Staphylococcus Aureus da Staphylococcus Aureus Mai Juriya Ga Methicillin (MRSA/SA)
Sunan samfurin
Kayan Gano Acid na Nucleic (PCR mai haske) na HWTS-OT062 Staphylococcus Aureus da Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
Takardar Shaidar
CE
Ilimin Cututtuka
Staphylococcus aureus yana ɗaya daga cikin mahimman ƙwayoyin cuta masu haifar da kamuwa da cuta ta hanyar jima'i. Staphylococcus aureus (SA) na cikin staphylococcus kuma yana wakiltar ƙwayoyin cuta masu kyau na Gram-positive, waɗanda zasu iya samar da nau'ikan guba da enzymes masu shiga jiki. Kwayoyin cuta suna da halaye na yaɗuwa mai faɗi, ƙarfin ƙwayoyin cuta da kuma ƙarfin juriya mai yawa. Tsarin kwayar halitta mai zafi (nuc) kwayar halitta ce ta staphylococcus aureus da aka kiyaye sosai.
Tashar
| FAM | kwayar halittar mecA mai jure wa methicillin |
| ROX | Sarrafa Cikin Gida |
| CY5 | kwayar halittar staphylococcus aureus nuc |
Sigogi na Fasaha
| Ajiya | ≤-18℃ & kariya daga haske |
| Tsawon lokacin shiryawa | Watanni 12 |
| Nau'in Samfuri | man shafawa, samfuran kamuwa da cutar fata da nama mai laushi, da samfuran shafawar hanci |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, ƙwayoyin cuta masu jure wa methicillin 1000 CFU/mL. Idan kayan aikin ya gano ma'aunin LoD na ƙasa, ana iya gano staphylococcus aureus 1000/mL |
| Takamaiman Bayani | Gwajin amsawar giciye ya nuna cewa wannan kayan aikin ba shi da amsawar giciye tare da wasu cututtukan numfashi kamar methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Kayan Aiki Masu Amfani | Tsarin PCR na Zamani na Aiyuka 7500 QuantStudio®Tsarin PCR guda 5 na Ainihin Lokaci Tsarin PCR na SLAN-96P na Gaskiya LightCycler®Tsarin PCR na 480 na Ainihin Lokaci Tsarin Gano PCR na Layin Ganowa na Layin Ganowa na 9600 Plus na Lokaci-lokaci Mai Keke Mai Zafi na MA-6000 na Ainihin Lokaci Tsarin PCR na BioRad CFX96 na Ainihin Lokaci Tsarin PCR na BioRad CFX Opus 96 na Ainihin Lokaci |
Gudun Aiki
Zaɓi na 1.
Ana iya amfani da Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) ta Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. tare da Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ƙara 200µL na gishirin yau da kullun a cikin ruwan da aka sarrafa, kuma ya kamata a cire matakan da ke gaba bisa ga umarnin, kuma girman fitar da ruwa da aka ba da shawarar shine 80µL.
Zaɓi na 2.
Maganin Sample Release na Macro & Micro-Test (HWTS-3005-8) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ƙara 1mL na gishirin yau da kullun a cikin abin da ya fashe bayan an wanke shi da gishirin yau da kullun, sannan a gauraya sosai. A naɗe a cikin injin centrifuge a 13,000r/min na tsawon mintuna 5, a cire ruwan da ya ɓace (a ajiye 10-20µL na ruwan da ya ɓace), sannan a bi umarnin don cirewa daga baya.
Maganin cirewa da aka ba da shawarar: Cirewar Acid ta Nucleic ko Tsarkakewa (YDP302) ta Tiangen Biotech (Beijing) Co.,Ltd. Ya kamata a yi cirewar bisa ga mataki na 2 na littafin umarnin. Ana ba da shawarar a yi amfani da ruwan RNase da DNase don cirewa tare da girman 100µL.
