Mutum BRAF Gene V600E Mutation
Sunan samfur
HWTS-TM007-Human BRAF Gene V600E Kayan Gane Maɓalli (Fluorescence PCR)
Takaddun shaida
CE/TFDA
Epidemiology
An samo fiye da nau'in 30 na maye gurbi na BRAF, wanda kusan kashi 90 cikin dari suna cikin exon 15, inda ake ganin maye gurbin V600E shine maye gurbi na yau da kullum, wato, thymine (T) a matsayi na 1799 a exon 15 an canza shi zuwa adenine (A), wanda ya haifar da maye gurbin valine (V) a matsayi na 6. Ana samun maye gurbin BRAF a cikin muggan ciwace-ciwace irin su melanoma, ciwon daji na launi, ciwon daji na thyroid, da kansar huhu. Fahimtar maye gurbin kwayar halittar BRAF ya zama buƙatar tantance EGFR-TKIs da magungunan maye gurbi na BRAF a cikin magungunan da aka yi niyya na asibiti ga marasa lafiya waɗanda za su iya amfana.
Tashoshi
| FAM | maye gurbin V600E, kulawar ciki |
Ma'aunin Fasaha
| Adana | ≤-18℃ |
| Rayuwar rayuwa | watanni 9 |
| Nau'in Samfura | paraffin-inbedded pathological nama samfurori |
| CV | 5.0% |
| Ct | ≤38 |
| LoD | Yi amfani da kayan aikin don gano madaidaicin ingancin ingancin LoD. a) ƙarƙashin nau'in nau'in daji na 3ng / μL, ana iya gano ƙimar maye gurbin 1% a cikin buffer mai ƙarfi; b) ƙarƙashin 1% maye gurbi, maye gurbi na 1 × 103Kwafi/ml a cikin nau'in daji na 1 × 105Ana iya gano kwafi/ml a tabbatattu a cikin buffer dauki; c) IC Reaction Buffer na iya gano mafi ƙasƙanci gano iyaka ingancin iko SW3 na kamfani na cikin gida. |
| Kayayyakin aiki: | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na GaskiyaAiwatar da Biosystems 7300 Real-Time PCR Tsarukan, QuantStudio® 5 Tsarin PCR na Gaskiya LightCycler®480 Tsarin PCR na Gaskiya BioRad CFX96 Tsarin PCR na Gaskiya |
Gudun Aiki
Abubuwan da aka ba da shawarar cirewa: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Kayan aikin Tissue DNA Rapid Extraction Kit (DP330) wanda Tiangen Biotech(Beijing) Co., Ltd ke ƙera.
