PCR)-RUO
Ilimin Cututtuka
Zazzabin Dengue (DF), wanda kamuwar denguevirus (DENV) ke haifarwa, yana ɗaya daga cikin cututtukan da suka fi yaɗuwa arbovirus. Maganin ya haɗa da Aedes aegypti da Aedes albopictus. DF galibi ya fi yaɗuwa a yankunan zafi da kuma yankunan da ke ƙarƙashin zafi. DENV na cikin flavivirus a ƙarƙashin flaviviridae, kuma ana iya rarraba shi zuwa nau'ikan serotypes guda 4 bisa ga antigen na saman jiki. Bayyanar cututtukan DENV galibi sun haɗa da ciwon kai, zazzabi, rauni, faɗaɗa lymph node, leukopenia da sauransu, da zubar jini, girgiza, raunin hanta ko ma mutuwa a cikin mawuyacin hali. A cikin 'yan shekarun nan, sauyin yanayi, ƙaura zuwa birane, saurin haɓaka yawon buɗe ido da sauran abubuwa sun samar da yanayi mai sauri da dacewa don yaɗawa da yaɗuwar DF, wanda ke haifar da ci gaba da faɗaɗa yankin annoba na DF. Saboda haka, kafa hanyar ganewar asali mai sauƙi, takamaiman kuma mai sauri ta DENV yana da mahimmanci ga ganewar cutar DF.
Sigogi na Fasaha
| Ajiya | 2-8℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | magani na serum |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Kwafi 500/mL |
| Takamaiman Bayani | Gwaje-gwajen ba su da kyau ga samfuran jini na cutar dengue marasa kyau; sakamakon gwajin tsangwama ya nuna cewa lokacin da yawan bilirubin a cikin jini bai wuce 168.2μmol/mL ba, yawan haemoglobin da hemolysis ke samarwa bai wuce 130g/L ba, yawan lipids a cikin jini bai wuce 65mmol/mL ba, kuma jimlar yawan IgG a cikin jini bai wuce 5mg/mL ba, babu wani tasiri akan gano kwayar cutar dengue; yi amfani da kwayar cutar Hepatitis A, kwayar cutar Hepatitis B, kwayar cutar Hepatitis C, kwayar cutar Herpes, kwayar cutar Eastern equine encephalitis, kwayar cutar Hantaan, kwayar cutar Bunia, kwayar cutar West Nile, kwayar cutar Zika da samfuran kwayoyin halittar mutum don gwajin amsawar giciye, kuma sakamakon ya nuna cewa babu wani martani tsakanin wannan kayan aiki da kwayoyin cuta da ke sama. |
| Kayan Aiki Masu Amfani | Nau'i na I: Tsarin PCR na Zamani na Applied Biosystems 7500, Tsarin PCR na Zamani na Zamani na QuantStudio®5, Tsarin PCR na Zamani na Zamani na SLAN-96P (Hongshi Medical Technology Co., Ltd.), Tsarin Gano PCR na Zamani na LineGene 9600 Plus (FQD-96A, fasahar Hangzhou Bioer), MA-6000 Mai Zane ... Nau'i na II: EudemonTMAIO800 (HWTS-EQ007) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
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Gudun Aiki
Ana buƙatar masu maye amma ba a bayar da su ba:
Nau'i na I: Kit ɗin DNA/RNA na Macro & Micro-Test General (HWTS-3017) (wanda za a iya amfani da shi tare da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022).
Nau'i na II: Kayan gwajin DNA/RNA na Macro & Micro-Gwaji na Janar (HWTS-3023-8), ana iya amfani da shi tare da EudemonTM AIO800 (HWTS-EQ007) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kafin amfani da na'urar gwajin Type II, abokin ciniki yana da na'urar cirewa da kayan aikin ƙara girma, kuma injiniyan zai jagoranci abokin ciniki don gudanar da aikin gwaji lokacin amfani da shi a karon farko.
Ana buƙatar kayan amfani amma ba a bayar da su ba:Nasihohi marasa amfani na DNase/RNase, safar hannu da za a iya zubarwa, bututun EP marasa amfani na DNase/RNase, centrifuge, wurin maganadisu, tsiri na bututu 8 don PCR.
