Kayan Gano Acid na Staphylococcus Aureus da Methicillin Mai Juriya ga Staphylococcus Aureus Nucleic
Sunan samfurin
Kayan Gano Acid na Nucleic da Staphylococcus Aureus Mai Juriya ga Methicillin (PCR mai haske)
Amfani da aka yi niyya
Ana amfani da wannan kayan aiki don gano staphylococcus aureus da kuma ƙwayoyin halitta masu juriya ga methicillin a cikin samfuran maniyyi na ɗan adam, samfuran maniyyi na hanci da samfuran kamuwa da fata da nama mai laushi a cikin vitro. Ana amfani da gwajin samfurin maniyyi da maniyyi a cibiyoyin kiwon lafiya don hana da kuma sarrafa kamuwa da cutar MRSA a cikin marasa lafiya da ke asibiti, gami da marasa lafiya masu kulawa mai zurfi, marasa lafiya da aka yi wa tiyata da marasa lafiya na dogon lokaci; Hakanan ana iya amfani da shi don gano wasu marasa lafiya da ake zargi da kamuwa da cutar Staphylococcus Aureus da Methicillin-Resistant Staphylococcus Aureus; Ko kuma tare da wasu gwaje-gwajen dakin gwaje-gwaje kamar al'adun ƙwayoyin cuta don taimakawa wajen gano cututtukan fata da nama mai laushi na MRSA/SA. Ba a amfani da sakamakon gwaji kai tsaye don jagorantar amfani da maganin rigakafi.
Ilimin Cututtuka
Staphylococcus aureus yana ɗaya daga cikin mahimman ƙwayoyin cuta masu haifar da kamuwa da cuta ta hanyar jima'i. Staphylococcus aureus (SA) na cikin staphylococcus kuma yana wakiltar ƙwayoyin cuta masu kyau na Gram-positive, waɗanda zasu iya samar da nau'ikan guba da enzymes masu shiga jiki. Kwayoyin cuta suna da halaye na yaɗuwa mai faɗi, ƙarfin ƙwayoyin cuta da kuma ƙarfin juriya mai yawa. Tsarin kwayar halitta mai zafi (nuc) kwayar halitta ce ta staphylococcus aureus da aka kiyaye sosai.
A cikin 'yan shekarun nan, saboda yawan amfani da hormones da shirye-shiryen rigakafi da kuma amfani da magungunan rigakafi masu faɗi-faɗi, cututtukan da ke haifar da Staphylococcus aureus (MRSA) mai juriya ga Methicillin a cikin Staphylococcus suna ƙaruwa. Matsakaicin ƙimar gano MRSA na ƙasa shine kashi 30.2% a cikin 2019 a China. An raba MRSA zuwa MRSA mai alaƙa da kiwon lafiya (HA-MRSA), MRSA mai alaƙa da al'umma (CA-MRSA), da MRSA mai alaƙa da dabbobi (LA-MRSA). CA-MRSA, HA-MRSA, LA-MRSA suna da manyan bambance-bambance a cikin ilimin ƙwayoyin cuta, juriya ga ƙwayoyin cuta (misali, HA-MRSA yana nuna juriya ga magunguna da yawa fiye da CA-MRSA) da halayen asibiti (misali wurin kamuwa da cuta). Dangane da waɗannan halaye, ana iya bambanta CA-MRSA da HA-MRSA. Duk da haka, bambance-bambancen da ke tsakanin CA-MRSA da HA-MRSA suna raguwa saboda yawan motsi tsakanin mutane tsakanin asibitoci da al'ummomi. MRSA yana jure wa magunguna da yawa, ba wai kawai yana jure wa maganin rigakafi na β-lactam ba, har ma da aminoglycosides, macrolides, tetracyclines da quinolones a matakai daban-daban. Akwai manyan bambance-bambance a yankuna a cikin yawan juriya ga magunguna da kuma yanayin da ake ciki daban-daban. MRSA na iya mamaye jiki ta hanyar raunukan fata, gashin gashi, jini da sauran abubuwan da ke haifar da kamuwa da cuta, kuma marasa lafiya da cututtukan fata da ƙonewa suna da matuƙar saurin kamuwa da kamuwa da cutar MRSA. Ciwon huhu yana ɗaya daga cikin cututtukan asibiti da aka fi kamuwa da su a MRSA, kuma magani mara kyau muhimmin abu ne na yawan mace-mace.
Sigogi na Fasaha
| Ajiya | ≤ -18℃ |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | Man shafawa, Hanci, Samfurin kamuwa da cutar fata da tausasa nama, |
| CV | <5.0% |
| LoD | 1000 CFU/mL |
| Takamaiman Bayani | Gwajin amsawar giciye ya nuna cewa wannan kayan aikin ba shi da amsawar giciye tare da wasu cututtukan numfashi kamar methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Kayan Aiki Masu Amfani | Nau'i na I: Tsarin Biosystems 7500 na Gaskiya-Tsakiya na PCR, Tsarin QuantStudio®5 na Gaskiya-Tsakiya na PCR, Tsarin SLAN-96P na Gaskiya-Tsakiya na PCR (Hongshi Medical Technology Co., Ltd.), Tsarin Gano PCR na Gaskiya-Tsakiya na LineGene 9600 Plus (FQD-96A, fasahar Hangzhou Bioer), MA-6000 Mai Zane-zanen Zafin Zamani na Gaskiya-Tsakiya (Suzhou Molarray Co., Ltd.), Tsarin BioRad CFX96 na Gaskiya-Tsakiya na PCR, Tsarin BioRad CFX Opus 96 na Gaskiya-Tsakiya na PCR. Nau'i na II: HWTS-EudemonTM AIO800 |
Gudun Aiki
Ana buƙatar reagent da kayan aiki amma ba a samar da su ba
Nau'i na I
(1) Kayan Aikin DNA/RNA na Babban Gwaji na Macro & Micro-Gwaji (HWTS-3019) daga Jiangsu Macro & Micro-Gwaji Med-Tech Co., Ltd. (Ana iya amfani da shi tare da Macro & Micro-Gwaji Mai Cire Acid na Atomatik (HWTS-3006B, 3006C).
(2) Samfurin mai narkewa: 4% NaOH ko Sputasol daga Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (STXB 20220253)
(3) Ruwan gishiri na yau da kullun.
Nau'i na II:
Kit ɗin DNA/RNA na Jiangsu Macro & Micro-Test na Babban Gwaji da Ƙananan Gwaji (HWTS-3019-8) daga Kamfanin Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Ana buƙatar kayan amfani amma ba a bayar da su ba:Nasihohi marasa RNase/DNase, safar hannu da za a iya zubarwa, bututun EP marasa RNase/DNase, tube 8 na PCR.
