Kayan Gano Canje-canje na Halittar Dan Adam EGFR 29 (Fluorescence PCR)-RUO

Ciwon daji na huhu ya zama babban abin da ke haifar da mace-macen ciwon daji a duk duniya, wanda hakan ke barazana ga lafiyar ɗan adam. Ciwon daji na huhu wanda ba ƙaramin ƙwayar halitta ba ne ke da alhakin kusan kashi 80%-85% na marasa lafiya da ke fama da ciwon daji na huhu. EGFR a halin yanzu shine mafi mahimmancin abin da ake nufi da maganin ciwon daji na huhu wanda ba ƙaramin ƙwayar halitta ba ne. Phosphorylation na EGFR na iya haɓaka haɓakar ƙwayoyin cuta, bambance-bambance, mamayewa, metastasis, hana apoptosis, da kuma haɓaka angiogenesis na ciwon daji. Masu hana EGFR tyrosine kinase (TKI) na iya toshe hanyar siginar EGFR ta hanyar hana haɓakar EGFR autophosphorylation, ta haka yana hana yaduwa da bambance-bambancen ƙwayoyin cuta, yana haɓaka apoptosis na ƙwayoyin cuta, rage angiogenesis na ciwon daji, da sauransu, don cimma maganin da aka yi niyya ga ciwon daji [1]. Yawancin bincike sun nuna cewa ingancin maganin EGFR-TKI yana da alaƙa da matsayin maye gurbin kwayar halittar EGFR, kuma yana iya hana haɓakar ƙwayoyin cuta tare da maye gurbin kwayar halittar EGFR [2,3]. Kwayar halittar EGFR tana kan gajeren hannun chromosome 7 (7p12), tare da cikakken tsawon 200Kb kuma ta ƙunshi exons 28. Yankin maye gurbi galibi yana cikin exons 18 zuwa 21, maye gurbi na share codons 746 zuwa 753 akan exon 19 yana da kusan kashi 45% kuma maye gurbi na L858R akan exon 21 yana da kusan kashi 40% zuwa 45% [4]. Jagororin NCCN don Ganewa da Maganin Ciwon Huhu Marasa Ƙaramin Kwayar Halitta ya bayyana a sarari cewa ana buƙatar gwajin maye gurbi na kwayar halitta ta EGFR kafin a fara amfani da EGFR-TKI. Ana amfani da wannan kayan gwaji don jagorantar gudanar da magungunan epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), kuma yana ba da tushen magani na musamman ga marasa lafiya da ciwon huhu mara ƙaramin kwayar halitta. Ana amfani da wannan kayan ne kawai don gano maye gurbi gama gari a cikin kwayar halittar EGFR a cikin marasa lafiya da ciwon huhu mara ƙaramin kwayar halitta. Sakamakon gwajin an yi shi ne kawai don amfani da asibiti kuma bai kamata a yi amfani da shi azaman tushen magani na musamman ga marasa lafiya ba. Ya kamata likitocin asibiti su yi la'akari da yanayin majiyyaci, alamun magani, da magani. Ana amfani da martanin da sauran alamun gwajin dakin gwaje-gwaje da sauran abubuwa don yin cikakken hukunci kan sakamakon gwajin.