Mycobacterium Tuberculosis Nucleic Acid da Rifampicin (RIF), Resistance (INH)

Takaitaccen Bayani:

Ana amfani da wannan kayan aiki don gano DNA na tarin fuka na Mycobacterium a cikin maniyyi na ɗan adam, al'adar solid (LJ Medium) da al'adar ruwa (MGIT Medium), ruwan wankewar bronchial, da maye gurbi a cikin yankin codon amino acid 507-533 (81bp, yankin da ke tantance juriyar rifampicin) na kwayar halittar rpoB na juriyar tarin fuka ta Mycobacterium rifampicin, da kuma maye gurbi a cikin manyan wuraren maye gurbi na juriyar isoniazid na tarin fuka ta Mycobacterium. Yana taimakawa wajen gano kamuwa da cutar tarin fuka ta Mycobacterium, kuma yana gano manyan kwayoyin halittar juriya na rifampicin da isoniazid, wanda ke taimakawa wajen fahimtar juriyar magani na tarin fuka ta Mycobacterium da mara lafiya ya kamu da ita.

Aika mana imel

Cikakken Bayani game da Samfurin

Alamun Samfura

Sunan samfurin

Kayan Ganowa na HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid da Rifampicin (RIF), (INH) (Narkewar Curve)

Ilimin Cututtuka

Tarin fuka na Mycobacterium, wanda aka sani da Tubercle Bacillus (TB), shine kwayar cuta mai saurin yaɗuwa da ke haifar da tarin fuka, kuma a halin yanzu, magungunan farko da ake amfani da su wajen yaƙi da tarin fuka sun haɗa da isoniazid, rifampicin da ethambutol, da sauransu.^[1]Duk da haka, saboda rashin amfani da magungunan hana tarin fuka ba daidai ba da kuma halayen tsarin bangon tantanin halitta na tarin fuka na mycobacterium da kanta, tarin fuka na mycobacterium ya haɓaka juriya ga magunguna ga magungunan hana tarin fuka, kuma wani nau'i mai haɗari musamman shine tarin fuka mai jure magunguna da yawa (MDR-TB), wanda ke jure wa magunguna biyu mafi yawan gaske kuma masu tasiri, rifampicin da isoniazid.^[2].

Matsalar juriyar magungunan tarin fuka tana nan a duk ƙasashen da WHO ta yi bincike a kansu. Domin samar da ingantattun tsare-tsare na magani ga masu fama da tarin fuka, ya zama dole a gano juriyar magungunan rigakafin tarin fuka, musamman juriyar rifampicin, wanda ya zama matakin gano cutar da WHO ta ba da shawarar yi wajen magance tarin fuka.^[3]Duk da cewa gano juriyar rifampicin kusan yayi daidai da gano MDR-TB, gano juriyar rifampicin kawai yana yin watsi da marasa lafiya da ke da juriyar mono-resistant INH (wanda ke nufin juriyar isoniazid amma mai saurin kamuwa da rifampicin) da kuma rifampicin mai saurin kamuwa da mono-resistant (mai saurin kamuwa da isoniazid amma mai saurin kamuwa da rifampicin), wanda zai iya haifar da marasa lafiya da aka yi musu magani mara kyau na farko. Saboda haka, gwajin juriyar isoniazid da rifampicin su ne mafi ƙarancin buƙatun da ake buƙata a duk shirye-shiryen kula da DR-TB.^[⁴^].

Sigogi na Fasaha

Ajiya	≤-18℃
Tsawon lokacin shiryawa	Watanni 12
Nau'in Samfuri	Samfurin Maniyyi, Al'ada Mai Kyau (Matsakaicin LJ), Al'adar Ruwa (Matsakaicin MGIT)
CV	<5.0%
LoD	LoD na kayan aikin gano tarin fuka na Mycobacterium shine ƙwayoyin cuta 10/mL;LoD na kayan aikin gano nau'in daji na rifampicin da nau'in maye gurbi shine ƙwayoyin cuta 150/mL; LoD na kayan aikin gano nau'in daji na isoniazid da nau'in maye gurbi shine ƙwayoyin cuta 200/mL.
Takamaiman Bayani	1) Babu wani martanin giciye yayin amfani da kayan aikin don gano DNA na kwayoyin halittar ɗan adam (500ng), wasu nau'ikan cututtukan numfashi guda 28, da nau'ikan mycobacteria marasa tarin fuka guda 29 (kamar yadda aka nuna a cikin Jadawali na 3).2) Babu wani martanin giciye yayin amfani da kayan aikin don gano wuraren maye gurbi na wasu kwayoyin halittar Mycobacterium masu juriya ga magunguna na rifampicin da isoniazid (kamar yadda aka nuna a Jadawali na 4).3) Magungunan da ake amfani da su wajen hana shiga cikin samfuran da za a gwada, kamar su rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), zanamivir (0.5mg/L), dexamethasone (20mg/L), ba su da wani tasiri a kan sakamakon gwajin kayan.
Kayan Aiki Masu Amfani	Tsarin PCR na SLAN-96P na Gaskiya (Hongshi Medical Technology Co., Ltd.), Tsarin PCR na BioRad CFX96 na Ainihin Lokaci