Staphylococcus Aureus da Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
Sunan samfur
HWTS-OT062 Staphylococcus Aureus da Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Kayan Gano Acid Nucleic (Fluorescence PCR)
Takaddun shaida
CE
Epidemiology
Staphylococcus aureus yana daya daga cikin mahimman kwayoyin cuta na kamuwa da cuta na nosocomial.Staphylococcus aureus (SA) na cikin staphylococcus ne kuma wakili ne na kwayoyin cutar Gram-positive, wanda zai iya samar da nau'i na gubobi da enzymes masu lalata.Kwayoyin cuta suna da halaye na rarrabawa mai yawa, ƙaƙƙarfan pathogenicity da babban juriya.Halin da ake iya ɗauka na nuclease (nuc) wani ƙwayar cuta ce ta staphylococcus aureus.
Tashoshi
| FAM | kwayoyin mecA mai jurewa methicillin |
| ROX | Ikon Cikin Gida |
| CY5 | staphylococcus aureus nuc gene |
Ma'aunin Fasaha
| Adana | ≤-18℃ & kariya daga haske |
| Rayuwar rayuwa | watanni 12 |
| Nau'in Samfura | sputum, fata da taushi nama samfurin kamuwa da cuta, da hanci swab samfurori |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/ml staphylococcus aureus, 1000 CFU/mL kwayoyin cutar methicillin.Lokacin da kit ɗin ya gano bayanin LoD na ƙasa, 1000/ml staphylococcus aureus za a iya gano shi. |
| Musamman | Gwajin giciye-reactivity ya nuna cewa wannan kit ɗin ba shi da giciye tare da sauran ƙwayoyin cuta na numfashi kamar methicillin-sensitive staphylococcus aureus, coagulase-korau staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, pseudomonas aeruginosa, escherichia coli, pseudomonas aeruginosa. Mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Abubuwan da ake Aiwatar da su | Abubuwan da aka Aiwatar da Biosystems 7500 Tsarin PCR na Gaskiya QuantStudio®5 Tsarin PCR na Gaskiya SLAN-96P Tsarin PCR na Gaskiya LightCycler®480 Real-Time PCR tsarin LineGene 9600 Plus Tsarin Gane PCR na Gaskiya MA-6000 Mai ƙididdige yawan zafin jiki na ainihi BioRad CFX96 Tsarin PCR na Gaskiya BioRad CFX Opus 96 Tsarin PCR na Gaskiya |
Gudun Aiki
Zabin 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ana iya amfani dashi tare da Macro & Micro-Test Atomatik Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Ƙara 200µL na saline na al'ada zuwa hazo da aka sarrafa, kuma matakan da suka biyo baya ya kamata a fitar da su bisa ga umarnin, kuma ƙarar da aka ba da shawarar shine 80µL.
Zabin 2.
Macro & Micro-Test Samfurin Sakin Reagent (HWTS-3005-8) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ƙara 1mL na Saline na al'ada zuwa hazo bayan wankewa da salin na al'ada, sannan a gauraya da kyau.Centrifuge a 13,000r/min na tsawon mintuna 5, cire maɗaukaki (ajiye 10-20µL na supernatant), kuma bi umarnin don hakar na gaba.
Shawarar hakar reagent: Nucleic Acid Extraction ko Purification Reagent (YDP302) ta Tiangen Biotech (Beijing) Co., Ltd.Ya kamata a aiwatar da hakar sosai bisa ga mataki na 2 na littafin koyarwa.Ana ba da shawarar yin amfani da RNase da ruwa maras DNase don haɓakawa tare da ƙarar 100µL.
