Canjin Halittar Dan Adam ta BRAF V600E
Sunan samfurin
Kayan Gano Canjin Halittar HWTS-TM007-Human BRAF Gene V600E (Fluorescence PCR)
Takardar Shaidar
CE/TFDA
Ilimin Cututtuka
An gano nau'ikan maye gurbi sama da 30 na BRAF, waɗanda kusan kashi 90% suna cikin exon 15, inda ake ɗaukar maye gurbi na V600E a matsayin maye gurbi mafi yawan lokuta, wato, thymine(T) a matsayi na 1799 a cikin exon 15 an canza shi zuwa adenine (A), wanda ke haifar da maye gurbi na valine (V) a matsayi na 600 da glutamic acid (E) a cikin samfurin furotin. Sauye-sauyen BRAF galibi ana samun su a cikin ciwace-ciwacen daji kamar melanoma, ciwon daji na hanji, ciwon thyroid, da ciwon huhu. Fahimtar maye gurbi na kwayar halittar BRAF ya zama buƙatar tantance magungunan maye gurbi na EGFR-TKIs da magungunan maye gurbi na BRAF da aka yi niyya a cikin maganin magunguna na asibiti ga marasa lafiya waɗanda za a iya amfana da su.
Tashar
| FAM | Sauye-sauyen V600E, sarrafa ciki |
Sigogi na Fasaha
| Ajiya | ≤-18℃ |
| Tsawon lokacin shiryawa | Watanni 9 |
| Nau'in Samfuri | samfuran nama na paraffin da aka saka |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | Yi amfani da kayan aikin don gano daidaitaccen tsarin kula da ingancin LoD. a) a ƙarƙashin asalin nau'in daji na 3ng/μL, ana iya gano ƙimar maye gurbin 1% a cikin ma'aunin amsawa cikin kwanciyar hankali; b) ƙasa da ƙimar maye gurbin 1%, maye gurbin 1×103Kwafi/mL a cikin asalin daji na 1×105Ana iya gano kwafi/mL a hankali a cikin ma'ajiyar amsawa; c) Mai riƙe da IC Reaction Buffer zai iya gano ƙarancin ikon sarrafa ingancin ganowa SW3 na ikon sarrafa cikin gida na kamfanin. |
| Kayan Aiki Masu Amfani: | Tsarin PCR na Zamani na Aiyuka 7500Tsarin Halittar Halittu Masu Amfani 7300 PCR na Lokaci-lokaci Tsarin, Tsarin PCR na QuantStudio® 5 na Ainihin Lokaci Tsarin PCR na LightCycler®480 na Ainihin Lokaci Tsarin PCR na BioRad CFX96 na Ainihin Lokaci |
Gudun Aiki
Shawarwarin sinadaran cirewa: Kit ɗin Tissue na QIAamp DNA FFPE na QIAGEN (56404), Kit ɗin cirewa na DNA mai saurin cirewa na paraffin (DP330) wanda Tiangen Biotech(Beijing) Co., Ltd. suka ƙera.
