Stafyloccus Aureus da Meticillin-Juyin StaphylocCus Aureus (MRSA / Sa)
Sunan Samfuta
Hwts-ot062 staphylocus Aureus Staphillin-resistant Staphyloccus Aureus (MRSA / Sa)
Takardar shaida
CE
TopideMology
Stafyloccus Aureus yana ɗaya daga cikin mahimman ƙwayoyin cuta na kamuwa da cuta na ciki. Stafyloccus Aureus (SA) ne na ƙwayoyin cuta kuma shine wakilin gram-tabbatacce, wanda zai iya samar da nau'ikan gubobi da enzymes da enzymes. Kwayoyin suna da halayen rarraba wurare dabam dabam, ƙarfi da ƙarfi da kuma babban juriya. Tsarin nuclease (Nuc) ne mai kiyayewa na Staphyloccus Aureus.
Hanya
| Famfo | Meticillin-mai tsayayya wa gene |
| Rox | Kula da ciki |
| Cy5 | Staphyloccus Aureus Nuc Gane |
Sigogi na fasaha
| Ajiya | ≤-18 ℃ & kariya daga haske |
| Shelf-rayuwa | Watanni 12 |
| Nau'in samfur | sputum, fata da sutturar kamuwa da cuta na nama, da hanci swab samfurori |
| Ct | ≤36 |
| CV | ≤5.0% |
| Lod | 1000 cfu / ml stapyloccus Aureus, 1000 CFU / ml stethicillin-resistant ƙwayoyin cuta. Lokacin da kit ɗin ya gano ma'anar lod, 1000 / ml snapyloccuin Aureus za a iya gano |
| BAYANIN | Gwajin da aka gicciye na giciye ya nuna cewa wannan kabi yana ba shi da hatsar kai tare da sauran sauran patoglocy -us, coagilase-korau stuginosa, coagbayali pnagonia, coagbyobia pneugonoe, chinchiella Baumannii, mariabicis mariabilis, entiderpooccuran pneeptoccus, Barcelona, Almicans, Barcelona |
| Kayan aiki | Amfani da biosystems 7500 Tsarin aiki na lokaci-lokaci Quicstatawa®5 Tsarin PCR na ainihi Slan-96p Real-Lokaci na Real-Lokaci Laima®Tsarin PCR na Real-Lokaci Linegene 9600 da tsarin ganowa na yau da kullun PCR Ma-6000 na lokaci-lokaci na zamani Tsarin cfx96 na lokaci-lokaci na lokaci-lokaci Biorad cfx opus 96 real-lokaci-lokaci tsarin PCR |
Aiki kwarara
Zabi 1.
Macro & Cor-Gwaji Denomic DNA / RNA 3006b). Add 200μl na saline na al'ada zuwa ga an sarrafa shi da shawarar, kuma matakan masu zuwa bisa ga umarnin, ƙarar ƙa'idodin ƙa'idodin shine 80μl.
Zabin 2.
Saki na gwaji na Macro & Micro-Clinent (HWTS-3005-8) By -rovy Medicu Macro & Micro-Gwaji Med-Tech Co., ltd. Add 1ml na Sirine na al'ada, to, Mix da kyau. Centrifuge a 13,000r / min na mintina 5, cire madaurin (Reserve 10-20μl na Supernant), kuma bi umarnin don mai zuwa.
Rarraba hakar da aka ba da shawarar mai zuwa: hakar kayan acid ko kuma mai saukarwa (YDP302) ta Tiaungen Biotch (Beijing) Co., Ltd. Ya kamata a sami hakar ya kamata a aiwatar da tsananin gwargwadon mataki na 2 na jagorar koyarwa. An ba da shawarar yin amfani da rnase da ruwa mai-free-kyauta don ƙa'idodin tare da girma 100μl.
