Cutar Zika
Sunan samfurin
Kayan Gano Acid na Nucleic na HWTS-FE002 na Cutar Zika (Fluorescence PCR)
Takardar Shaidar
CE
Ilimin Cututtuka
Kwayar cutar Zika ta kasance daga cikin dangin Flaviviridae, kwayar cutar RNA ce mai siffar RNA mai siffar positive guda ɗaya mai diamita na 40-70nm. Tana da ambulaf, tana ɗauke da nucleotides 10794, kuma tana ɗauke da amino acid 3419. Dangane da nau'in halittar, an raba ta zuwa nau'in Afirka da na Asiya. Cutar kwayar cutar Zika cuta ce mai saurin yaɗuwa wacce kwayar cutar Zika ke haifarwa, wacce galibi ake yaɗa ta ta hanyar cizon sauro na Aedes aegypti. Sifofin cutar galibi su ne zazzabi, kurji, arthralgia ko conjunctivitis, kuma ba kasafai take kashe mutum ba. A cewar Hukumar Lafiya ta Duniya, ƙananan ƙwayoyin cuta na jarirai da kuma ciwon Guillain-Barre (Guillain-Barré syndrome) na iya kasancewa da alaƙa da kamuwa da cutar Zika.
Tashar
| FAM | Kwayar cutar Zika mai suna nucleic acid |
| ROX | Sarrafa Cikin Gida |
Sigogi na Fasaha
| Ajiya | ≤30℃ & kariya daga haske |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | sabon magani |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | Kwafi 500/mL |
| Takamaiman Bayani | Yi amfani da kayan aikin don gano samfuran jini da ke ɗauke da cutar Zika mara kyau, kuma sakamakon ya kasance mara kyau. Sakamakon gwajin tsangwama ya nuna cewa lokacin da yawan bilirubin a cikin jini bai wuce 168.2μmol/ml ba, yawan haemoglobin da hemolysis ke samarwa bai wuce 130g/L ba, yawan lipids a cikin jini bai wuce 65mmol/ml ba, jimlar yawan IgG a cikin jini bai wuce 5mg/mL ba, babu wani tasiri ga kwayar cutar dengue, kwayar cutar Zika ko gano kwayar cutar chikungunya. An zaɓi ƙwayar cutar Hepatitis A, ƙwayar cutar Hepatitis B, ƙwayar cutar Hepatitis C, ƙwayar cutar Herpes, ƙwayar cutar Eastern equine encephalitis, ƙwayar cutar Hantavirus, ƙwayar cutar Bunya, ƙwayar cutar West Nile da samfuran kwayar halittar ɗan adam don gwajin amsawar giciye, kuma sakamakon ya nuna cewa babu wani martani tsakanin wannan kayan aikin da ƙwayoyin cuta da aka ambata a sama. |
| Kayan Aiki Masu Amfani | Tsarin PCR na ABI 7500 na GaskiyaTsarin PCR na ABI 7500 Mai Sauri a Lokaci-lokaci QuantStudio®Tsarin PCR guda 5 na Ainihin Lokaci Tsarin PCR na SLAN-96P na Gaskiya LightCycler®Tsarin PCR na 480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokaci Mai Keke Mai Zafi na MA-6000 na Ainihin Lokaci Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Zaɓi na 1.
Kit ɗin QIAamp Viral RNA Mini (52904), Cirewar Nucleic Acid ko Reagent na Tsarkakewa (YDP315-R) ta Tiangen Biotech(Beijing) Co.,Ltd.Fitarwarya kamata a cire shi bisa ga umarnin cirewa, kuma girman cirewa da aka ba da shawarar shine 140 μL kuma girman cirewa da aka ba da shawarar shine 60 μL.
Zaɓi na 2.
Kit ɗin DNA/RNA na Macro & Micro-Test Viral (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Ya kamata a cire cirewar bisa ga umarnin. Girman samfurin cirewar shine 200 μL, kuma girman fitarwa da aka ba da shawarar shine 80 μL.
