Mycobacterium Tuberculosis INH Mutation
Sunan samfur
HWTS-RT137 Mycobacterium Tuberculosis INH Kayan Gane Maɓalli (Narkewar Curve)
Epidemiology
Mycobacterium tarin fuka, jim kadan a matsayin Tubercle bacillus (TB), ita ce kwayar cutar da ke haifar da tarin fuka. A halin yanzu, magungunan da ake amfani da su na farko na maganin tarin fuka sun hada da INH, rifampicin da hexambutol, da dai sauransu. Na biyu na maganin tarin fuka sun hada da fluoroquinolones, amikacin da kanamycin, da dai sauransu. Sabbin magungunan da aka bunkasa sune linezolid, bedaquiline da delamani, da dai sauransu, duk da haka, saboda rashin amfani da magungunan maganin tarin fuka da kuma tsarin kwayoyin cutar my tarin fuka. Mycobacterium tarin fuka yana haɓaka juriya na ƙwayoyi ga magungunan tarin fuka, wanda ke kawo ƙalubale mai tsanani ga rigakafi da maganin tarin fuka.
Tashoshi
| FAM | MP nucleic acid |
| ROX | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | sputum |
| CV | ≤5% |
| LoD | Iyakar ganowa na nau'in ƙwayoyin cuta na INH na daji shine 2x103 ƙwayoyin cuta/mL, kuma iyakar ganowa ga ƙwayoyin cuta na mutant shine 2x103 ƙwayoyin cuta/mL. |
| Musamman | a. Babu wani ra'ayi tsakanin kwayoyin halittar ɗan adam, sauran ƙwayoyin cuta na mycobacteria marasa tuberculous da cututtukan huhu da wannan kayan ya gano.b. An gano wuraren maye gurbi na sauran kwayoyin halittar da ke jure magunguna a cikin nau'in cutar tarin fuka na Mycobacterium, kamar juriya da ke kayyade yanki na kwayar rifampicin rpoB, kuma sakamakon gwajin ya nuna babu juriya ga INH, wanda ke nuni da cewa babu giciye. |
| Abubuwan da ake Aiwatar da su | SLAN-96P Tsarin PCR na GaskiyaBioRad CFX96 Tsarin PCR na GaskiyaLightCycler480®Tsarin PCR na Gaskiya |
Gudun Aiki
Idan amfani da Macro & Micro-Test Janar DNA / RNA Kit (HWTS-3019) (wanda za'a iya amfani dashi tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. za a gwada samfurin sarrafawa da ƙari na 20000L don sarrafa samfurin, ƙara samfurin 2000000L. a jere, kuma ƙara 10μL na kulawar ciki daban a cikin iko mara kyau, samfurin sputum da aka sarrafa don gwadawa, kuma matakan da suka biyo baya yakamata a aiwatar dasu daidai da umarnin cirewa. Girman samfurin da aka fitar shine 200μL, kuma ƙimar da aka ba da shawarar shine 100μL.
