Mycobacterium Tuberculosis Nucleic Acid da Rifampicin (RIF) , Resistance (INH)

Takaitaccen Bayani:

Ana amfani da wannan kit ɗin don in vitro qualitative detection na Mycobacterium tarin fuka DNA a cikin ɗan adam sputum, m al'ada (LJ Medium) da ruwa al'ada (MGIT Medium), Bronchial lavage ruwa ruwa, da kuma maye gurbi a cikin 507-533 amino acid codon yankin (81bp, rifampicin juriya kayyade yanki na Mycobacterium, da juriya rifampicin yankin) na rcopoB. maye gurbi a cikin manyan wuraren maye gurbi na Mycobacterium tarin fuka isoniazid resistance.Yana bayar da taimako ga ganewar cutar ta Mycobacterium tarin fuka, kuma yana gano babban juriya na kwayoyin rifampicin da isoniazid, wanda ke taimakawa wajen fahimtar juriyar magungunan Mycobacterium tarin fuka da marasa lafiya suka kamu da su.

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HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid da Rifampicin(RIF), (INH) Kayan Ganewa (Narkewar Curve)

Epidemiology

Mycobacterium tarin fuka, jim kadan a matsayin Tubercle bacillus (TB), ita ce kwayar cutar da ke haifar da tarin fuka, kuma a halin yanzu, magungunan da ake amfani da su na farko na maganin tarin fuka sun hada da isoniazid, rifampicin da ethambutol, da dai sauransu.^[1]. Duk da haka, saboda rashin amfani da maganin tarin fuka da kuma halayen tsarin bangon cell na tarin fuka da kansa, mycobacterium tarin fuka ya haɓaka maganin maganin maganin tarin fuka, kuma wani nau'i mai haɗari na musamman shine ƙwayar tarin fuka mai yawa (MDR-TB), wanda yake jure wa magungunan da aka fi sani da kuma tasiri, rifani da kuma rifazid.^[2].

Matsalar juriyar maganin tarin fuka tana wanzuwa a duk ƙasashen da WHO ta yi bincike. Domin samar da ingantattun tsare-tsare na kula da masu fama da cutar tarin fuka, ya zama dole a gano juriyar maganin tarin fuka, musamman juriya na rifampicin, wanda ya zama matakin tantancewa da hukumar lafiya ta WHO ta ba da shawarar wajen kula da cutar tarin fuka.^[3]. Duk da cewa gano juriyar rifampicin kusan ya yi daidai da gano MDR-TB, gano juriyar rifampicin kawai ya yi watsi da marasa lafiya da ke da INH mai juriya ta mono-resistant (wanda ke nufin juriya ga isoniazid amma mai kula da rifampicin) da kuma rifampicin mai juriya na mono-resistant (hankalin isoniazid amma juriya ga rifampicin), wanda zai iya haifar da marasa lafiya da ba za a iya magance su ba. Don haka, gwajin juriya na isoniazid da rifampicin sune mafi ƙarancin buƙatun buƙatu a cikin duk shirye-shiryen sarrafa DR-TB^[⁴^].

Ma'aunin Fasaha

Adana	≤-18℃
Rayuwar rayuwa	watanni 12
Nau'in Samfura	Samfurin Sputum, Tsayayyen Al'adu (Matsakaicin LJ), Al'adun Liquid (Matsakaicin MGIT)
CV	<5.0%
LoD	LoD na kit don gano tarin fuka na Mycobacterium shine kwayoyin cuta 10 / ml;LoD na kit don gano nau'in daji na rifampicin da nau'in mutant shine kwayoyin 150 / ml; LoD na kit don gano nau'in daji na isoniazid da nau'in mutant shine kwayoyin cuta 200 / ml.
Musamman	1) Babu wani giciye yayin amfani da kit ɗin don gano DNA ɗin ɗan adam (500ng), sauran nau'ikan cututtukan numfashi guda 28, da nau'ikan mycobacteria marasa tarin fuka guda 29 (kamar yadda aka nuna a cikin Table 3).2) Babu wani ra'ayi na giciye yayin amfani da kit don gano wuraren maye gurbi na sauran kwayoyin halittar rifampicin masu jure wa magani da tarin fuka na isoniazid (kamar yadda aka nuna a Table 4).3) Abubuwan da ke shiga tsakani na yau da kullun a cikin samfuran da za a gwada, kamar su rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide), (45mg. (20mg/L) kwayoyi, ba su da tasiri akan sakamakon gwajin kit.
Abubuwan da ake Aiwatar da su	SLAN-96P Tsarin PCR na Gaskiya (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Tsarin PCR na Gaskiya