Cutar Zika
Sunan samfur
HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Takaddun shaida
CE
Epidemiology
Kwayar cutar Zika na cikin dangin Flaviviridae ne, kwayar cutar RNA ce mai tsini guda daya da diamita na 40-70nm. Yana da ambulaf, ya ƙunshi 10794 nucleotides, kuma yana ɓoye 3419 amino acid. Bisa ga genotype, an raba shi zuwa nau'in Afirka da na Asiya. Cutar kwayar cutar Zika cuta ce mai saurin kamuwa da cuta wacce cutar Zika ke haifar da ita, wacce galibi ke yaduwa ta hanyar cizon sauro Aedes aegypti. Siffofin asibiti sun hada da zazzabi, kurji, arthralgia ko conjunctivitis, kuma ba kasafai ake yin kisa ba. A cewar Hukumar Lafiya ta Duniya, microcephaly na jarirai da ciwon Guillain-Barre (Guillain-Barré syndrome) na iya haɗuwa da kamuwa da cutar Zika.
Tashoshi
| FAM | Zika virus nucleic acid |
| ROX | Ikon Cikin Gida |
Ma'aunin Fasaha
| Adana | ≤30℃ & kariya daga haske |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | sabobin magani |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 Kwafi/ml |
| Musamman | Yi amfani da kit ɗin don gano samfuran jini tare da ƙwayar cutar Zika mara kyau, kuma sakamakon ba shi da kyau. Sakamakon gwajin tsangwama ya nuna cewa lokacin da adadin bilirubin a cikin jini bai wuce 168.2μmol/ml ba, yawan haemoglobin da ake samu ta hanyar hemolysis bai wuce 130g/L ba, adadin lipid na jini bai wuce 65mmol/ml ba, jimillar IgG a cikin jini bai wuce 5mg/mL ba, babu wani tasiri a kan kwayar cutar dengue ko kwayar cutar Zikun. Kwayar cutar Hepatitis A, Hepatitis B Virus, Hepatitis C Virus, Herpes Virus, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile virus da kuma jinin jikin dan adam an zabo don gwajin amsawa, kuma sakamakon ya nuna cewa babu wani abu tsakanin wannan kit da cututtukan da aka ambata a sama. |
| Abubuwan da ake Aiwatar da su | ABI 7500 Tsarin PCR na GaskiyaTsarin PCR na ABI 7500 Mai Saurin Gaskiya QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Real-Time PCR tsarin LineGene 9600 Plus Tsarin Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Zabin 1.
QIAamp Viral RNA Mini Kit(52904), Nucleic Acid Extraction ko Tsarkake Reagent (YDP315-R) ta Tiangen Biotech(Beijing) Co., Ltd.The hakarya kamata a fitar da shi bisa ga umarnin cirewa, kuma ƙimar da aka ba da shawarar ita ce 140 μL kuma ƙimar da aka ba da shawarar ita ce 60 μL.
Zabin 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006). Ya kamata a fitar da hakar bisa ga umarnin. Girman samfurin cirewa shine 200 μL, kuma ƙimar da aka ba da shawarar shine 80μL.
