Canjin Tarin Fuka na Mycobacterium INH
Sunan samfurin
Kayan Gano Canjin Halittar HWTS-RT137 na Mycobacterium Tuberculosis INH (Narkewar Lanƙwasa)
Ilimin Cututtuka
Tarin fuka na Mycobacterium, wanda aka fi sani da Tubercle Bacillus (TB), shine kwayar cutar da ke haifar da tarin fuka. A halin yanzu, magungunan rigakafin tarin fuka na farko da ake amfani da su sun hada da INH, rifampicin da hexambutol, da sauransu. Magungunan rigakafin tarin fuka na biyu sun hada da fluoroquinolones, amikacin da kanamycin, da sauransu. Sabbin magungunan da aka haɓaka sune linezolid, bedaquiline da delamani, da sauransu. Duk da haka, saboda rashin amfani da magungunan rigakafin tarin fuka daidai ba da kuma halayen tsarin bangon tantanin halitta na tarin fuka na mycobacterium, tarin fuka na mycobacterium yana haɓaka juriya ga magunguna ga magungunan rigakafin tarin fuka, wanda ke kawo ƙalubale masu tsanani ga rigakafi da maganin tarin fuka.
Tashar
| FAM | MP nucleic acid |
| ROX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | man shafawa |
| CV | ≤5% |
| LoD | Iyakar gano ƙwayoyin cuta na INH na daji shine ƙwayoyin cuta 2x103/mL, kuma iyaka ga gano ƙwayoyin cuta masu canzawa shine ƙwayoyin cuta 2x103/mL. |
| Takamaiman Bayani | a. Babu wani martanin da ya shafi kwayoyin halittar ɗan adam, sauran ƙwayoyin cuta na ƙwayoyin cuta na tarin fuka da kuma ƙwayoyin cutar huhu da wannan kayan aikin ya gano.b. An gano wuraren maye gurbi na wasu kwayoyin halitta masu jure wa magani a cikin tarin fuka na Mycobacterium na daji, kamar yankin da ke tantance juriya na kwayar halittar rifampicin rpoB, kuma sakamakon gwajin ya nuna babu juriya ga INH, wanda ke nuna babu amsawar giciye. |
| Kayan Aiki Masu Amfani | Tsarin PCR na SLAN-96P na GaskiyaTsarin PCR na BioRad CFX96 na GaskiyaLightCycler480®Tsarin PCR na Ainihin Lokaci |
Gudun Aiki
Idan ana amfani da Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (wanda za a iya amfani da shi tare da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. don cirewa, ƙara 200μL na samfurin sarrafawa mara kyau da aka sarrafa don a gwada su a jere, kuma a ƙara 10μL na tsarin sarrafawa na ciki daban-daban a cikin samfurin sarrafawa mara kyau, wanda aka sarrafa don a gwada, kuma matakan da ke gaba ya kamata a aiwatar su sosai bisa ga umarnin cirewa. Girman samfurin da aka cire shine 200μL, kuma girman fitarwa da aka ba da shawarar shine 100μL.
